Isolation and identification of mucin-degrading bacteria originated from human faeces and their potential probiotic efficacy according to host–microbiome enterotype

Hu, Yuan; Zhou, Junyu; Li, Nanxin; Wu, Xuangao; Huang, Shaokai; Park, Sunmin

doi:10.1111/jam.15560

Cited by 4 publications

(3 citation statements)

References 47 publications

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“…Co-culture of Lactobacillus and mucin has been reported to promote the colonization of Lactobacillus in a dynamic gut model and also increase their resistance to antibiotics ( 52 – 54 ). Another report confirmed that Lactobacillus could reduce the colonization of pathogenic bacteria in the intestinal mucosa through competitive adhesion ( 55 , 56 ). Recently, whether mucin promoted the enrichment of L. mucosae or L. mucosae utilizing mucin as a functional substrate remains unclear.…”

Section: Discussionmentioning

confidence: 84%

Mucin alleviates colonic barrier dysfunction by promoting spermine accumulation through enhanced arginine metabolism in Limosilactobacillus mucosae

Zhou,

Xu,

Zhang

et al. 2024

mSystems

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Dietary fiber deprivation is linked to probiotic extinction, mucus barrier dysbiosis, and the overgrowth of mucin-degrading bacteria. However, whether and how mucin could rescue fiber deprivation-induced intestinal barrier defects remains largely unexplored. Here, we sought to investigate the potential role and mechanism by which exogenous mucin maintains the gut barrier function. The results showed that dietary mucin alleviated fiber deprivation-induced disruption of colonic barrier integrity and reduced spermine production in vivo . Importantly, we highlighted that microbial-derived spermine production, but not host-produced spermine, increased significantly after mucin supplementation, with a positive association with upgraded colonic Lactobacillus abundance. After employing an in vitro model, the microbial-derived spermine was consistently dominated by both mucin and Lactobacillus spp. Furthermore, Limosilactobacillus mucosae was identified as an essential spermine-producing Lactobacillus spp., and this isolated strain was responsible for spermine accumulation, especially after adhering to mucin in vitro . Specifically, the mucin-supplemented bacterial supernatant of Limosilactobacillus mucosae was verified to promote intestinal barrier functions through the increased spermine production with a dependence on enhanced arginine metabolism. Overall, these findings collectively provide evidence that mucin-modulated microbial arginine metabolism bridged the interplay between microbes and gut barrier function, illustrating possible implications for host gut health. IMPORTANCE Microbial metabolites like short-chain fatty acids produced by dietary fiber fermentation have been demonstrated to have beneficial effects on intestinal health. However, it is essential to acknowledge that certain amino acids entering the colon can be metabolized by microorganisms to produce polyamines. The polyamines can promote the renewal of intestinal epithelial cell and maintain host-microbe homeostasis. Our study highlighted the specific enrichment by mucin on promoting the arginine metabolism in Limosilactobacillus mucosae to produce spermine, suggesting that microbial-derived polyamines support a significant enhancement on the goblet cell proliferation and barrier function.

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Section: Discussionmentioning

confidence: 84%

Mucin alleviates colonic barrier dysfunction by promoting spermine accumulation through enhanced arginine metabolism in Limosilactobacillus mucosae

Zhou,

Xu,

Zhang

et al. 2024

mSystems

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“…Meanwhile, g_Lactobacillus has antibacterial activity, can inhibit pathogenic bacteria, reduce intestinal inflammatory response and protect intestinal epithelial cells and mucous membranes (Eor et al, 2019). Additionally, g_Prevotellaceae_UCG.001 could promote intestinal peristalsis by SCFAs production, thereby increasing the water content in feces due to the degradation of mucus proteins and by changing ion transmembrane transport in the intestinal lumen (Yuan et al, 2022). Acupuncture may modulate the intestinal environment in multiple ways, such as reducing oxidative stress (Zhang et al, 2022), regulating immunity, reducing inflammation (Yang et al, 2021), and thereby increase the abundance of g_Lactobacillus and g_ Prevotellaceae_UCG.001.…”

Section: Acupuncture Alters the Composition Of Gut Microbiota In Pati...mentioning

confidence: 99%

Effects of acupuncture on gut microbiota and short-chain fatty acids in patients with functional constipation: a randomized placebo-controlled trial

Yan,

Yao,

et al. 2023

Front. Pharmacol.

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Objective: To comprehensively evaluate the effect of acupuncture on gut microbiota, identify specific microbes closely related to the clinical efficacy of acupuncture, and explored the role of short-chain fatty acids (SCFAs).Methods: A randomized placebo-controlled trial was conducted with 80 FC patients and 28 healthy controls (HCs). FC patients randomly received 16 acupuncture (n = 40) or sham acupuncture (n = 40) sessions over 4 weeks; HCs received no treatment. The change in the proportion of patients with mean weekly complete spontaneous bowel movements (CSBMs) was considered as the primary outcome measure. Moreover, the composition and the predictive metabolic function of the gut microbiota from feceal samples were analyzed by 16S rRNA gene sequencing, while feceal SCFAs were identified via gas chromatography-mass spectrometry (GC-MS).Results: Compared to sham acupuncture, acupuncture significantly increased the proportion of CSBM responders, and improved spontaneous bowel movements (SBMs), straining, stool consistency, and quality of life. Moreover, Sequencing of 16S rRNA genes revealed that acupuncture improved β-diversity and restored the composition of gut microbiota. Specifically, the abundance of beneficial bacteria such as g_Lactobacillus increased while that of pathogenic bacteria such as g_Pseudomonas decreased after acupuncture, which were significantly correlated with alleviated symptoms. Moreover, ten microbes including g_Coprobacter, g_Lactobacillus, and g_Eubacterium_coprostanoligenes_group might be considered acupuncture-specific microbes, and formed a stable interaction network. Additionally, GC-MS analysis indicated that acupuncture increased the content of butyrate acid in the gut, which was positively correlated with an increase in defecation frequency and a decrease in acupuncture-related pathogens. Finally, acupuncture specific-microbes including g_Coprobacter, g_Lactobacillus, g_Pseudomonas, g_Eubacterium_coprostanoligenes_group, g_Erysipelotrichaceae_UCG.003, g_Prevotellaceae_UCG.001, and g_Rolstonia could accurately predict the clinical efficacy of acupuncture (AUC = 0.918).Conclusion: Acupuncture could effectively improve clinical symptoms in FC patients, and was associated with gut microbiota reshaping and increased butyrate acid levels. Moreover, key microbial genera such as g_Coprobacter and g_Lactobacillus was predictive of acupuncture efficacy in treating FC. Future studies are required to validate the causal relationship between key microbial genera and acupuncture clinical efficacy, and should explore further metabolic pathways for designing personalized treatment strategies.Clinical Trial Registration:http://www.chictr.org.cn, Identifier: ChiCTR2100048831.

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“…Some bacterial species, within the normal gut microbiota composition, display mucin-degrading features and, at the same time, do the fermentation of nutritional fibers. This leads to short-chain fatty acids (SCFA) production, which are vital nutrients and energy sources for the colon epithelium ( 54 ). Gut dysbiosis reduces butyrate and acetate levels, depriving the epithelium of needed nutrients, which can cause epithelium dysfunction ( 55 – 57 ).…”

Section: Ssri and Ibdmentioning

confidence: 99%

Selective serotonin reuptake inhibitors and inflammatory bowel disease; Beneficial or malpractice

et al. 2022

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IBD, a chronic inflammatory disease, has been manifested as a growing health problem. No Crohn’s and Colitis councils have officially ratified anti-depressants as a routine regimen for IBD patients. However, some physicians empirically prescribe them to rectify functional bowel consequences such as pain and alleviate psychiatric comorbidities. On the other side, SSRIs’ prescription is accompanied by adverse effects such as sleep disturbances. Prolonged intermittent hypoxia throughout sleep disturbance such as sleep apnea provokes periodic reductions in the partial oxygen pressure gradient in the gut lumen. It promotes gut microbiota to dysbiosis, which induces intestinal inflammation. This phenomenon and evidence representing the higher amount of serotonin associated with Crohn’s disease challenged our previous knowledge. Can SSRIs worsen the IBD course? Evidence answered the question with the claim on anti-inflammatory properties (central and peripheral) of SSRIs and illuminated the other substantial elements (compared to serotonin elevation) responsible for IBD pathogenesis. However, later clinical evidence was not all in favor of the benefits of SSRIs. Hence, in this review, the molecular mechanisms and clinical evidence are scrutinized and integrated to clarify the interfering molecular mechanism justifying both supporting and disproving clinical evidence. Biphasic dose-dependent serotonin behavior accompanying SSRI shifting function when used up for the long-term can be assumed as the parameters leading to IBD patients’ adverse outcomes. Despite more research being needed to elucidate the effect of SSRI consumption in IBD patients, periodic prescriptions of SSRIs at monthly intervals can be recommended.

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Isolation and identification of mucin-degrading bacteria originated from human faeces and their potential probiotic efficacy according to host–microbiome enterotype

Cited by 4 publications

References 47 publications

Mucin alleviates colonic barrier dysfunction by promoting spermine accumulation through enhanced arginine metabolism in Limosilactobacillus mucosae

Mucin alleviates colonic barrier dysfunction by promoting spermine accumulation through enhanced arginine metabolism in Limosilactobacillus mucosae

Effects of acupuncture on gut microbiota and short-chain fatty acids in patients with functional constipation: a randomized placebo-controlled trial

Selective serotonin reuptake inhibitors and inflammatory bowel disease; Beneficial or malpractice

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