Isolation and identification of tiger parvovirus in captive siberian tigers and phylogenetic analysis of VP2 gene

Wang, Kai; Du, Shuaishuai; Wang, Yiqi; Wang, Shaoying; Luo, Xiaoqing; Zhang, Yuanyuan; Liu, Cunfa; Wang, Haijun; Pei, Zhihua; Hu, Guang‐Wan

doi:10.1016/j.meegid.2019.103957

Cited by 12 publications

(20 citation statements)

References 30 publications

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“…Due to being replication-incompetent and producing nonpathogenic effects, VLPs have been widely used in studies of human and veterinary candidate vaccines [19]. In this study, the parvovirus VP2 gene was identified from a dead Siberian tiger in a wildlife park in the Jilin Province, China [8]. The tiger had symptoms such as diarrhea and vomiting before it died.…”

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confidence: 99%

“…Consequently, FPV has a high risk of transmission, posing a great threat to rare wild animals. In addition, FPV is prevalent in the Chinese Siberian tiger (Panthera tigris altaica) population [7,8]. On June 6th 2016, an outbreak of fatal FPV infection among captive Siberian tigers in Zhengzhou Zoo in central China was reported [7].…”

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confidence: 99%

“…On June 6th 2016, an outbreak of fatal FPV infection among captive Siberian tigers in Zhengzhou Zoo in central China was reported [7]. An FPV strain was isolated and identified from a captive Siberian Viruses 2020, 12, 315 2 of 8 tiger in a wildlife park in the Jilin Province, China [8]. Wild Siberian tigers from the Russian Far East had a 68% FPV antibody-positivity rate [9].…”

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confidence: 99%

“…The tiger had symptoms such as diarrhea and vomiting before it died. The VP2 sequence was analyzed and kindly provided by Dr. Wang [8]. We used the baculovirus expression system [20] to produce the VP2 protein of tiger FPV.…”

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confidence: 99%

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Construction and Immunogenicity of Virus-Like Particles of Feline Parvovirus from the Tiger

Jiao

Zhang

Liu

et al. 2020

Viruses

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Feline panleukopenia, caused by feline parvovirus (FPV), is a highly infectious disease characterized by leucopenia and hemorrhagic gastroenteritis that severely affects the health of large wild Felidae. In this study, tiger FPV virus-like particles (VLPs) were developed using the baculovirus expression system. The VP2 gene from an infected Siberian tiger (Panthera tigris altaica) was used as the target gene. The key amino acids of this gene were the same as those of FPV, whereas the 101st amino acid was the same as that of canine parvovirus. Indirect immunofluorescence assay (IFA) results demonstrated that the VP2 protein was successfully expressed. SDS-PAGE and Western blotting (WB) results showed that the target protein band was present at approximately 65 kDa. Electron micrograph analyses indicated that the tiger FPV VLPs were successfully assembled and were morphologically similar to natural parvovirus particles. The hemagglutination (HA) titer of the tiger FPV VLPs was as high as 1:218. The necropsy and tissue sections at the cat injection site suggested that the tiger FPV VLPs vaccine was safe. Antibody production was induced in cats after subcutaneous immunization, with a >1:210 hemagglutination inhibition (HI) titer that persisted for at least 12 months. These results demonstrate that tiger FPV VLPs might provide a vaccine to prevent FPV-associated disease in the tiger.

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confidence: 99%

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confidence: 99%

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confidence: 99%

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Construction and Immunogenicity of Virus-Like Particles of Feline Parvovirus from the Tiger

Jiao

Zhang

Liu

et al. 2020

Viruses

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“…PPV is a non-encapsulated autonomously replicating virus that belongs to the family Parvovirdae, subfamily Parvovirina, and genus Parvovirus [18]. The same genus also includes parvoviruses of cattle, cats, dogs, geese, mice, rats, tigers, rabbits, minks, chickens and raccoons [19][20][21][22][23][24]. The PPV genome is a single and negative-stranded DNA with a full length of about 5000 bp, which contains two open reading frames (ORFs) and covers the entire genome [23,25].…”

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Nanobody‑horseradish peroxidase and -EGFP fusions as reagents to detect porcine parvovirus in the immunoassays

Zhao

et al. 2020

J Nanobiotechnol

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Background: Antibodies are an important reagent to determine the specificity and accuracy of diagnostic immunoassays for various diseases. However, traditional antibodies have several shortcomings due to their limited abundance, difficulty in permanent storage, and required use of a secondary antibody. Nanobodies, which are derived from single-chain camelid antibodies, can circumvent many of these limitations and, thus, appear to be a promising substitute. In the presented study, a sandwich ELISA-like immunoassay and direct fluorescent assay with high sensitivity, good specificity, and easy operation were the first time to develop for detecting porcine parvovirus (PPV). After screening PPV viral particles 2 (VP2) specific nanobodies, horseradish peroxidase (HRP) and enhanced green fluorescent protein (EGFP) fusions were derived from the nanobodies by recombinant technology. Finally, using the nanobody-HRP and -EGFP fusions as probes, the developed immunoassays demonstrate specific, sensitive, and rapid detection of PPV.Results: In the study, five PPV-VP2 specific nanobodies screened from an immunised Bactrian camel were successfully expressed with the bacterial system and purified with a Ni-NTA column. Based on the reporter-nanobody platform, HRP and EGFP fusions were separately produced by transfection of HEK293T cells. A sandwich ELISA-like assay for detecting PPV in the samples was firstly developed using PPV-VP2-Nb19 as the capture antibody and PPV-VP2-Nb56-HRP fusions as the detection antibody. The assay showed 92.1% agreement with real-time PCR and can be universally used to surveil PPV infection in the pig flock. In addition, a direct fluorescent assay using PPV-VP2-Nb12-EGFP fusion as a probe was developed to detect PPV in ST cells. The assay showed 81.5% agreement with real-time PCR and can be used in laboratory tests. Conclusions:For the first time, five PPV-VP2 specific nanobody-HRP and -EGFP fusions were produced as reagents for developing immunoassays. A sandwich ELISA-like immunoassay using PPV-VP2-Nb19 as the capture antibody and PPV-VP2-Nb56-HRP fusion as the detection antibody was the first time to develop for detecting PPV in different samples. Results showed that the immunoassay can be universally used to surveil PPV infection in pig flock. A direct fluorescent assay using PPV-VP2-Nb12-EGFP as a probe was also developed to detect PPV in ST cells. The two developed © The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article' s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article'

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Generation and immunogenicity of virus-like particles based on mink enteritis virus capsid protein VP2 expressed in Sf9 cells

Jin

Wang

et al. 2020

Arch Virol

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Isolation and identification of tiger parvovirus in captive siberian tigers and phylogenetic analysis of VP2 gene

Cited by 12 publications

References 30 publications

Construction and Immunogenicity of Virus-Like Particles of Feline Parvovirus from the Tiger

Construction and Immunogenicity of Virus-Like Particles of Feline Parvovirus from the Tiger

Nanobody‑horseradish peroxidase and -EGFP fusions as reagents to detect porcine parvovirus in the immunoassays

Generation and immunogenicity of virus-like particles based on mink enteritis virus capsid protein VP2 expressed in Sf9 cells

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