Isolation and Identification of the Five Novel Flavonoids from Genipa americana Leaves

Silva, Larissa Marina Pereira; Alves, Jovelina Samara Ferreira; Siqueira, Emerson Michell da Silva; Neto, Manoel André de Souza; Abreu, Lucas Silva; Tavares, Josean Fechine; Porto, Dayanne Lopes; Ferreira, Leandro de Santis; Demarque, Daniel P.; Lopes, Norberto Peporine; Aragão, Cícero Flávio Soares; Zucolotto, Silvana Maria

doi:10.3390/molecules23102521

Cited by 16 publications

(9 citation statements)

References 41 publications

(86 reference statements)

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“…In the MS spectrum, peak 12 exhibited a parent ion at m/z 623[M-H] − . In the MS/MS spectrum, a base peak ion consistent with the isorhamnetin aglycone skeleton was observed at m/z 315[M-H-162-147] − due to subsequent loss of a rhamnosyl and a galactosyl residue [ 26 ]. Furthermore, there was a quercetin fragment observed at m/z 300 due to the demethylation of the isorhamnetin aglycone.…”

Section: Resultsmentioning

confidence: 99%

A Comparison of Bioactive Metabolites, Antinutrients, and Bioactivities of African Pumpkin Leaves (Momordica balsamina L.) Cooked by Different Culinary Techniques

et al. 2022

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Prior to consumption, African pumpkin leaves (Momordica balsamina L.) are generally cooked. In this study, the effects of common household cooking methods (boiling, steaming, microwaving, stir-frying) on bioactive metabolites, carotenoids, antioxidant activity, antinutrients and inhibitory effects on α-glucosidase and α-amylase activities were examined. A set of 14 bioactive metabolites were identified in raw and cooked African leaves using UPLC-QTOF/MS. The results showed that the four different types of household cooking methods had different effects on the bioactive metabolomics profile of African pumpkin leaves. In comparison to raw leaves and leaves cooked in other methods, the concentrations of six phenolic compounds, rutin, cryptochlorogenic acid (4-caffeoylquinic acid), pseudolaroside A, isorhamnetin 3-O-robinoside, quercetin 3-galactoside, and trans-4-feruloylquinic acid, were highest in stir-fried leaves. Of all household cooking methods tested, stir-frying increased the content of lutein, β-carotene, and zeaxanthin by 60.00%, 146.15%, and 123.51%, respectively. Moreover, stir-frying African pumpkin leaves increased the antioxidant activity (DPPH and ABTS) and the inhibition of α-glucosidase and α-amylase. Compared to all four methods of household cooking, stir-frying reduced the antinutritive compounds compared to raw leaves. This work provides useful information to the consumers on the selection of suitable cooking methods for African pumpkin leaves.

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Section: Resultsmentioning

confidence: 99%

A Comparison of Bioactive Metabolites, Antinutrients, and Bioactivities of African Pumpkin Leaves (Momordica balsamina L.) Cooked by Different Culinary Techniques

et al. 2022

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“…Flavonoids are most often associated with sugar in the conjugated form to be O-glycosides or C-glycosides. They can also exist as aglycones ( 54 ). The glycosides are normally attached to position 3 or 7, with the most common carbohydrates occupying those positions being D-glucose, L-rhamnose, gluco-rhamnose, galactose or arabinose ( 52 ).…”

Section: Natural Flavonoidsmentioning

confidence: 99%

Flavonoids: Classification, Function, and Molecular Mechanisms Involved in Bone Remodelling

et al. 2021

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Flavonoids are polyphenolic compounds spotted in various fruits, vegetables, barks, tea plants, and stems and many more natural commodities. They have a multitude of applications through their anti-inflammatory, anti-oxidative, anti-carcinogenic properties, along with the ability to assist in the stimulation of bone formation. Bone, a rigid connective body tissue made up of cells embedded in a mineralised matrix is maintained by an assemblage of pathways assisting osteoblastogenesis and osteoclastogenesis. These have a significant impact on a plethora of bone diseases. The homeostasis between osteoblast and osteoclast formation decides the integrity and structure of the bone. The flavonoids discussed here are quercetin, kaempferol, icariin, myricetin, naringin, daidzein, luteolin, genistein, hesperidin, apigenin and several other flavonoids. The effects these flavonoids have on the mitogen activated protein kinase (MAPK), nuclear factor kappa β (NF-kβ), Wnt/β-catenin and bone morphogenetic protein 2/SMAD (BMP2/SMAD) signalling pathways, and apoptotic pathways lead to impacts on bone remodelling. In addition, these polyphenols regulate angiogenesis, decrease the levels of inflammatory cytokines and play a crucial role in scavenging reactive oxygen species (ROS). Considering these important effects of flavonoids, they may be regarded as a promising agent in treating bone-related ailments in the future.

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“…The liquid-liquid partition was performed using 3 portions of petroleum ether, followed by 3 portions of chloroform and, finally, 3 portions of ethyl acetate. [17] The purified fraction was corrected to pH 4 using HCl solution 10%, followed by filtration through a funnel with glass wool and cotton. After purification, the extract was resuspended in saline water to obtain the purified flavonoid SAE.…”

Section: Purified Flavonoid Fractionmentioning

confidence: 99%

Anticoagulant Activity of Crude and Phenolic Extracts of Dalbergia ecastaphyllum (L.) Taub. Dried Leaves

Rovetta¹,

Magenta²,

Gascón³

et al. 2021

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Background: Ischemic diseases are one of the leading causes of death worldwide; therefore, the search for new compounds with safer and more effective anticoagulant and antiplatelet action becomes relevant. Objectives: To evaluate the impact of Dalbergia ecastaphyllum (L.) Taub. dry leaf extract, a species of the Fabaceae family that is commonly found in Brazil, on blood coagulation. Materials and Methods: A statistical design of experiments was carried out with selection of five factors and two levels, resulting in 32 different ethanolic raw extracts. The extracts were resuspended in saline and tested in a pool of human blood. The activated partially thromboplastin time (APTT) and the prothrombin time (PT) were analysed in a Thrombolyzer®. Results: Treatment using ethanol 70%, a drug/solvent ratio of 5%, pH 9 and dynamic maceration for 6 hrs resulted in the best combination for the extraction of total phenols, while ethanol 70%, a drug/solvent ratio of 5% and dynamic maceration for 24 hrs showed the best flavonoid extraction, with the pH being inconclusive. On average, all extracts were capable of increasing both APTT and PT, especially those in lower dilutions (1:5); however, the overall efficacy of the extracts is inconclusive. Conclusion: The species under study showed important results in the blood clotting process in vitro, increasing the mean time of APTT and PT, which suggests that Dalbergia ecastaphyllum (L.) Taub. has some potential as a possible drug in the anticoagulant class.

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Isolation and Identification of the Five Novel Flavonoids from Genipa americana Leaves

Cited by 16 publications

References 41 publications

A Comparison of Bioactive Metabolites, Antinutrients, and Bioactivities of African Pumpkin Leaves (Momordica balsamina L.) Cooked by Different Culinary Techniques

A Comparison of Bioactive Metabolites, Antinutrients, and Bioactivities of African Pumpkin Leaves (Momordica balsamina L.) Cooked by Different Culinary Techniques

Flavonoids: Classification, Function, and Molecular Mechanisms Involved in Bone Remodelling

Anticoagulant Activity of Crude and Phenolic Extracts of Dalbergia ecastaphyllum (L.) Taub. Dried Leaves

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