Isolation and Identification of protease producing Aeromonas hydrophila

Abd-alla, Maeis T.; Jasim, Hameed M.; Hussein, Ahmed

doi:10.22401/jnus.17.1.19

Cited by 1 publication

(2 citation statements)

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“…Inside other investigation, it found that 100% of A. hydrophila detach can create protease with various degrees. Which in concurrence with our study (25). In the present investigation A. hydrophila identified in 40% of bovine raw milk.…”

Section: Discussionsupporting

confidence: 94%

“…In studies which concurrence with current outcomes, found that psychotrophic populace cooled at 5-10ºC enhanced, especially more than at 0-5 ºC. This outcomes in concurrence with the investigation by Rasolofo et al (27) that discovered psychotrophic populaces expanded inside 72h when refrigerated at 8ºC not at 4ºC,and that the elements of microscopic organisms indicated huge variety for samples from cow, buffalo, and goat (27,25).…”

Section: Discussionsupporting

confidence: 85%

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Genetic detection to Aeromonas hydrophila proteolytic activity in milk samples (cows, buffaloes and goats) in Basra governorate

Banay¹

2020

IJVS

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This study aim is to determine the incidence and the virulence of Aeromonas hydrophila in raw milk, randomly collected from Basra governorate by using of polymerase chain reaction (PCR) technique. In this study, the total number of raw milk samples collected from cows, buffaloes and goats that kept from different the regions of Basra governorate were 90 samples. The PCR technique is modern method which regarded as a reliable tool to detect virulent gene of the A. hydrophila isolates. The PCR assays using the primers sets SerAh-F and SerAh-R resulted in the amplification of 650-bp bands from the targeted proteases gene of the A. hydrophil. The result of the present study showed that the results of PCR concerning the proteolytic activity of A. hydrophila in the tested raw milk samples according to animals' source. The higher percentage of the proteolytic activity was found in the cow's raw milk samples 40% and in the buffalo's milk samples was 26.7% while, the proteolytic activity did not find in the goat's milk samples. The association between the source of the milk sample and proteolytic A. hydrophila positive results was considered to be statistically highly significant. The higher percentage of the A. hydrophila isolates found in the raw cow milk was 40%, and the A. hydrophila isolates found in the raw buffalo milk was 26.7%, while, the A. hydrophila isolates did not find in the goat milk.

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Section: Discussionsupporting

confidence: 94%