Isolation and Identification of Myxobacteria from Saline-Alkaline Soils in Xinjiang, China

Zhang, Xianjiao; Yao, Qing; Zhang, Cai; Xie, Xianmei; Zhu, Honghui

doi:10.1371/journal.pone.0070466

Cited by 33 publications

(33 citation statements)

References 28 publications

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“…No growth occurred at 44 °C. This is in accordance to Zhang et al (2003) who reported that almost all Myxococcus and Corallococcus strains grew within a temperature range of 10-37°C. Strains K9-5 and V3-1 were sensitive to spectinomycin, chloramphenicol, fusidic acid, oxytetracyclin, thiostrepton, and trimethoprim, but were resistant to ampicillin, gentamycin, kanamycin, cephalosporin, hygromycin, polymycin, and bacitracin.…”

Section: Antimicrobial Potential Of Pure Strainssupporting

confidence: 93%

“…The majority of strains were identified as either Myxococcus or Corallococcus species. A similarly large representation of these two genera was also found by Zhang et al (2003). Classification of the myxobacteria isolates on the basis of morphological features is represented in Table 2.…”

Section: Resultssupporting

confidence: 60%

“…Predatory myxobacteria, which represent the vast majority of myxobacteria, can degrade these microorganisms, form fruiting bodies and even swarm over the agar. On the other hand, baiting with dung pellets had the advantage of developing clear fruiting bodies, although subsequent purification by picking was difficult, because natural rabbit pellets are rich in organic matter and easily contaminated by molds during the induction process, covering the fruiting bodies of myxobacteria (Zhang et al 2003). Taxonomy of the myxobacteria relies mainly on morphological characteristics such as shape and size of vegetative cells, myxospores, and colors and types of swarms, and fruiting bodies (Reichenbach and Dworkin 1992;Spröer et al 1999).…”

Section: Resultsmentioning

confidence: 99%

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Soil myxobacteria as a potential source of polyketide-peptide substances

et al. 2017

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Myxobacteria, a group of antimicrobial producing bacteria, have been successfully cultured and characterized from 10 soil samples collected from different parts of Slovakia. A total of 79 myxobacteria belonging to 4 genera (Myxococcus, Corallococcus, Sorangium, and Polyangium) were isolated based aspects on of their life cycle. Twenty five of them were purified, fermented, and screened for antimicrobial activities against 11 test microorganisms. Results indicated that crude extracts showed more significant activities against Gram-positive than against Gram-negative bacteria or fungi. Based on a higher degree and broader range of antimicrobial production, the two most potential extracts (K9-5, V3-1) were selected for HPLC fractionation against Micrococcus luteus and Staphylococcus aureus and LC/MS analysis of potential antibiotic metabolites. The analysis resulted in the identification of polyketide-peptide antibiotics, namely corallopyronin A and B (K9-5) and myxalamid B and C (V3-1), which were responsible for important Gram-positive activity in the observed strains. A sequence similarity search through BLAST revealed that these strains showed the highest sequence similarity to Corallococcus coralloides (K9-5, NCBI accession number KX256198) and Myxococcus xanthus (V3-1, NCBI accession number KX256197). Although screening of myxobacteria is laborious, due to difficulties in isolating cultures, this research represented the first report covering the isolation and cultivation of this challenging bacterial group from Slovakian soils as well as the screening of their antimicrobial activity, cultural identification, and secondary metabolite identification.

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Section: Antimicrobial Potential Of Pure Strainssupporting

confidence: 93%

Section: Resultssupporting

confidence: 60%

Section: Resultsmentioning

confidence: 99%

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Soil myxobacteria as a potential source of polyketide-peptide substances

et al. 2017

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“…1. Myxobacteria taxonomy was earlier associated only with morphology, which is adequate in differentiating between genera [30]. Nowadays, the identification of myxobacteria up to the species level is commonly derived from 16S rRNA gene analysis.…”

Section: Characterization Of the Most Potent Myxococcus And Corallocomentioning

confidence: 99%

Isolation, antimicrobial activity of myxobacterial crude extracts and identification of the most potent strains

Charousová

Medo

Javoreková

2017

Arch biol sci (Beogr)

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Broad spectrum antimicrobial agents are urgently needed to fight frequently occurring multidrug-resistant pathogens. Myxobacteria have been regarded as "microbe factories" for active secondary metabolites, and therefore, this study was performed to isolate two bacteriolytic genera of myxobacteria, Myxococcus sp. and Corallococcus sp., from 10 soil/sand samples using two conventional methods followed by purification with the aim of determining the antimicrobial activity of methanol extracts against 11 test microorganisms (four Gram-positive, four Gram-negative, two yeasts and one fungus). Out of thirty-nine directly observed strains, 23 were purified and analyzed for antimicrobial activities. Based on the broth microdilution method, a total of 19 crude extracts showed antimicrobial activity. The range of inhibited wells was more important in the case of anti-Gram-positive-bacterial activity in comparison with the anti-Gram-negative-bacterial and antifungal activity. In light of the established degree and range of antimicrobial activity, two of the most active isolates (BNEM1 and SFEC2) were selected for further characterization. Morphological parameters and a sequence similarity search by BLAST revealed that they showed 99% sequence similarity to Myxococcus xanthus − BNEM1 (accession no. KX669224) and Corallococcus coralloides -SFEC2 (accession no. KX669225). As these isolates had antimicrobial activity, they could be considered for use in the development of antibiotics for pharmaceutical use.

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“…According to the explanation for this phenomenon by Reichenbach (19), they are not actually indigenous limnetic organisms, and should originally come from terrestrial surroundings, and are finally transported into water bodies. Surprisingly, myxobacteria have also found in some extreme conditions, such as acidic wetlands (21), saline-alkaline soils (22), the arid desert (23), and even hot springs (24). More unexpectedly, a few myxobacteria have sporadically isolated from marine habitats (25), and different from known terrestrial myxobacteria, particularly in the requirement of sodium chloride roughly equivalent to the salinity of seawater (26).…”

Section: Introductionmentioning

confidence: 99%

Meta-16S rRNA gene phylogenetic reconstruction reveals astonishing diversity of the cosmopolitan myxobacteria

Liu

Yao

Zhu

2019

Preprint

Self Cite

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23Numerous ecological studies for myxobacteria have been conducted well, but their 24 true diversity hidden in plain sight remains to be explored. To bridge this gap, we here 25 implemented a comprehensive survey of diversity and distribution of myxobacteria by 26 using 4997 publicly available 16S rRNA gene sequences (≥1200 bp) collected from 27 several hundreds of sites across multiple countries and regions. In the study, the 28 meta-16S rRNA gene phylogenetic reconstruction clearly revealed that these 29 sequences were classified to 998 species, 445 genera, 58 families, and 20 suborders, 30 highlighting a considerable taxonomic diversity of myxobacteria, the great majority of 31 which belonged to new taxa. Most cultured myxobacteria including the 32 well-described type strains were strongly inclined to locate on the shallow branches of 33 the phylogenetic tree; on the contrary, the majority of uncultured myxobacteria the 34 deep branches. The geographical analysis of sequences based on their environmental 35 categories clearly demonstrated that myxobacteria showed a nearly cosmopolitan 36 distribution, despite the presence of some habitat-specific taxa, especially at genus 37 and species levels. Among all abundant suborders, members of Suborder_4, 38 Suborder_15, and Suborder_17 were more widely distributed in marine environments, 39 the remaining suborders preferred to reside in terrestrial ecosystems, particularly in 40 soils, indicating a potential selectivity of geographical distribution. In conclusion, this 41 study profiles a clear framework of diversity and distribution of the cosmopolitan 42 myxobacteria and sheds light on the isolation of the uncultured myxobacteria. 43 44 48thousands of publicly available and high-quality 16S rRNA gene sequences of 49 myxobacteria by using the phylogenetic reconstruction. The study presented an 50 astonishing diversity than that expected from the previous studies. This study further 51 demonstrated that the culturability of myxobacteria was perfectly comparable to its 52 phylogeny in the phylogenetic tree. The geographical analysis clearly indicated that 53 myxobacteria showed a nearly cosmopolitan distribution, while some taxa exhibited 54 obvious preferences for specific environmental conditions. Together, our study 55 provides novel insights into the diversity, distributions, and ecological preferences of 56 of myxobacteria from diverse environments and lays the foundation for innovation of 57 isolation techniques and the discovery of new secondary metabolites. 58 59 KRYWORDS: myxobacteria; phylogenetic diversity; geographical distribution; 60 environments 61 62 63 64 65 66 67 Myxobacteria are a large group of organisms, which taxonomically belong to the 68 order Myxococcales within the class Deltaproteobacteria (1). They are one of nature's 69 "endowed" and extensively dispersed in natural environments, preferentially in places 70 that are rich in microbes and organic matter. Myxobacteria are one of the fascinating 71 and unique bacteria by virtue of thei...

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Isolation and Identification of Myxobacteria from Saline-Alkaline Soils in Xinjiang, China

Cited by 33 publications

References 28 publications

Soil myxobacteria as a potential source of polyketide-peptide substances

Soil myxobacteria as a potential source of polyketide-peptide substances

Isolation, antimicrobial activity of myxobacterial crude extracts and identification of the most potent strains

Meta-16S rRNA gene phylogenetic reconstruction reveals astonishing diversity of the cosmopolitan myxobacteria

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