Isolation and identification of specific bacteriophages against methicillin-resistant Staphylococcus aureus, extended-spectrum beta-lactamases-producing Escherichia coli, extended-spectrum beta-lactamases-producing Klebsiella pneumoniae, and multidrug-resistant Acinetobacter baumannii
 in vitro

Fanaei, Vahide; Validi, Majid; Zamanzad, Behnam; Karimi, Ali

doi:10.1093/femsle/fnab139

Cited by 8 publications

(5 citation statements)

References 20 publications

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“…One of the most resistant forms of this bacterium is methicillin-resistant strains. 15 Methicillin is used to treat bacterial infections caused by organisms of the genus Staphylococcus. It is active against certain types of staphylococci, which are resistant to penicillin, but ultimately show resistance to methicillin.…”

Section: Discussionmentioning

confidence: 99%

Identification ofStaphylococcus aureusand prevalence ofStaphylococcus aureusresistant to mithicillin and other patterns of resistance to antibiotics in clinical isolates of diabetic foot

Ramon,

Gonzalez,

Almeida

2024

Preprint

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ObjectiveTo determine the prevalence of methicillin-resistantStaphylococcus aureus(MRSA) in diabetic foot wounds and analyze antibiotic resistance patterns.IntroductionThe global incidence of diabetes mellitus, particularly type II, has significantly increased, leading to complications such as diabetic foot ulcers. These ulcers often become infected, with MRSA being a common and challenging pathogen. Understanding the prevalence and resistance patterns of MRSA in diabetic foot infections is crucial for effective treatment.MethodologyDiabetic foot wound samples were collected from 65 patients in the Valley of Toluca, Mexico. Samples were cultured and analyzed using chromogenic agar, BHI, salt and mannitol, calf blood, EMB, and MacConkey agars. Strain identification and antibiotic sensitivity testing were performed using the Vytek automated system. Oxacillin and cefoxitin were used to detect methicillin resistance. Statistical analyses, including Kolmogorov-Smirnov, Shapiro-Wilk tests, and Spearman correlation, were conducted to evaluate relationships between clinical factors and antibiotic resistance.ResultsOf the 65 samples, 56.9% were from men and 43.1% from women, with 97.1% having type II diabetes. MRSA prevalence was 63%. Resistance rates were highest for ampicillin (100%), clindamycin (89%), erythromycin (87%), and gentamicin (73%). Statistical analysis showed no significant correlation between oxacillin resistance and glycemic control, erythromycin resistance (MLS resistance), hypertension, or gender.ConclusionThe high prevalence of MRSA in diabetic foot wounds underscores the need for targeted infection control and appropriate antibiotic therapy. The lack of correlation between resistance and clinical factors suggests a multifactorial nature of antibiotic resistance, necessitating broader clinical and microbiological considerations for future studies.

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Section: Discussionmentioning

confidence: 99%

Identification ofStaphylococcus aureusand prevalence ofStaphylococcus aureusresistant to mithicillin and other patterns of resistance to antibiotics in clinical isolates of diabetic foot

Ramon,

Gonzalez,

Almeida

2024

Preprint

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“…At present, transcriptomic analyses of A. baumannii phages and their hosts are very scarce. Although many reports have verified the antibacterial potential of phages in vitro or in vivo, there are no data to analyze the changes in phages and bacteria globally [ 162 , 163 , 164 , 165 , 166 , 167 , 168 , 169 ]. This brings a huge workload to the clinical formulation of phage therapy or phage-antibiotic combination therapy.…”

Section: Action Of a Baumannii Phage On ...mentioning

confidence: 99%

Acinetobacter Baumannii Phages: Past, Present and Future

et al. 2023

Viruses

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Acinetobacter baumannii (A. baumannii) is one of the most common clinical pathogens and a typical multi-drug resistant (MDR) bacterium. With the increase of drug-resistant A. baumannii infections, it is urgent to find some new treatment strategies, such as phage therapy. In this paper, we described the different drug resistances of A. baumannii and some basic properties of A. baumannii phages, analyzed the interaction between phages and their hosts, and focused on A. baumannii phage therapies. Finally, we discussed the chance and challenge of phage therapy. This paper aims to provide a more comprehensive understanding of A. baumannii phages and theoretical support for the clinical application of A. baumannii phages.

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“…The most common bacterial species isolated from patients in a COVID-19 ICU are Gram-negative isolates such as A. baumannii and K. pneumoniae , while high proportions of MRSA and VRE were observed among the Gram-positive isolates [ 65 , 66 , 67 ]. Numerous reports indicate that multidrug-resistant K. pneumoniae is one of the most important infectious agents isolated from COVID-19 ICU patients [ 68 , 69 ]. In these patients, co-infection with this pathogen causes a wide range of diseases, including pneumonia, urinary tract infections, bloodstream infections, and sepsis [ 69 ].…”

Section: Introductionmentioning

confidence: 99%

The Dark Side of Nosocomial Infections in Critically Ill COVID-19 Patients

Biondo,

Ponzo,

Midiri

et al. 2023

Life

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Coronavirus disease 2019 (COVID-19) is a potentially serious acute respiratory infection caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). Since the World Health Organization (WHO) declared COVID-19 a global pandemic, the virus has spread to more than 200 countries with more than 500 million cases and more than 6 million deaths reported globally. It has long been known that viral respiratory tract infections predispose patients to bacterial infections and that these co-infections often have an unfavourable clinical outcome. Moreover, nosocomial infections, also known as healthcare-associated infections (HAIs), are those infections that are absent at the time of admission and acquired after hospitalization. However, the impact of coinfections or secondary infections on the progression of COVID-19 disease and its lethal outcome is still debated. The aim of this review was to assess the literature on the incidence of bacterial co-infections and superinfections in patients with COVID-19. The review also highlights the importance of the rational use of antibiotics in patients with COVID-19 and the need to implement antimicrobial stewardship principles to prevent the transmission of drug-resistant organisms in healthcare settings. Finally, alternative antimicrobial agents to counter the emergence of multidrug-resistant bacteria causing healthcare-associated infections in COVID-19 patients will also be discussed.

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Isolation and identification of specific bacteriophages against methicillin-resistant Staphylococcus aureus, extended-spectrum beta-lactamases-producing Escherichia coli, extended-spectrum beta-lactamases-producing Klebsiella pneumoniae, and multidrug-resistant Acinetobacter baumannii in vitro

Cited by 8 publications

References 20 publications

Identification ofStaphylococcus aureusand prevalence ofStaphylococcus aureusresistant to mithicillin and other patterns of resistance to antibiotics in clinical isolates of diabetic foot

Identification ofStaphylococcus aureusand prevalence ofStaphylococcus aureusresistant to mithicillin and other patterns of resistance to antibiotics in clinical isolates of diabetic foot

Acinetobacter Baumannii Phages: Past, Present and Future

The Dark Side of Nosocomial Infections in Critically Ill COVID-19 Patients

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