Isolation and identification of phase I metabolites of phillyrin in rats

Li, Chang; Yao, Zhihong; Qin, Z. H.; Zhang, Jinbo; Cao, Ruiyuan; Dai, Yi; Yao, Xin‐Sheng

doi:10.1016/j.fitote.2014.05.011

Cited by 23 publications

(15 citation statements)

References 16 publications

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“…Several papers have reported the isolation, purification, and analysis of phillyrin [10][11][12][13][14][15][16][17]. Ultrasonic-assisted extraction (UAE), heat reflux extraction (HRE), decoction extraction (DE), and microwave-assisted extraction (MAE) had been applied to extract the active compounds from F. suspense [10][11][12].…”

Section: Introductionmentioning

confidence: 99%

“…Ultrasonic-assisted extraction (UAE), heat reflux extraction (HRE), decoction extraction (DE), and microwave-assisted extraction (MAE) had been applied to extract the active compounds from F. suspense [10][11][12]. However, methods based on liquid phase separation techniques (LC and LC-MS) had often been used to analyse the phillyrin in F. suspense and its related preparations [13][14][15][16][17][18][19]. Electron ionization-mass spectrometry (EI-MS) has been used to analyze different kinds of compounds, but it is more suitable for a single analyte [20].…”

Section: Introductionmentioning

confidence: 99%

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Comprehensive characterization and quantification of phillyrin in the fruits ofForsythia suspensaand its medicinal preparations by liquid chromatography-ion trap mass spectrometry

Chen

et al. 2016

Acta Chromatographica

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Summary.A rapid and sensitive method for the identification and quantification of phillyrin (POG) in Forsythia suspense is described. The phillyrin standard solution was directly infused into the ion trap mass spectrometers (IT-MS) for collecting the MS n spectra. The electrospray ionization (ESI) mass spectral fragmentation pathway of phillyrin was proposed, and the ESI-MS n fragmentation behavior of phillyrin was deduced in detail. The major product ion at m/z 355 belongs to furofuran, which was formed by loss the glucopyranoside (180 Da), and the characteristic fragment ions m/z 473, 395, 337, 309, and 249 were observed. The loss of 18 Da could arise from two different fragmentation pathways, and the observed ion was composed of a mixture of two different structural ions. Quantification of phillyrin was assigned in positive-ion mode at a product ion at m/z 557 → 355 by liquid chromatography-mass spectrometry (LC-MS). The LC-MS method was validated for linearity, sensitivity, accuracy, and precision and then used to determine the content of the phillyrin. Lastly, the LC-MS method was successfully applied to determine phillyrin in real sample F. suspense and three of its medicinal preparations in the positive mode at the first time.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comprehensive characterization and quantification of phillyrin in the fruits ofForsythia suspensaand its medicinal preparations by liquid chromatography-ion trap mass spectrometry

Chen

et al. 2016

Acta Chromatographica

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“…2). All the spectral data of 10 including the 1D-and 2D-NMR spectra were similar to those of (7R,8R,7′S,8′R)-3′,4-dihydroxy-3,4′,5′-trimethoxy-7,9′,7′,9-diepoxylignane (M4), 18) except the absolute configuration. The nuclear Overhauser effect spectroscopy (NOESY) spectrum showed cross-peaks of H-7 (H-7′) with H-9 ax (H-9′ ax ) and H-8 (H-8′) with H-9 eq (H-9′ eq ) which indicated that the protons were on the same orientations, respectively (Fig.…”

Section: Highlighted Paper Selected By Editor-in-chiefmentioning

confidence: 59%

“…3). The CD spectrum of M4 showed the Cotton effects at 226 nm (Δε+0.72) and 291 nm (Δε−0.20) 18) which suggested a stereoisomer of 10. The CD spectrum of 10 was compared with that of 8 {(7S,8R,7′S,8′R)-configuration}.…”

Section: Highlighted Paper Selected By Editor-in-chiefmentioning

confidence: 99%

Four New Lignans and IL-2 Inhibitors from Magnoliae Flos

et al. 2017

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“…In this study, M1 and M3 were characterized using a UPLC/QTOFMS method, but the modified sites of hydroxylation and dehydrogenation could not be exactly elucidated. Of course, direct isolation of metabolites from biological fluids of human or animals is the most reliable method for identification of metabolites . Thus, the bulk isolation and further structural determination of M1 and M3 remain our ongoing goals.…”

Section: Discussionmentioning

confidence: 99%