Isolation and identification of Mycoplasma capricolum subsp. capripneumoniae from goats

Abstract

Contagious caprine pleuropneumonia is a transmissible disease in goats caused by Mycoplasma capricolum subsp. capripneumoniae (Mccp). The disease is responsible for remarkable morbidity and mortality in goats. In Pakistan, data regarding its prevalence and incidence is lacking. This research study was aimed at providing the current prevalence rate in Punjab, Pakistan. For this, 50 samples including pleural fluids, nasal swabs and trachea were collected from slaughterhouses, livestock markets and veterinary hospitals around Faisalabad and processed for the determination of Contagious caprine pleuropneumonia by culture and confirmation by polymerase chain reaction. Characteristics fried egg colonies and Giemsa staining were indicative of Mccp. The culture positive samples were then processed to detect 16S rRNA gene of Mccp. Based on cultural and molecular evidence: a) The prevalence of Contagious caprine pleuropneumonia was 40% in slaughterhouse specimens, b) The prevalence of contagious caprine pleuropneumonia was 46.66% in livestock markets and, c) The prevalence of contagious caprine pleuropneumonia was 15% in veterinary hospitals.