Isolation and Identification of Potential Probiotic Bacteria from Human Milk

Damaceno, Quésia Souza; Gallotti, Bruno; Reis, Isabela M M; Totte, Yasmim C P; Assis, Gabriella B. N.; Figueiredo, Henrique César Pereira; Silva, Tales F; Azevedo, Vasco; Nicoli, Jacques R.; Martins, Flaviano S.

doi:10.1007/s12602-021-09866-5

Cited by 14 publications

(8 citation statements)

References 36 publications

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“…In contrast, the auto-aggregation abilities of Lactobacillus gasseri, Lacticaseibacillus rhamnosus, and Limosilactobacillus reuteri were in a variable range between 5.8 and 28.5%, depending on the incubation time and strain [31]. Haemolytic effects were not observed in the tested isolates, and our results agreed with those reported by Damaceno et al (2021) [34].…”

Section: Discussionsupporting

confidence: 91%

Novel Lactic Acid Bacteria Strains from Regional Peppers with Health-Promoting Potential

Nuñez,

Verni,

Argañaraz Martinez

et al. 2024

Fermentation

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This study provides a comprehensive investigation of lactic acid bacteria (LAB) isolated from Argentinean Capsicum annum L. This research covers important aspects, including genotypic characterization, bacterial stress tolerance, adhesion ability, safety evaluation, and functional and technological properties. The predominant isolates were identified as Lactilactobacillus curvatus and Lactiplantibacillus plantarum. A Rep-PCR analysis grouped the isolates into 11 clonal groups. Lp. plantarum LVP 40 and LV 46, Levilactobacillus brevis LVP 41, Pediococcus pentosaceus LV P43, and Lt. curvatus LVP44 displayed both safety and resilience against adverse conditions such as a slow pH, bile, and simulated gastric and intestinal juices. Moreover, the LAB strains exhibited high hydrophobicity and auto-aggregation percentages, NaCl tolerance, and a substantial acidifying capacity. LAB supernatants demonstrated promising surfactant and emulsifying properties. Likewise, they differentially inhibited Staphylococcus aureus and Pseudomonas aeruginosa biofilms, showcasing their potential as antipathogenic agents. Noteworthily, some strains displayed considerable co-aggregation with these pathogens, and several isolates showed an effective antimutagenic and detoxifying power, further emphasizing their multifaceted capabilities. Five pepper bacterial strains showcased beneficial properties, suggesting their potential for gut health enhancement. In summary, these LAB strains hold promise as vegetable fermentation starters, contributing to food safety and versatile applications in food science.

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Section: Discussionsupporting

confidence: 91%

Novel Lactic Acid Bacteria Strains from Regional Peppers with Health-Promoting Potential

Nuñez,

Verni,

Argañaraz Martinez

et al. 2024

Fermentation

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“…Breast milk was evidenced to include a diverse bacterial community that could formulate the infant’s microbiome [ 23 ]. The inclusion of more than 200 bacterial species makes breast milk a good source of natural probiotics [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

“…This reported low count of isolated bacteria is in agreement with another study that mentioned that due to low microbial DNA amounts or sterility, 35% of breast milk from healthy postpartum women could not be isolated or even sequenced. In addition, systemic antibiotic administration to almost all Egyptian mothers after delivery might have reduced microbial count and diversity in HC samples [ 23 ]. The sterility of most HC isolated from Egyptian mothers is attributed to inappropriate use of antibiotics after delivery, as the misuse of antibiotics leads to the destruction of microbiota in addition to the emergence of antibiotic resistance [ 36 , 37 ].…”

Section: Discussionmentioning

confidence: 99%

“…The resultant colonies were initially identified by their morphology on MRS agar plates, Gram staining, and catalase reaction. Catalase-negative isolates were species identified using API 50 CH carbohydrate fermentation strips [ 22 ] (bioMerieux, Craponne, France) and 16S ribosomal ribonucleic acid 16S rRNA [ 23 ]. Following the isolates’ genomic DNA extraction using NucliSENS easyMAG (bioMerieux), 16S rRNA genes were PCR amplified.…”

Section: Methodsmentioning

confidence: 99%

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Evaluation of the Cariogenic and Anti-Cariogenic Potential of Human Colostrum and Colostrum-Derived Probiotics: Impact on S. mutans Growth, Biofilm Formation, and L. rhamnosus Growth

Zaghloul,

Hashem,

El-Sayed

et al. 2023

Life

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Human colostrum (HC) is essential for oral health as it is rich in probiotics that could affect the growth of the cariogenic S. mutans and its biofilm formation; hindering dental caries in advance. In this study, HC was collected from 36 healthy mothers 1–3 days postpartum. The effect of HC on oral health was carried out by assessing the impact of HC and its derived probiotics’ cell-free supernatants (CFS) on the growth of S. mutans (using modified well diffusion) and its biofilm formation (using microtiter plate assay). Moreover, the effect of whole HC on L. rhamnosus, a probiotic oral bacterium, was examined. Probiotics were isolated and identified phenotypically by API 50 CH carbohydrate fermentation and genotypically by 16S rRNA amplification. The in vitro study revealed that HC has cariogenic activity and is associated with biofilm formation. Biofilm strength was inversely proportional to HC dilution (p-value < 0.0001). Nevertheless, HC and colostrum-derived probiotics improve oral health by inhibiting the growth of caries-inducing S. mutans with lower inhibition to L. rhamnosus probiotics. The CFS of isolated probiotics reduced the biofilm formation via the cariogenic S. mutans. These results are not only promising for caries eradication, but they also highlight the importance of breastfeeding infants from their first hours to shape healthy oral microbiota, protecting them from various diseases including dental caries.

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“…To fulfil this requirement, those strains have been isolated from animal or human GIT samples and stool to ensure effectiveness in colonization. Probiotic strains have been isolated even from human milk, which was associated with a good health condition of infants [ 30 ]. Although, there is no restriction of isolation source if the strain is not virulent or pathogenic [ 6 ].…”

Section: Probiotics: the Current State Of An Ancient Practicementioning

confidence: 99%

Journey of the Probiotic Bacteria: Survival of the Fittest

et al. 2022

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This review aims to bring a more general view of the technological and biological challenges regarding production and use of probiotic bacteria in promoting human health. After a brief description of the current concepts, the challenges for the production at an industrial level are presented from the physiology of the central metabolism to the ability to face the main forms of stress in the industrial process. Once produced, these cells are processed to be commercialized in suspension or dried forms or added to food matrices. At this stage, the maintenance of cell viability and vitality is of paramount for the quality of the product. Powder products requires the development of strategies that ensure the integrity of components and cellular functions that allow complete recovery of cells at the time of consumption. Finally, once consumed, probiotic cells must face a very powerful set of physicochemical mechanisms within the body, which include enzymes, antibacterial molecules and sudden changes in pH. Understanding the action of these agents and the induction of cellular tolerance mechanisms is fundamental for the selection of increasingly efficient strains in order to survive from production to colonization of the intestinal tract and to promote the desired health benefits.

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Isolation and Identification of Potential Probiotic Bacteria from Human Milk

Cited by 14 publications

References 36 publications

Novel Lactic Acid Bacteria Strains from Regional Peppers with Health-Promoting Potential

Novel Lactic Acid Bacteria Strains from Regional Peppers with Health-Promoting Potential

Evaluation of the Cariogenic and Anti-Cariogenic Potential of Human Colostrum and Colostrum-Derived Probiotics: Impact on S. mutans Growth, Biofilm Formation, and L. rhamnosus Growth

Journey of the Probiotic Bacteria: Survival of the Fittest

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