Isolation and identification of Rhizoctonia-like fungi from roots of three orchid genera, Paphiopedilum, Dendrobium, and Cymbidium, collected in Chiang Rai and Chiang Mai provinces of Thailand

Nontachaiyapoom, Sureeporn; Sasirat, Sawitree; Manoch, Leka

doi:10.1007/s00572-010-0297-3

Cited by 71 publications

(33 citation statements)

References 36 publications

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“…were recorded as mostly belonging to the tulasnelloid fungi (Goh et al 1992;Roberts 1999;Nontachaiyapoom et al 2010).…”

Section: Discussionmentioning

confidence: 99%

“…For comparative analyses of the mycorrhizal fungi sequences from D. chrysanthum and D. nobile, 5.8S and nrLSU alignments, including the closest BLAST matches together with the fungal sequences from mature plants of tropical epiphytes (Suárez et al 2006(Suárez et al , 2008Nontachaiyapoom et al 2010), were analyzed. Sequences were aligned using Clustal X 1.83 (Thompson et al 1997).…”

Section: Phylogenetic Analysesmentioning

confidence: 99%

“…As the seeds of orchids are minute and contain limited energy reserves, their colonization by a compatible fungus is essential for germination under natural conditions (Dearnaley 2007). The potential applications of mycorrhizal association have recently drawn great attention from both horticulturists and conservationists (Nontachaiyapoom et al 2010;Zettler et al 2007;Rasmussen 2002). However, there still remain major issues regarding how to select the adaptable mycorrhizal fungi to ensure successful commercial cultivation.…”

Section: Introductionmentioning

confidence: 99%

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Isolation and identification of endophytic and mycorrhizal fungi from seeds and roots of Dendrobium (Orchidaceae)

2011

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The seed germination of orchids under natural conditions requires association with mycorrhizal fungi. Dendrobium nobile and Dendrobium chrysanthum are threatened orchid species in China where they are considered medicinal plants. For conservation and application of Dendrobium using symbiosis technology, we isolated culturable endophytic and mycorrhizal fungi colonized in the protocorms and adult roots of two species plants and identified them by morphological and molecular analyses (5.8S and nrLSU). Of the 127 endophytic fungi isolated, 11 Rhizoctonia-like strains were identified as Tulasnellales (three strains from protocorms of D. nobile), Sebacinales (three strains from roots of D. nobile and two strains from protocorms of D. chrysanthum) and Cantharellales (three strains from roots of D. nobile), respectively. In addition, species of Xylaria, Fusarium, Trichoderma, Colletotrichum, Pestalotiopsis, and Phomopsis were the predominant non-mycorrhizal fungi isolated, and their probable ecological roles in the Dendrobium plants are discussed. These fungal resources will be of great importance for the large-scale cultivation of Dendrobium plants using symbiotic germination technology and for the screening of bioactive metabolites from them in the future.

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“…were recorded as mostly belonging to the tulasnelloid fungi (Goh et al 1992;Roberts 1999;Nontachaiyapoom et al 2010).…”

Section: Discussionmentioning

confidence: 99%

Section: Phylogenetic Analysesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Isolation and identification of endophytic and mycorrhizal fungi from seeds and roots of Dendrobium (Orchidaceae)

2011

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“…The colonization patterns of ORMs in Central Japan appear to be specific for Sebacina sp., a mycobiont in Cephalanthera falcata but not in C. erecta, which is associated with thelephoroids (Matsuda et al 2009). Sequence homologies of 5.8S rDNAs from Epulorhiza calendulina, isolated from Paphiopedilum species in Thailand (Nontachaiyapoom et al 2010), were related to Sebacina. Kottke and Suárez Chacón (2009) found Sebacina in the Andean Sobralia rosea (Fig.…”

Section: Mycobionts Of Adult Terrestrial Orchidsmentioning

confidence: 99%

Enigmatic Sebacinales

Oberwinkler¹,

Riess²,

Bauer³

et al. 2013

Mycol Progress

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A historical retrospect and a taxonomic update will deal with Sebacina s.l. and s.str., Craterocolla, Efibulobasidium, Serendipita, Trem ell odendron, Trem ell oscypha, Tremellostereum, and Piriformospora, the Sebacinaceae, and the Sebacinales. Phylogenetic hypotheses for the order and subordinal taxa are discussed, including environmental sequence taxa. The cryptic biodiversity in Sebacinales is extensive but mostly unresolved with respect to the species involved.

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“…Bayesian posterior probabilities (PP) were calculated from the majority rule consensus of the tree. The sequence of Septobasidium carestianum (GenBank accession number DQ241448) was selected as outgroup [21].…”

Section: Methodsmentioning

confidence: 99%

Identity and Specificity of Rhizoctonia-Like Fungi from Different Populations of Liparis japonica (Orchidaceae) in Northeast China

et al. 2014

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Mycorrhizal association is known to be important to orchid species, and a complete understanding of the fungi that form mycorrhizas is required for orchid ecology and conservation. Liparis japonica (Orchidaceae) is a widespread terrestrial photosynthetic orchid in Northeast China. Previously, we found the genetic diversity of this species has been reduced recent years due to habitat destruction and fragmentation, but little was known about the relationship between this orchid species and the mycorrhizal fungi. The Rhizoctonia-like fungi are the commonly accepted mycorrhizal fungi associated with orchids. In this study, the distribution, diversity and specificity of culturable Rhizoctonia-like fungi associated with L. japonica species were investigated from seven populations in Northeast China. Among the 201 endophytic fungal isolates obtained, 86 Rhizoctonia-like fungi were identified based on morphological characters and molecular methods, and the ITS sequences and phylogenetic analysis revealed that all these Rhizoctonia-like fungi fell in the same main clade and were closely related to those of Tulasnella calospora species group. These findings indicated the high mycorrhizal specificity existed in L. japonica species regardless of habitats at least in Northeast China. Our results also supported the wide distribution of this fungal partner, and implied that the decline of L. japonica in Northeast China did not result from high mycorrhizal specificity. Using culture-dependent technology, these mycorrhizal fungal isolates might be important sources for the further utilizing in orchids conservation.

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Isolation and identification of Rhizoctonia-like fungi from roots of three orchid genera, Paphiopedilum, Dendrobium, and Cymbidium, collected in Chiang Rai and Chiang Mai provinces of Thailand

Cited by 71 publications

References 36 publications

Isolation and identification of endophytic and mycorrhizal fungi from seeds and roots of Dendrobium (Orchidaceae)

Isolation and identification of endophytic and mycorrhizal fungi from seeds and roots of Dendrobium (Orchidaceae)

Enigmatic Sebacinales

Identity and Specificity of Rhizoctonia-Like Fungi from Different Populations of Liparis japonica (Orchidaceae) in Northeast China

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