Isolation and Identification of Tobacco rattle tobravirus Affecting Onion (Allium cepa L.) Plants in Egypt

Hamed, A. H.; El-Banna, O. H. M.; Ghanem, G. A. M.; Elnagaar, H.; shafie, Masoud

doi:10.3923/ijv.2012.39.49

Cited by 8 publications

(2 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The origin and genome structure of different TRV strains are quite divergent. TRV virus is ubiquitous, found in Asia [ 41 ], Africa [ 42 ], Europe [ 30 , 43 ] and America [ 12 ], demonstrating its wide range of adaptability. TRV has a bipartite RNA genome.…”

Section: Discussionmentioning

confidence: 99%

California TRV-based VIGS vectors mediate gene silencing at elevated temperatures but with greater growth stunting

2021

View full text Add to dashboard Cite

Background Tobacco rattle virus (TRV) based virus-induced gene silencing (VIGS), a widely used functional genomics tool, requires growth temperatures typically lower than those of the plant’s native environment. Enabling VIGS under native conditions in the field according to applicable safety regulations could be a revolutionary advance for ecological research. Results Here, we report the development of an enhanced thermal tolerant VIGS vector system based on a TRV California isolate. cDNA clones representing the whole viral genome were sequenced and used to construct separate binary plant transformation vectors for functional elements of RNA1 (6765 nt) and RNA2 (3682 nt). VIGS of target genes was induced by transient transformation of the host plant with both vectors or by treating the host plant with sap from already VIGS induced plants. In Nicotiana attenuata the silencing efficiency of the PDS (phytoene desaturase) gene was 90% at 28 °C and 78% at 30 °C. Silencing at these temperatures was more prominent and durable than silencing induced by the widely used TRV PpK20-based pBINTRA6/pTV00 system, but was associated with a viral phenotype. Differences in the suppressor protein and RNA dependent RNA polymerase sequences between the TRV California isolate and PpK20 may be the reason for their different thermal tolerance. Conclusions The new TRV California-based VIGS vectors induce gene silencing in Nicotiana attenuata at higher temperatures than the existing pBINTRA6/pTV00 vector system, but cause greater growth defects. The new vector system opens up an avenue to study genes functions in planta under field conditions.

show abstract

Section: Discussionmentioning

confidence: 99%

California TRV-based VIGS vectors mediate gene silencing at elevated temperatures but with greater growth stunting

2021

View full text Add to dashboard Cite

show abstract

“…The TRV RNA1, (6800 nts)) has a conserved genome organization (Nicolaisen et al 1999;Xu and Nie 2006; MacFarlane 2010). The average length of short and long particles depends on the speci c isolates, which is 48-114nm and 180-197 nm, respectively with diameter of 21-25nm (Hamed et al 2012). Corky ring-spot (CRS) disease is caused by the Tobacco rattle virus in potatoes.…”

Section: Introductionmentioning

confidence: 99%

Screening of potato cultivars to Tobacco Rattle Virus (TRV) in Azad Jammu and Kashmir-North East of Pakistan

Haneef,

Arif,

Ali

et al. 2023

Preprint

View full text Add to dashboard Cite

Azad Jammu and Kashmir (AJK) in the North-East of Pakistan is a leading seed potato producing area of the country. Potato cultivars that are grown commercially in AJK were screened against Tobacco rattle virus (TRV) under natural field conditions. The potato cultivars Bataina, Desiree, Fleminco, Kurado, Line A, Line B, Margrata, Pomola, Rodalph and Ronaldo were screened against TRV. Out of 10, four cultivars Bataina, Line B and Pomola and Ronaldo were found sensitive whereas cultivars Kurado, Margrata, Line A and Rodalph were moderately sensitive. Two cultivars Desiree and Fleminco were rated moderately insensitive, and none of the tested germplasm was completely insensitive to TRV-induced tuber necrosis. The effect of TRV on quantity (yield) and quality attributes of 10 potato cultivars were investigated in field experiments at district Poonch and Sudhnoti. Preliminary results revealed that plant height, number of stems per plant, numbers of tubers and weight of tubers were severely affected by soil-borne trichodorids transmitted TRV. It was concluded that both TRV and its vector were prevalent in major potato growing areas of the AJK and the existing potato germplasm has no resistance against the virus or its vector or both posing a serious threat to the potato production in the region. This is the first report of screening of potato germplasm against TRV in the region.

show abstract

Allium cepa (Onion)

Sastry¹,

Mandal²,

Scott³

et al. 2019

Encyclopedia of Plant Viruses and Viroids

View full text Add to dashboard Cite

Isolation and Identification of Tobacco rattle tobravirus Affecting Onion (Allium cepa L.) Plants in Egypt

Cited by 8 publications

References 6 publications

California TRV-based VIGS vectors mediate gene silencing at elevated temperatures but with greater growth stunting

California TRV-based VIGS vectors mediate gene silencing at elevated temperatures but with greater growth stunting

Screening of potato cultivars to Tobacco Rattle Virus (TRV) in Azad Jammu and Kashmir-North East of Pakistan

Allium cepa (Onion)

Contact Info

Product

Resources

About