2009
DOI: 10.1007/s00418-009-0646-5
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Isolation and in vitro characterisation of dental pulp stem cells from natal teeth

Abstract: Dental pulp stem cells were primarily derived from the pulp tissues of exfoliated deciduous teeth, primary incisors and permanent third molar teeth. The aim of this study was to isolate and extensively characterise SCs derived from human natal dental pulp (hNDP). For characterisation, proliferation capacity, phenotypic properties, ultrastructural and differentiation characteristics and gene expression profiles were utilised. A comparison was done between the properties of NDP-SCs and the properties of mesenchy… Show more

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Cited by 186 publications
(209 citation statements)
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“…3B). Telomere length was not significantly (p < 0.05) different among DPaSCs, DPuSCs, DFSCs, earlier reports showing the higher expression of these specific markers in various DSCs (16,20,23,28). Howand MSCs.…”
Section: Telomere Length In Dscs and Mscsmentioning
confidence: 58%
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“…3B). Telomere length was not significantly (p < 0.05) different among DPaSCs, DPuSCs, DFSCs, earlier reports showing the higher expression of these specific markers in various DSCs (16,20,23,28). Howand MSCs.…”
Section: Telomere Length In Dscs and Mscsmentioning
confidence: 58%
“…These populations possess MSC characteristics, isolation and expansion of MSCs from bone marrow, attempts have been made to explore other alternate including the expression of cell surface markers and alkaline phosphatase (AP) activity, the capacity for selfsources. In this regard, stem cells with similar features to MSCs have been identified from dental tissues as a renewal, and multilineage differentiation potential, such as osteogenic, chondrogenic, adipogenic, myogenic, and putative stem source (11,14,20,27,40). In humans, many neurogenic lineages (12,20,40,46).…”
Section: Introductionmentioning
confidence: 99%
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“…For RT-PCR, the sense and antisense primers used are adapted from [15] and listed in (Table 1). The expression of CD146 was evaluated by flow cytometry (FACS Calibur, BD Biosciences) using an α-CD146 antibody (BD Pharmingen TM , cat.…”
Section: Cell Culture: Characterization and Osteogenic Differentiatiomentioning
confidence: 99%
“…The osteogenic commitment was identified by the expression of specific markers, such as osteonectin, and runx2 (runt-related transcription factor 2) by RT-PCR. The sense and antisense primers used are adapted from [15,16] and listed in Table 1.…”
Section: Cell Culture: Characterization and Osteogenic Differentiatiomentioning
confidence: 99%