Isolation and insecticidal activities of new cyclic peptides from mangrove endophytic fungus Aspergillus sp. GXNU-4QQY1a

Tan, Meijing; Xu, Xia; Zhang, Wenxiu; Wu, Furong; Bo, Xianglong; Qin, Feng; Ju, Shichao; Song, Zishuo; Yang, Tingmi; Li, Jun; Huang, Xishan

doi:10.1016/j.fitote.2023.105693

Cited by 3 publications

(1 citation statement)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In continuing the search for structurally novel and biologically active natural products from mangrove endophytic fungi [ 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 ], a strain of Phomopsis asparagi DHS-48 isolated from the fresh root of Rhizophora mangle was found to be particularly productive with regard to the accumulation of a series of immunosuppressive chromones and cytochalasins [ 46 , 47 , 48 ]. With the goal of tapping the metabolic potential of this titled fungal strain, we initiated the co-culture fermentation process of well-characterized DHS-48 with other fungal strains.…”

Section: Introductionmentioning

confidence: 99%

Metabolomics-Guided Discovery of New Dimeric Xanthones from Co-Cultures of Mangrove Endophytic Fungi Phomopsis asparagi DHS-48 and Phomopsis sp. DHS-11

Wu,

Chen,

et al. 2024

Marine Drugs

View full text Add to dashboard Cite

The co-culture strategy, which mimics natural ecology by constructing an artificial microbial community, is a useful tool for the activation of biosynthetic gene clusters (BGCs) to generate new metabolites, as well as to increase the yield of respective target metabolites. As part of our project aiming at the discovery of structurally novel and biologically active natural products from mangrove endophytic fungi, we selected the co-culture of a strain of Phomopsis asparagi DHS-48 with another Phomopsis genus fungus DHS-11, both endophyted in mangrove Rhizophora mangle considering the impart of the taxonomic criteria and ecological data. The competition interaction of the two strains was investigated through morphology observation and scanning electron microscopy (SEM), and it was found that the mycelia of the DHS-48 and DHS-11 compacted and tangled with each other with an interwoven pattern in the co-culture system. A new approach that integrates HPLC chromatogram, 1HNMR spectroscopy, UPLC-MS-PCA, and molecular networking enabled the targeted isolation of the induced metabolites, including three new dimeric xanthones phomoxanthones L-N (1–3), along with six known analogs (4–9). Their planar structures were elucidated by an analysis of their HRMS, MS/MS, and NMR spectroscopic data and the absolute configurations based on ECD calculations. These metabolites showed broad cytotoxic activity against the cancer cells assessed, of which compounds 7–9 displayed significant cytotoxicity towards human liver cells HepG-2 with IC50 values ranging from 4.83 μM to 12.06 μM. Compounds 1–6 exhibited weak immunosuppressive activity against the proliferation of ConA-induced (T-cell) and LPS-induced (B-cell) murine splenic lymphocytes. Therefore, combining co-cultivation with a metabolomics-guided strategy as a discovery tool will be implemented as a systematic strategy for the quick discovery of target bioactive compounds.

show abstract

Section: Introductionmentioning

confidence: 99%

Metabolomics-Guided Discovery of New Dimeric Xanthones from Co-Cultures of Mangrove Endophytic Fungi Phomopsis asparagi DHS-48 and Phomopsis sp. DHS-11

Wu,

Chen,

et al. 2024

Marine Drugs

View full text Add to dashboard Cite

show abstract

Cytotoxic metabolites from the mangrove endophytic fungus Aspergillus sp. GXNU QG1a

Bo,

Zhao,

Qin

et al. 2024

Natural Product Research

View full text Add to dashboard Cite

New Secondary Metabolites of Mangrove-Associated Strains

Yu,

Wang,

Xiong

et al. 2024

Marine Drugs

View full text Add to dashboard Cite

Positioned at the dynamic interface between terrestrial and marine realms, mangroves embody a vibrant tapestry of biodiversity, encompassing an array of plants, animals, and microorganisms. These microbial inhabitants of mangrove habitats have emerged as a pivotal resource for antimicrobials and a plethora of pharmaceutically valuable compounds, spanning enzymes, antineoplastic agents, pesticides, immunosuppressants, and immunomodulators. This review delves into the recent landscape (January 2021 to May 2024, according to the time of publication) of novel secondary metabolites isolated from mangrove-associated microorganisms, analyzing 41 microbial strains that collectively yielded 165 distinct compounds. Our objective is to assess the productivity and potential of natural products derived from microbial populations within mangrove ecosystems in recent times. Notably, fungi stand out as the preeminent contributors to the emergence of these novel natural products, underscoring their pivotal role in the bioprospecting endeavors within these unique environments.

show abstract

Isolation and insecticidal activities of new cyclic peptides from mangrove endophytic fungus Aspergillus sp. GXNU-4QQY1a

Cited by 3 publications

References 25 publications

Metabolomics-Guided Discovery of New Dimeric Xanthones from Co-Cultures of Mangrove Endophytic Fungi Phomopsis asparagi DHS-48 and Phomopsis sp. DHS-11

Metabolomics-Guided Discovery of New Dimeric Xanthones from Co-Cultures of Mangrove Endophytic Fungi Phomopsis asparagi DHS-48 and Phomopsis sp. DHS-11

Cytotoxic metabolites from the mangrove endophytic fungus Aspergillus sp. GXNU QG1a

New Secondary Metabolites of Mangrove-Associated Strains

Contact Info

Product

Resources

About