Isolation and Identification of α-Glucosidase Inhibitor From Aspergillus Terreus F38

Abstract

Controlled postprandial glucose level is an important strategy in preventing DM type 2. Inhibitors of α-glucosidase have been postulated to be useful agents in managements of DM type 2. This study aims to isolate and identify of α-glucosidase inhibitor fromAspergillus terreus F38 by liquid fermentation. The mycelium extract of A. terreus F38showed strong activity against α-glucosidase with IC 50 value of 9.65μg/mL. Separation and purification of mycelium ectract yielded compound I (Butyrolactone III). The structure was establish on the basis of spectral analysis, according to the data obtained by NMR and LCMS-MS experiments. Compound I showed potential activity against α-glucosidase with IC 50 value of 13.87 μg/mL. Therefore, the metabolites from A. terreus F38 can be used as lead compound to design potent α-glucosidase inhibitory agents.

Deciphering Molecular Aspects of Potential α-Glucosidase Inhibitors within Aspergillus terreus: A Computational Odyssey of Molecular Docking-Coupled Dynamics Simulations and Pharmacokinetic Profiling

“…Reported α -glucosidase activities regarding several A. terrus -isolated metabolites within our literature review [ 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 ] have prompted us to further investigate the potentiality of identifying novel uncited hits with potential biological activity. Additionally, exploring the molecular aspects of the compound’s affinity/binding with human α -glucosidase biological targets through a sophisticated in silico study was rationalized in order to guide future lead optimization and development.…”

Potent Natural Inhibitors of Alpha-Glucosidase and the Application of Aspergillus spp. in Diabetes type 2 Drugs: a Review

“…Chất ức chế enzyme α-glucosidase đã được phát hiện ở nhiều loài sinh vật khác nhau từ động vật, thực vật đến vi sinh vật. Tuy nhiên, nguồn phong phú cho các hợp chất AGIs vẫn là thực vật [25,26], nấm [27][28][29][30][31] và vi khuẩn [32,33].…”

Gas Chromatography-Mass Spectrometry Analysis and α-Glucosidase Inhibitory Activity of n-Hexane Extract of Bilajang Bulu (Merremia Vitifolia) Leaves

“…Inhibition of α-glucosidase assay was conducted base on the report of Munasaroh, Tamat, & Dewi (2018). As much as 4,0 mg of sample was dissolved in 500 µL of DMSO as a main solution, then diluted with DMSO in order to obtain a varied concentration of the sample at 50; 25; 12.5; 6.25 μg/mL.…”

“…The disease was characterized by a high concentration of plasma glucose, which led to major complications such as cardiovascular disease, diabetic neuropathy, and retinopathy. The number of patients increases every year all around the world, especially in low-middle income countries, including Indonesia (Kumar et al, 2011;Munasaroh et al, 2018). One of the most effective treatments for diabetes mellitus is to inhibit α-glucosidase, which inhibits glucose absorption by the epithelium membrane of the small intestine (Kumar et al, 2011).…”