Isolation and Identification of Talaromyces sp. Strain Q2 and Its Biocontrol Mechanisms Involved in the Control of Fusarium Wilt

Tian, Yehan; Zhao, Yang; Fu, Xuesong; Chang, Yu; Gao, Kexiang; Liu, Huixiang

doi:10.3389/fmicb.2021.724842

Cited by 15 publications

(9 citation statements)

References 55 publications

(55 reference statements)

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“…Talaromyces species have numerous bene ts for the plants; they have been used as biocontrol agents for various pathogens (Tian et al 2020(Tian et al , 2021. Our pot experiments also demonstrated that the spore suspension and fermentation broth of T. cystophila GX1 e ciently decreased H. zeae populations in the maize.…”

Section: Greenhouse Experimentssupporting

confidence: 58%

Novel ectophytic nematode antagonistic fungi and its potential application for nematode biocontrol

Kan

Jia

et al. 2022

Preprint

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Dynamic of plant-parasitic nematodes population in soil is closely related to soil microorganisms. Fungi from Heterodera zeae cysts were isolated to explore the natural decline phenomenon of H. zeae population. A polyphasic method was used to identify the nematode-destroying fungal, including phylogenetic analysis of partial ITS, BenA, CaM, and RPB2 gene sequences, as well as macro- and micro-morphological analyses. The nematicidal activity of strain GX1 against H. zeae was assessed in vitro and greenhouse. Our findings revealed that strain GX1 is a novel species of Talaromyces, which named Talaromyces cystophila. It had strong parasitic and lethal effect on the H. zeae cysts with 91.11% parasitism on cysts at 3 d after treatment, and second-stage juveniles (J2s) and eggs inside the cysts appeared dense vacuoles and loss of vitality. The spore suspension and fermentation filtrate of T. cystophila inhibited significantly the hatching of H. zeae eggs and showed potential nematicidal activities on J2s. The hatching inhibition rates of eggs exposed to 1 × 108 cfu/ml spore suspensions or 20% 1-week fermentation filtrate (1-WF) for 15 days were 98.56% and 100%, respectively. The mortality of J2s exposed to 1 × 108 cfu/ml spore suspension reached 100% at 24 h, exposure to 50% 2-WF was 98.65% and 100% at 24 h and 48 h, respectively. Greenhouse experiments revealed that the spore suspension and fermentation broth considerably decreased H. zeae reproduction by 56.17%-78.76%. T. Cystophila is a potential biocontrol strain with parasitic and nematicidal activity, which deserves attention and application.

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Section: Greenhouse Experimentssupporting

confidence: 58%

Novel ectophytic nematode antagonistic fungi and its potential application for nematode biocontrol

Kan

Jia

et al. 2022

Preprint

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“…Among the genera with negative correlation, the bacterial genus Asanoa had the closest correlation (ρ = −0.7934, p < 0.001), whereas the closest fungal genus was Moniliophthora (ρ = −0.7047, p < 0.001). In addition, genera negatively correlated with RLA included many microbes of disease resistance, such as Massilia (ρ = −0.7277, p < 0.001) ( Li et al., 2021a ), Talaromyces (ρ = −0.6398, p < 0.001) ( Tian et al., 2021c ), Burkholderia (ρ = −0.5569, p < 0.01) ( Ahmad et al., 2022 ), etc.…”

Section: Resultsmentioning

confidence: 99%

Rhizosphere microorganisms of Crocus sativus as antagonists against pathogenic Fusarium oxysporum

Zhang

Zhu

et al. 2022

Front. Plant Sci.

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IntroductionSeveral microorganisms in the plant root system, especially in the rhizosphere, have their own compositions and functions. Corm rot is the most severe disease of Crocus sativus, leading to more than 50% mortality in field production.MethodsIn this study, metagenomic sequencing was used to analyze microbial composition and function in the rhizosphere of C. sativus for possible microbial antagonists against pathogenic Fusarium oxysporum.ResultsThe microbial diversity and composition were different in the C. sativus rhizosphere from different habitats. The diversity index (Simpson index) was significantly lower in the C. sativus rhizospheric soil from Chongming (Rs_CM) and degenerative C. sativus rhizospheric soil from Chongming (RsD_CM) than in others. Linear discriminant analysis effect size results showed that differences among habitats were mainly at the order (Burkholderiales, Micrococcales, and Hypocreales) and genus (Oidiodendron and Marssonina) levels. Correlation analysis of the relative lesion area of corm rot showed that Asanoa was the most negatively correlated bacterial genus (ρ = −0.7934, p< 0.001), whereas Moniliophthora was the most negatively correlated fungal genus (ρ = −0.7047, p< 0.001). The relative lesion area result showed that C. sativus from Qiaocheng had the highest resistance, followed by Xiuzhou and Jiande. C. sativus groups with high disease resistance had abundant pathogen resistance genes, such as chitinase and β-1,3-glucanase genes, from rhizosphere microorganisms. Further, 13 bacteria and 19 fungi were isolated from C. sativus rhizosphere soils, and antagonistic activity against pathogenic F. oxysporum was observed on potato dextrose agar medium. In vivo corm experiments confirmed that Trichoderma yunnanense SR38, Talaromyces sp. SR55, Burkholderia gladioli SR379, and Enterobacter sp. SR343 displayed biocontrol activity against corm rot disease, with biocontrol efficiency of 20.26%, 31.37%, 39.22%, and 14.38%, respectively.DiscussionThis study uncovers the differences in the microbial community of rhizosphere soil of C. sativus with different corm rot disease resistance and reveals the role of four rhizospheric microorganisms in providing the host C. sativus with resistance against corm rot. The obtained biocontrol microorganisms can also be used for application research and field management.

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“…These changes in the composition of different taxonomic groups were presumably due to Fusarium invasion. Generally, Lactobacillus , Streptomyces , Bacillus , Pseudomonas , and Microbacterium have the potential to prevent and control Fusarium pathogens [ 40 , 41 , 42 , 43 , 44 , 45 , 46 ]. These changes observed in the microbial composition of strawberry roots, stems, and leaves were presumably due to the plants’ resistance to F. oxysporum invasion and expansion, thus explaining their resistance to wilting.…”

Section: Discussionmentioning

confidence: 99%

Fusarium Wilt Invasion Results in a Strong Impact on Strawberry Microbiomes

Yang,

Zhang,

Qiu

et al. 2023

Plants

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Plant-endophytic microbes affect plant growth, development, nutrition, and resistance to pathogens. However, how endophytic microbial communities change in different strawberry plant compartments after Fusarium pathogen infection has remained elusive. In this study, 16S and internal transcribed spacer rRNA amplicon sequencing were used to systematically investigate changes in the bacterial and fungal diversity and composition in the endophytic compartments (roots, stems, and leaves) of healthy strawberries and strawberries with Fusarium wilt, respectively. The analysis of the diversity, structure, and composition of the bacterial and fungal communities revealed a strong effect of pathogen invasion on the endophytic communities. The bacterial and fungal community diversity was lower in the Fusarium-infected endophytic compartments than in the healthy samples. The relative abundance of certain bacterial and fungal genera also changed after Fusarium wilt infection. The relative abundance of the beneficial bacterial genera Bacillus, Bradyrhizobium, Methylophilus, Sphingobium, Lactobacillus, and Streptomyces, as well as fungal genera Acremonium, Penicillium, Talaromyces, and Trichoderma, were higher in the healthy samples than in the Fusarium wilt samples. The relative abundance of Fusarium in the infected samples was significantly higher than that in the healthy samples, consistent with the field observations and culture isolation results for strawberry wilt. Our findings provide a theoretical basis for the isolation, identification, and control of strawberry wilt disease.

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Isolation and Identification of Talaromyces sp. Strain Q2 and Its Biocontrol Mechanisms Involved in the Control of Fusarium Wilt

Cited by 15 publications

References 55 publications

Novel ectophytic nematode antagonistic fungi and its potential application for nematode biocontrol

Novel ectophytic nematode antagonistic fungi and its potential application for nematode biocontrol

Rhizosphere microorganisms of Crocus sativus as antagonists against pathogenic Fusarium oxysporum

Fusarium Wilt Invasion Results in a Strong Impact on Strawberry Microbiomes

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