Isolation and Identification of Mushroom Pathogens from<i>Agrocybe aegerita</i>

Choi, In‐Young; Choi, Jang-Nam; Sharma, Praveen Kumar; Lee, Wang-Hyu

doi:10.4489/myco.2010.38.4.310

Cited by 26 publications

(16 citation statements)

References 17 publications

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“…This study demonstrated that Ageritin is able to exert inhibitory action only against the T. asperellum although the protein concentration able to inhibit 50% of fungal growth was about 40-fold higher than that required for P. digitatum growth inhibition [ 34 ]. These findings are in agreement with the defense role hypothesized for Ageritin, also considering that during cultivation, edible mushrooms could be contaminated by several ascomycetes fungi, such as Trichoderma species, that cause fungal fruiting bodies damage by inhibiting its growth [ 66 ]. RNAs from Ageritin-treated P. digitatum were also isolated and analyzed confirming the release of α-fragment mediated by Ageritin in vivo [ 52 ].…”

Section: Antiproliferative and Defense Activities Of Ageritinsupporting

confidence: 87%

Ageritin from Pioppino Mushroom: The Prototype of Ribotoxin-Like Proteins, a Novel Family of Specific Ribonucleases in Edible Mushrooms

Ragucci

Russo

Valletta

et al. 2021

Toxins

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Ageritin is a specific ribonuclease, extracted from the edible mushroom Cyclocybe aegerita (synonym Agrocybe aegerita), which cleaves a single phosphodiester bond located within the universally conserved alpha-sarcin loop (SRL) of 23–28S rRNAs. This cleavage leads to the inhibition of protein biosynthesis, followed by cellular death through apoptosis. The structural and enzymatic properties show that Ageritin is the prototype of a novel specific ribonucleases family named ‘ribotoxin-like proteins’, recently found in fruiting bodies of other edible basidiomycetes mushrooms (e.g., Ostreatin from Pleurotus ostreatus, Edulitins from Boletus edulis, and Gambositin from Calocybe gambosa). Although the putative role of this toxin, present in high amount in fruiting body (>2.5 mg per 100 g) of C. aegerita, is unknown, its antifungal and insecticidal action strongly support a role in defense mechanisms. Thus, in this review, we focus on structural, biological, antipathogenic, and enzymatic characteristics of this ribotoxin-like protein. We also highlight its biological relevance and potential biotechnological applications in agriculture as a bio-pesticide and in biomedicine as a therapeutic and diagnostic agent.

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Section: Antiproliferative and Defense Activities Of Ageritinsupporting

confidence: 87%

Ageritin from Pioppino Mushroom: The Prototype of Ribotoxin-Like Proteins, a Novel Family of Specific Ribonucleases in Edible Mushrooms

Ragucci

Russo

Valletta

et al. 2021

Toxins

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“…Indirect positive effects of bacteria on the PM were tested by measuring the influence of bacteria on the growth of molds ( Fig 4 ; S2 Table ). Molds are frequently detected from fungal fruiting bodies [ 16 , 18 , 53 ] and some molds are known as pathogens [ 54 – 56 ] or early decomposers of dead fruiting bodies [ 57 ]. Because of the diversity of enzyme activities among the molds ( Table 1 ), it is likely that the presence of molds often introduces some negative effects on the maintenance of the PM fruiting body.…”

Section: Discussionmentioning

confidence: 99%

Effect of fruiting body bacteria on the growth of Tricholoma matsutake and its related molds

Kim

Eimes

et al. 2018

PLoS ONE

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Tricholoma matsutake (pine mushroom, PM) is a prized mushroom in Asia due to its unique flavor and pine aroma. The fruiting body of PM forms only in its natural habitat (pine forest), and little is known regarding the natural conditions required for successful generation of the fruiting bodies in this species. Recent studies suggest that microbial interactions may be associated with the growth of PM; however, there have been few studies of the bacterial effects on PM growth. In this study, we surveyed which bacteria can directly and indirectly promote the growth of PM by using co-cultures with PM and molds associated with the fruiting body. Among 16 bacterial species isolated from the fruiting body, some species significantly influenced the mycelial growth of PM and molds. Most bacteria negatively affected PM growth and exhibited various enzyme activities, which suggests that they use the fruiting body as nutrient source. However, growth-promoting bacteria belonging to the Dietzia, Ewingella, Pseudomonas, Paenibacillus, and Rodococcus were also found. In addition, many bacteria suppressed molds, which suggests an indirect positive effect on PM as a biocontrol agent. Our results provide important insights toward a better understanding of the microbial interactions in the fruiting body of PM, and indicate that growth-promoting bacteria may be an important component in successful cultivation of PM.

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“…The process requires practical exclusion of other contaminant microorganisms, involving strict hygiene conditions that are generally too costly and impractical to operate on a large scale especially in low income countries. Unfortunately, any failure to achieve a satisfactory harvest may often lead to heavy loss in mushroom production (Choi et al, 2010;Bellettini et al, 2016;Chang and Wasser, 2017). The contaminants exhibit fast growth and usually out-compete the mycelia of mushrooms for space and nutrients.…”

Section: Introductionmentioning

confidence: 99%

Sensitivity of mycelial growth of mushroom Agrocybe semiorbicularis to different concentrations of different disinfectants

Buba¹,

Agbo²,

Abdullahi³

et al. 2019

bta

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This study was performed to determine the effects of different types of disinfectants (Hypo, Izal, and Dettol) on the mycelial growth of the mushroom Agrocybe semiorbicularis. The more evolutionarily advanced mushroom mycelium was expected to show greater resistance to disinfectants than other fungal and bacterial contaminants. Minimal disinfectant concentration was the one at which contaminants were inhibited, while the growth of the desired mushroom mycelia remained unaffected. Different concentrations of different disinfectants were added to the growth media, and the pure mushroom mycelial culture was inoculated on the media and left to grow. The results revealed that the probability of contamination was higher in all the concentrations of Hypo and in lower concentrations of Dettol and Izal. At 5% concentration of the disinfectants (Hypo, Izal, and Dettol), the mean values of contamination were 0.667, 0.417, and 0.00 (P < 0.001), respectively. At 10% concentration, the mean contamination values were 0.167, 0.583, and 0.333 (P > 0.05), respectively, while at 15% concentration, the mean contamination values were 1.000, 0.417, and 0.250 (P < 0.05), respectively. At higher concentrations of the disinfectants, the growth of contaminants was completely suppressed, and the growth of the desired mycelia was also significantly decreased. At 17% concentration of the disinfectants (Hypo, Izal, and Dettol), the mean values of contamination were 0.833, 0.833, and 0.00 (P < 0.05), respectively. At 18% disinfectant concentration, the mean contamination values were 0.167, 0.00, and 0.00 (P > 0.05), respectively, while at 20% disinfectant concentration, the mean contamination values were 0.583, 0.00, and 0.00(P < 0.05), respectively. The mean values of the mushroom's mycelial growth for the three disinfectants (

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Isolation and Identification of Mushroom Pathogens fromAgrocybe aegerita

Cited by 26 publications

References 17 publications

Ageritin from Pioppino Mushroom: The Prototype of Ribotoxin-Like Proteins, a Novel Family of Specific Ribonucleases in Edible Mushrooms

Ageritin from Pioppino Mushroom: The Prototype of Ribotoxin-Like Proteins, a Novel Family of Specific Ribonucleases in Edible Mushrooms

Effect of fruiting body bacteria on the growth of Tricholoma matsutake and its related molds

Sensitivity of mycelial growth of mushroom Agrocybe semiorbicularis to different concentrations of different disinfectants

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