Isolation and identification of new microalgae strains with antibacterial activity on food-borne pathogens. Engineering approach to optimize synthesis of desired metabolites

Schuelter, Adílson Ricken; Kroumov, Alexander Dimitrov; Hinterholz, Camila Larissa; Fiorini, Adriana; Trigueros, Daniela Estelita Góes; Vendruscolo, Eliane Gruska; Zaharieva, Maya M.; Módenes, Aparecido Nivaldo

doi:10.1016/j.bej.2019.01.007

Cited by 34 publications

(10 citation statements)

References 57 publications

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“…The metabolic activity of the bacteria after treatment with the MIC concentrations was between 1 and 16%, which points to an bacteriostatic effect. Our data are in accordance with the results published by Schuelter et al [7]. Surprisingly, the addition of low concentrations of the extracts (0.005-0.025 mg/mL) strongly potentiated the antibacterial activity of three clinically used antibacterial drugs-PEN, CIP and ENR -by lowering their effective concentrations two-fold (additive effect) or four-fold (synergism).…”

Section: Discussionsupporting

confidence: 93%

“…Samples of 50 mL withdrawn from PBRs were centrifuged for 10 min at 4000× g, harvested and dried at 105 • C until a constant weight was reached. The harvested biomass was freeze-dried (lyophilized) in a vertical freeze dryer (BIOBASE Group, BK-FD18P, Jinan, Shandong, China) according to the protocol published before [7] and the manufacturer instructions for the freeze dryer usage. Briefly, the microalgal biomass was first frozen at −80 • C within the lyophilizer, and then the samples were placed on shelves in a standard chamber under a deep vacuum and dried for 48 h.…”

Section: Dry Weight Estimation Calculation Of Specific Growth Rate Ly...mentioning

confidence: 99%

“…The microalgae biorefinery concept involves an effective hybrid methodology [6] because the use of biomass from algae as a single technology is a very expensive approach. The application of this concept in every step of the microalgae process development and unit operations by using the principle of analogy and minimizing scientific efforts and costs and finding the cheapest substrates is published in our previous works [7][8][9] where system analysis theory and modelling approach [10][11][12] guided us. Hence, the integral use of all microalgae products such as proteins, lipids, carbohydrates and high-value products (HVP) (e.g., carotenoids, astaxanthin, antimicrobials, antivirals and antifungals products, etc.)…”

Section: Introductionmentioning

confidence: 99%

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Antimicrobial and Antioxidant Potential of Scenedesmus obliquus Microalgae in the Context of Integral Biorefinery Concept

et al. 2022

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Small-scale photobioreactors (PBRs) in the inoculum stage were designed with internal (red or green) and external white LED light as an initial step of a larger-scale installation aimed at fulfilling the integral biorefinery concept for maximum utilization of microalgal biomass in a multifunctional laboratory. The specific growth rate of Scenedesmus obliquus (Turpin) Kützing biomass for given cultural conditions was analyzed by using MAPLE software. For the determination of total polyphenols, flavonoids, chlorophyll “a” and “b”, carotenoids and lipids, UHPLC-HRMS, ISO-20776/1, ISO-10993-5 and CUPRAC tests were carried out. Under red light growing, a higher content of polyphenols was found, while the green light favoured the flavonoid accumulation in the biomass. Chlorophylls, carotenoids and lipids were in the same order of magnitude in both samples. The dichloromethane extracts obtained from the biomass of each PBR synergistically potentiated at low concentrations (0.01–0.05 mg/mL) the antibacterial activity of penicillin, fluoroquinolones or oregano essential oil against the selected food-borne pathogens (Staphylococcus aureus, Escherichia coli and Salmonella typhimurium) without showing any in vitro cytotoxicity. Both extracts exhibited good cupric ion-reducing antioxidant capacity at concentrations above 0.042–0.08 mg/mL. The UHPLC-HRMS analysis revealed that both extracts contained long chain fatty acids and carotenoids thus explaining their antibacterial and antioxidant potential. The applied engineering approach showed a great potential to modify microalgae metabolism for the synthesis of target compounds by S. obliquus with capacity for the development of health-promoting nutraceuticals for poultry farming.

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Section: Discussionsupporting

confidence: 93%

Section: Dry Weight Estimation Calculation Of Specific Growth Rate Ly...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Antimicrobial and Antioxidant Potential of Scenedesmus obliquus Microalgae in the Context of Integral Biorefinery Concept

et al. 2022

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“…Previous studies suggested that modifying the culture conditions of green microalgae exhibited differences in antibacterial activity [47][48][49], whereas modifications on microalgae culture conditions could be stimulated to produce secondary metabolites with antibacterial activity, as well as potentially larger quantities of these secondary metabolites [50]. This may include modifications on media composition, pH, light, and temperature [51,52]. As an example, the highest light intensity (4800 lux) exposure effectively induced the production of antibacterial compounds by Dunaliella sp.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Antibacterial Activity of Marine Microalgae Extract against Vibrio harveyi

et al. 2022

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Marine microalgae may produce antibacterial substances. At the exponential phase of growth, four species of marine microalgae were examined for their potential to create secondary metabolites that limit the growth of Vibrio harveyi: Nannochloropsis oceanica, Chaetoceros gracilis, Isochrysis sp., and Thalassiosira weissflogii. V. harveyi is a pathogenic bacteria that can cause severe mortality and loss in aquaculture. Disc diffusion assay and co-culture assay were used to determine antibacterial activity. On TSA % NaCl media, the disc impregnated with microalgae and extracted with ethanol, methanol, saline water, and dimethyl sulfoxide (DMSO) was tested against V. harveyi at concentrations of 1.0 × 105, 106 and 107 CFU mL−1. The disc diffusion assay revealed that N. oceanica extracted with ethanol had the largest inhibitory zone against V. harveyi. Meanwhile, only N. oceanica, Isochrysis sp., and T. weissflogii reduced the growth of V. harveyi (105 CFU mL−1) in the co-culture assay (p < 0.05). The current findings reveal that the hydrophilic chemicals in microalgae extract have antibiotic activity against the highly virulent V. harveyi, which causes vibriosis, a serious disease in farmed fish and aquaculture cultivation around the world.

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“…Extracts from microalgal biomass habour antimicrobial properties and have been documented to inhibit and kill potentially food spoilage and human pathogenic microorganisms such as bacteria, fungi and viruses (Rizwan et al, 2018;Schuelter et al, 2019). The effectiveness of the microalgal extract is highly dependent on the type of microalga, the solvent used for extraction and the concentration of the extract (Pina-Pérez et al, 2017).…”

Section: Antimicrobial Applicationsmentioning

confidence: 99%

Biotechnological Applications of Microalgal Oleaginous Compounds: Current Trends on Microalgal Bioprocessing of Products

et al. 2020

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The microalgal biotechnology industry is expanding rapidly and currently gaining attention due to multiple availabilities of high-value products such as pigments, carbohydrates, proteins, nutraceuticals, biopharmaceuticals, and unique oleaginous compounds fractionated by biomass biorefinery. Microalgae are efficient primary producers in the terrestrial and marine biotopes. They are major sources of global oxygen and are gaining topical prominence due to their concomitant role in the phycoremediation of wastewater effluents and biomass production. Despite their minuscule size, microalgae critically contribute to climate change mitigation through carbon fixation and play a major role in bioenergy applications. Furthermore, carotenoids and phycobiliproteins are the main accessory light-harvesting complexes in microalgae and cyanobacteria. The topical biomedical and pharmaceutical applications of microalgae include anticancer, antidiabetic, antiHIV, antimalarial, antimicrobial, inter alia. The endowment of unique indigenous microalgae and utilization of these biological resources must be harnessed by the biorefinery industry to exploit microalgal biomass opportunities. Therefore, this manuscript factually and critically explores the current status of the biorefinery approach, topical biomedical and pharmaceutical applications, biofuel applications, genetic manipulation of microalgae for enhancement of product yield, challenges and presents prospects, pros and cons, and outlook of the microalgal biotechnology industry.

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Isolation and identification of new microalgae strains with antibacterial activity on food-borne pathogens. Engineering approach to optimize synthesis of desired metabolites

Cited by 34 publications

References 57 publications

Antimicrobial and Antioxidant Potential of Scenedesmus obliquus Microalgae in the Context of Integral Biorefinery Concept

Antimicrobial and Antioxidant Potential of Scenedesmus obliquus Microalgae in the Context of Integral Biorefinery Concept

In Vitro Antibacterial Activity of Marine Microalgae Extract against Vibrio harveyi

Biotechnological Applications of Microalgal Oleaginous Compounds: Current Trends on Microalgal Bioprocessing of Products

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