Isolation and in vitro screening of plant growth promoting rhizobacteria from Barak Cenana red rice

Sukweenadhi, Johan; Purwanto, Maria Goretti Marianti; Hardjo, Popy Hartatie; Kurniawan, Geovanny; Artadana, Ida Bagus Made

doi:10.1063/1.5125541

Cited by 7 publications

(5 citation statements)

References 13 publications

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“…Phosphate solubilization by the endophytes under investigation may be important for their establishment in the rhizosphere. Previous reports have described the establishment of Alkalihalobacillus gibsonii in the rhizosphere of maize ( Batool et al, 2019 ), Achromobacter insuavis in rice ( Sukweenadhi et al, 2019 ), Terribacillus halophilus in Vitis vinifera ( Salomon et al, 2016 ), and Bacillus siamensis in maize ( Zhang et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Colonization potential of endophytes from halophytic plants growing in the “Runn of Kutch” salt marshes and their contribution to mitigating salt stress in tomato cultivation

Sahu,

Shafi,

Singh

et al. 2023

Front. Microbiol.

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Increasing soil salinity depreciates the quantity of the crop produce. Looking at the tremendous potential of plant-associated microorganisms in salinity stress mitigation, it would be very useful in exploring and deciphering salt-tolerant microorganisms from halophytic plants and their utilization in cultivated plants. With this aim, in the present study, four halophytic plants were taken from Rann of Kutch, and bacterial endophytes were isolated from different plant organs. These endophytes were characterized by plant growth and health promotion features. The molecular identification was done based on 16 s rRNA sequence similarity. It was found that the endophytic bacteria isolated from 4 different halophytes found sharing phylogenetic relatedness. Four potential endophytes Alkalihalobacillus gibsonii 2H2, Achromobacter insuavis 2H18, Terribacillus halophilus 2H20, and Bacillus siamensis 4H1 were tested in tomato for salinity stress alleviation. Changes in the levels of antioxidants were analyzed. Total chlorophyll, total phenolics, malondialdehyde, and proline content indicated reduced damage in the plant system due to salinity by the application of endophytes. All the treatments exhibited low levels of electrolyte leakage. The accumulation of enzymatic reactive oxygen species scavengers was assessed from the levels of peroxidase, catalase, superoxide dismutase, phenylalanine ammonia-lyase, ascorbate peroxidase, and guiacol peroxidase. The NBT and DAB staining confirmed the findings. The reduction in the accumulation of Na+ ions in tomato leaves was visualized using Sodium Green probes under CSLM and found to be lowest in Terribacillus halophilus 2H20 and Bacillus siamensis 4H1 inoculated plants. The endophyte Terribacillus halophilus 2H20 was the most promising isolate. The colonization in tomato roots was confirmed using a cell tracker system. Results showed that the endophytes were found to have salinity stress mitigation traits. The efficiency could be further improved with the combination of other endophytes tested earlier.

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Section: Discussionmentioning

confidence: 99%

Colonization potential of endophytes from halophytic plants growing in the “Runn of Kutch” salt marshes and their contribution to mitigating salt stress in tomato cultivation

Sahu,

Shafi,

Singh

et al. 2023

Front. Microbiol.

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“…S. maltophilia and S. pavanii have been reported to possess different PGP attributes and have also been implicated to alleviate biotic and abiotic stresses (Stamford et al, 2002;Singh and Jha, 2017;Alexander et al, 2019;Singh S. et al, 2020;Roy Chowdhury, 2020). The potential of R. pusense as PGPR has also been reported by different workers (Sukweenadhi et al, 2019;Chaudhary et al, 2021;Mir et al, 2021). Since the bioinoculants performed well upon seed inoculation, it represents high degree of rhizosphere competence which is the first and foremost requirement for developing a successful microbial inoculant (Singh DP.…”

Section: Discussionmentioning

confidence: 75%

Sulfur-Oxidizing Bacteria From Coal Mine Enhance Sulfur Nutrition in Pigeonpea (Cajanus cajan L.)

Malviya

Varma²,

Singh³

et al. 2022

Front. Environ. Sci.

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The present investigation was carried out to isolate, identify, and characterize sulfur-oxidizing bacteria (SOB) from coal mines and to evaluate the efficient strains for their ability to influence plant growth and S uptake in pigeonpea. Thirteen bacterial isolates belonging to Stenotrophomonas maltophilia (2), Stenotrophomonas pavanii (2), Rhizobium pusense (5), Bacillus velezensis (2), and Paenibacillus massiliensis (2) were obtained. Among these, seven strains that could reduce the pH of thiosulfate broth were further characterized for sulfur oxidation, plant growth-promoting (PGP) attributes, and in planta studies. Among the seven strains characterized, maximum sulfate ion was recorded for S. maltophilia DRC-18-7A (311.43 mg L−1) closely followed by S. pavanii DRC-18-7B (273.44 mg L−1) and S. maltophilia DRC-18-10 (265.75 mg L−1) after 21 days of inoculation. Among the PGP attributes quantified, maximum P solubilization was recorded in case of S. maltophilia DRC-18-7A (24.39 μg ml−1), while highest siderophore production and IAA production were recorded in S. maltophilia DRC-18-10 (14.25%) and R. pusense DRC-18-25 (15.21 μg ml−1), respectively. S. maltophilia DRC-18-7A closely followed by S. pavanii DRC-18-7B outperformed others in enhancing seed germination (%) and vigour indices. Results clearly indicated that microbial inoculants colonized the plant roots and developed biofilm on the root surface. It was further observed that plants treated with microbial inoculants induce an early formation of secondary and tertiary roots in the pigeonpea compared to the untreated control which was further confirmed by assessing the root architecture using the root scanner. Inoculation of these two strains to pigeonpea significantly enhanced plant growth parameters, the activity of reactive oxygen scavenging (ROS) enzymes, and accumulation of flavonoids, carotenoids, and proline both under sterilized and non-sterilized growth medium (sand and soil in 1:3 ratio). The application of microbial inoculants significantly increased the uptake of nitrogen, phosphorus, potassium, and sulfur in plant shoots. Further, transcript level of phosphate, potassium, and sulfur transporter genes significantly increases upon microbial inoculation leading to increased uptake and translocation of P, K, and S in the pigeonpea. The results indicate that S. maltophilia DRC-18-7A and S. pavanii DRC-18-7B could be recommended as inoculants for pigeonpea to improve its growth and sulfur nutrition.

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“…2a). In the present observation, the gradual change of color of the medium is due to gradual production of ammonia in the broth medium which increases the pH of the medium hence the dye (bromothymol blue) changed from green-yellow to blue gradually (Ishak et al, 2018;Sukweenadhi et al, 2019;Gothwal et al, 2008). Further, the bacterial isolates that could change the color of the medium were selected for quantitative ammonia production ability.…”

Section: Ammonia Production Activitymentioning

confidence: 84%

Sustainable Rice Plant Growth Promotion by Bacteria Isolated from Rhizosphere Soil

Behera,

Mahto,

Awesh Azam

et al. 2024

Jordan j. Agr. sci.

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In the present study, sixteen bacterial isolates were collected and identified from the rhizosphere soil of the bean plant (Phaseolus vulgaris), named BB-1 to BB-16. Out of the sixteen bacterial isolates, six isolates showed positive activity of phosphate solubilization ability, three bacterial isolates were found positive for ammonia production, six were positive for Indole acetic acid (IAA) production, three could solubilize potash, three bacterial isolates produced cellulase, six exhibited positive for chitinase, five were tested positive for amylase and four bacterial isolates were positive for protease activity. Hydrogen Cyanide (HCN) production was noticed by the bacterial isolates BB-7only. The fungal pathogen such as Aspergillus terreus, and Penicillium rubidurum was resistant to most bacterial isolates, whereas bacterial isolate BB-3 showed sensitivity against Penicillium rubidurum. Their quantitative phosphate solubilization ability was 47.5-77.8 µg/ml, ammonia production was between 2.45 - 3.45 mg/l, and IAA production was 22.5 -29.5 µg/ml. Of the sixteen bacterial isolates, one bacterial isolate, BB-7, was positive for most of the tests and identified as Pantoea agglomerans. Plant growth-promoting activity of the isolate showed that in comparison to the control rice plant, higher root and shoot growth was achieved in rice pot inoculated with P. agglomerans culture

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Isolation and in vitro screening of plant growth promoting rhizobacteria from Barak Cenana red rice

Cited by 7 publications

References 13 publications

Colonization potential of endophytes from halophytic plants growing in the “Runn of Kutch” salt marshes and their contribution to mitigating salt stress in tomato cultivation

Colonization potential of endophytes from halophytic plants growing in the “Runn of Kutch” salt marshes and their contribution to mitigating salt stress in tomato cultivation

Sulfur-Oxidizing Bacteria From Coal Mine Enhance Sulfur Nutrition in Pigeonpea (Cajanus cajan L.)

Sustainable Rice Plant Growth Promotion by Bacteria Isolated from Rhizosphere Soil

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