Isolation and immunologic properties of a heterogenous antigen with the characteristics of the heavy chain of human plasma kininogen

Kärkkäinen, Tytti; Syvänen, Ann‐Christine; Turpeinen, Ursula; Hamberg, Ulla

doi:10.1016/0161-5890(82)90260-7

Molecular Immunology

1982

DOI: 10.1016/0161-5890(82)90260-7

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Isolation and immunologic properties of a heterogenous antigen with the characteristics of the heavy chain of human plasma kininogen

Tytti Kärkkäinen

Ann‐Christine Syvänen

Ursula Turpeinen

et al.

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1983

1985

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Cited by 6 publications

References 25 publications

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The role of carbohydrates in the radioimmunoassay of human low‐molecular‐mass kininogen

Turpeinen

Kärkkäinen

1985

FEBS Letters

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The immunoreactivity of human low-molecular-mass kininogen from Cohn plasma fraction IV was investigated after deglycosylations and carbohydrate modifications by radioimmunoassay using the conformationspecific antiserum. Removal of all sialic acids, 44% of amino sugars and 63% of neutral sugars did not alter the immunoreactivity of the protein but the periodate-treated concanavalin A fractions showed strikingly diminished immunoreactivity. A conformational change could account for the observed effect of periodate on the decreased reactivity of the protein in radioimmunoassay. Externally added carbohydrates had no effect on immunoreactivity. The results suggest that the carbohydrate part of kininogen is not involved in the immunoreactivity although it accounts for the observed lectin-binding heterogeneity.Carbohydrate Glycoprotein Kininogen

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The role of carbohydrates in the radioimmunoassay of human low‐molecular‐mass kininogen

Turpeinen

Kärkkäinen

1985

FEBS Letters

Self Cite

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Kininogen in factor VIII-deficient plasma (haemophilia A)

Syvänen

Hedner

Hamberg

1981

Thrombosis Research

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Conformation and sequence dependent antigenic determinants in human low molecular weight kininogen

Syvänen

Kärkkäinen

Hamberg

1983

Molecular Immunology

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Isolation and immunologic properties of a heterogenous antigen with the characteristics of the heavy chain of human plasma kininogen

Cited by 6 publications

References 25 publications

The role of carbohydrates in the radioimmunoassay of human low‐molecular‐mass kininogen

The role of carbohydrates in the radioimmunoassay of human low‐molecular‐mass kininogen

Kininogen in factor VIII-deficient plasma (haemophilia A)

Conformation and sequence dependent antigenic determinants in human low molecular weight kininogen

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