Isolation and identification of polyhydroxyalkanoates producing bacteria from biopolymers waste in soil

Yasin, Akram R.; Al-Mayaly, Ithar Kamil

doi:10.1088/1757-899x/928/6/062014

Cited by 5 publications

(5 citation statements)

References 10 publications

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“…Streaked isolates on the PM medium agar (glucose) plates were examined for PHA production by using Sudan B Black staining (Merck, Germany) as described by Kumar et al [2018], smears were examined using light microscope (Olympus, Japan) under (100×) oil immersion objective. Another specific stain method was conducted using Nile Blue A stain (Merck, Germany) which was performed according to Legat et al [2010], all slides were examined at 460 nm using fluorescence microscope (ZEISS AXIOLAB, GERMA-NY) [Yasin and Al-Mayaly, 2020].…”

Section: Screening Of Pha Producing Bacteriamentioning

confidence: 99%

Study of The Fermentation Conditions of the Bacillus Cereus Strain ARY73 to Produce Polyhydroxyalkanoate from Glucose

Yasin¹,

Al-Mayaly²

2021

J. Ecol. Eng.

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Polyhydroxyalkanoates (PHAs) have gained much attention as biodegradable polymers, many efforts are being made to minimize the cost of PHAs by finding cheap carbon source depending on the type of microorganism and fermentation conditions. The aims of this study were to evaluate the effects of different glucose concentrations and other important conditions on the PHA production by Bacillus cereus isolated from soil. Polyhydroxyalkanoates PHAs accumulated by soil microorganisms were examined by screening the isolated bacteria using Sudan B Black and Nile Blue staining process. A Gram positive strain was identified using the 16s rRNA gene, deposited in the NCBI GenBank sequence database. Different growth conditions (favorite glucose concentrations 1-8 % (w/v), temperatures and pH) were tested and the growth parameters (sugar consumption, cell counting and Cell Dry Weight CDW) were studied. The extracted polymers were analyzed and characterized using an FTIR spectrophotometer followed by a GC-MS analysis. The pure bacterial strain isolated from soil was deposited in the NCBI GenBank database B. cereus strain ARY73, which showed significant black colored granules (or dark blue) using Sudan B Black stain, it also showed positive to Nile blue A as a high indicator stain for PHA accumulation. B. cereus ARY73 showed high production of PHA using (w/v): 2% glucose and 1% nitrogen source at 35 °C and pH7 yields 79% per Cell Dry Weight and 96 h of incubation. The extracted polymers were analyzed and characterized using an FTIR spectrophotometer confirming the PHA structure. The FTIR spectrophotometer, followed by a GC-MS analysis indicated the Scl-co-mcl PHA structure. This research demonstrates that the isolated strain B. cereus ARY73 was a good candidate for PHA production with a better quality for use in biomedical and other applications. The use of biopolymer in soil, enhanced the accumulation of the microorganisms (such as bacteria) capable of degrading biopolymer or biodegradation by-products yields by other species which were isolated in this study and demonstrated their efficiency in producing biopolymers.

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Section: Screening Of Pha Producing Bacteriamentioning

confidence: 99%

Study of The Fermentation Conditions of the Bacillus Cereus Strain ARY73 to Produce Polyhydroxyalkanoate from Glucose

Yasin¹,

Al-Mayaly²

2021

J. Ecol. Eng.

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“…As well as spores stained in black color while the cells stained in red color in slide stain method Figure 4. Yasin & Al-Mayaly,2020 [31] had the same finding, showing granules loaded with black staining, as biopolymer granules can be seen as dark spots under the light microscope.…”

Section: Sudan Black Stainmentioning

confidence: 65%

Screening of extracellular polymeric substance producing bacteria isolated from environmental sources and testing their ability to seal fractures zones of oil reservoir rocks

Hamzah¹,

Al-Tamimi²

2022

Proceedings of 2nd International Multi-Disciplinary Conference Theme: Integrated Sciences and Technologies, IMDC-IST 2021, 7-9

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In this study, 21 environmental bacterial isolates isolated from different environmental sources in Basra Governorate identified phenotypically and genetically based on 16 rRNA gene sequence analysis were screened to reveal their ability to produce biopolymer based on the viscosity scale formed in the cultural media and use of Sudan Black stain. Six isolates showed their ability to produce biopolymer, and the two isolates of Bacillus licheniformis strain CC91, Bacillus thuringiensis strain LU3 recorded the best results. The isolate of the Bacillus licheniformis strain CC91 was tested in the process of sealing fractures and openings with high permeability for samples of oil reservoir rocks.

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“…The isolates were subjected to Gram staining followed by biochemical characterization using a VITEK 2 compact system (BioMerieux, France) (Yasin & Al‐Mayaly, 2020). The VITEK 2 system utilizes 63 different tests to conduct generic‐level identification of the bacterial isolates.…”

Section: Methodsmentioning

confidence: 99%

“…The isolates were subjected to Gram staining followed by biochemical characterization using a VITEK 2 compact system (BioMerieux, France) (Yasin & Al-Mayaly, 2020). The VITEK 2 system utilizes 63 were interpreted using the ID-GPC database, and the corresponding identification results were obtained (Ligozzi et al, 2002).…”

Section: Biochemical Characterizationmentioning

confidence: 99%

Evaluation of antagonistic activity of DAPG‐producing fluorescent pseudomonads from rhizospheres of Solanaceous crops for biocontrol of bacterial wilt

Suansia,

Senapati,

Samantaray

et al. 2024

Journal of Phytopathology

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Pseudomonads produce a wide array of antimicrobial compounds including 2,4‐diacetylphloroglucinol (DAPG), which possess a broad‐spectrum antagonistic effect against phytopathogens. Rhizospheric soil samples were collected from the Solanaceous crops for isolation of fluorescent pseudomonads (FPs). Out of the 32 fluorescent bacterial isolates, 21 showed antagonistic activity against Ralstonia solanacearum (NAIMCC‐B‐01630) with the highest zone of inhibition at 38.33 mm. Among them, 11 bacterial isolates depicted the production of DAPG in Kings' B medium. DAPG production was detected by TLC and quantified through HPLC analysis. Moreover, the bacterial isolate OD13 exhibited higher DAPG production. Further, the presence of the DAPG‐encoding phlD gene within the size of 629 bp and 725 bp was also validated through amplification. These bacterial isolates belonged to the genera Pseudomonas as confirmed by morpho‐physiological characterization using the VITEK 2 system. The higher DAPG‐producing bacterial isolate was identified as Pseudomonas aeruginosa by polyphasic approach with GenBank accession number OQ781265. Thus, the DAPG‐producing rhizospheric Pseudomonas strains can be used as biocontrol agents for bacterial wilt. However, field study is highly essential before its successful application.

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Isolation and identification of polyhydroxyalkanoates producing bacteria from biopolymers waste in soil

Cited by 5 publications

References 10 publications

Study of The Fermentation Conditions of the Bacillus Cereus Strain ARY73 to Produce Polyhydroxyalkanoate from Glucose

Study of The Fermentation Conditions of the Bacillus Cereus Strain ARY73 to Produce Polyhydroxyalkanoate from Glucose

Screening of extracellular polymeric substance producing bacteria isolated from environmental sources and testing their ability to seal fractures zones of oil reservoir rocks

Evaluation of antagonistic activity of DAPG‐producing fluorescent pseudomonads from rhizospheres of Solanaceous crops for biocontrol of bacterial wilt

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