Isolation and identification of mesenchymal stem cells from human lipoma tissue

Lin, Tsai-Ming; Chang, Hong-Po; Wang, Kai-Hung; Kao, An-Pei; Chang, Chia‐Cheng; Wen, Cheng-Hao; Lai, Chung-Sheng; Lin, Sin-Daw

doi:10.1016/j.bbrc.2007.07.116

Cited by 79 publications

(79 citation statements)

References 31 publications

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“…MSCs were initially identified in bone marrow but later have also been isolated from other tissues such as adipose tissue [1], umbilical cord [2], periodontal ligament [3], articular cartilage [4], muscle [5], periosteum [6], and synovium [7]. Recently, MSCs have also been identified in tendons [8][9] and we have shown that tendonderived stem cells (TDSCs) could promote tendon repair in an acute rat patellar tendon window injury model (unpublished results).…”

Section: Introductionmentioning

confidence: 89%

Effect of In Vitro Passaging on the Stem Cell-Related Properties of Tendon-Derived Stem Cells—Implications in Tissue Engineering

Tan

Lui

Rui

2012

Stem Cells and Development

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This study aimed to compare clonogenicity, proliferation, stem cell-related marker expression, senescence, and differentiation potential of rat patellar tendon-derived stem cells (TDSCs) at early (P5), mid (P10), and late (P20, P30) passages. The clonogenicity of the cells was assessed by colony-forming assay and their proliferative potential was assessed by bromodeoxyuridine assay. The surface expression of CD90 and CD73 was assessed by flow cytometry. The cellular senescence was assessed by b-galactosidase activity. The adipogenic, chondrogenic, and osteogenic differentiation potentials of TDSCs were assessed by standard assays after induction. The mRNA expression of tendon-related markers, scleraxis (Scx) and tenomodulin (Tnmd), was measured by quantitative real-time reverse transcription-polymerase chain reaction. Both the colony numbers and proliferative potential of TDSCs increased with passaging. Concomitantly, there was significant upregulation of b-galactosidase activity with TDSC passaging. The subculture of TDSCs downregulated the expression of CD90 and CD73. Lipid droplets were formed in the early and mid passages of TDSCs upon adipogenic induction, but were absent in the late passages. The expression of peroxisome proliferator activator receptor gamma 2 (PPARc2) and CCAAT/ enhancer binding protein alpha (C/EBPa) in TDSCs after adipogenic induction decreased with passaging. Chondrogenesis, proteoglycan deposition, collagen type II protein expression, collagen type 2A1 (Col2AI), and aggrecan (Acan) mRNA expression were less in pellets formed with later passages of TDSCs after chondrogenic induction. The expression of Scx and Tnmd was lower in the late, compared with early and mid, passages of TDSCs. However, matrix mineralization and expression of alkaline phosphatase (Alpl) and osteocalcin (Bglap) mRNA after osteogenic induction increased with TDSC passaging. Researchers and clinicians should consider the changes of stem cell-related properties of TDSCs when multiplying them in vitro for tissue engineering.

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Section: Introductionmentioning

confidence: 89%

Effect of In Vitro Passaging on the Stem Cell-Related Properties of Tendon-Derived Stem Cells—Implications in Tissue Engineering

Tan

Lui

Rui

2012

Stem Cells and Development

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“…Such processes have been implicated in several aspects of epithelial tumor biology, such as tumor growth, neoplastic progression, angiogenesis, and metastasis [6,7]. MSCs have been isolated from different tumor types such as ovarian carcinomas [8], giant cell tumors of bone [9], neuroblastomas [10], osteosarcomas [11], lipomas [12], and gastric cancer [13]; however, the presence of MSCs in cervical cancer (CeCa) and their possible role in such tumor growth have not been documented.…”

Section: Introductionmentioning

confidence: 99%

“…the isolation of MSCs from several tumors [8][9][10][11][12][13]; however, the presence of MSCs has not been demonstrated in NCx or in CeCa tissue. Following this, and taking into consideration the immunossupressive properties of MSCs [35], we reasoned that the presence of these cells in cervical tumors may represent an important immunoselective advantage for tumor cells to evade the immune recognition.…”

mentioning

confidence: 99%

In Vitro Evidence of the Presence of Mesenchymal Stromal Cells in Cervical Cancer and Their Role in Protecting Cancer Cells from Cytotoxic T Cell Activity

Montesinos

Mora‐García²,

Mayani

et al. 2013

Stem Cells and Development

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“…MSC-like cells can be isolated from tumors such as bone sarcomas, lipomas, and gastric cancers [21][22][23][24] . The characteristics of these MSC-like cells, with respect to morphology, gene expression, surface antigen expression, and differentiation potential, are similar to those of BM-derived MSCs (BM-MSCs).…”

Section: Introductionmentioning

confidence: 99%

Bone Marrow-Derived Mesenchymal Stem Cells Regulate Epithelial-Mesenchymal Transitions and Gastric Cancer Stem Cells

Aoyagi

Kizaki

Isobe

et al. 2017

Journal of Gastroenterology and Hepatology Research

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from MSCs. Direct contact between BM-derived MSCs (BMMSCs) and gastric cancer cells in vitro increases the proportion of CD133-positive gastric cancer cells. This mechanism is thought to involve stimulation of Wnt-5a and transforming growth factor (TGF)-β by BM-BMCs, directly affecting the gastric cancer cells. Moreover, direct contact between BM-MSCs and gastric cancer cells increases the expression of vimentin and Snail, which is a transcription factor that mediates epithelial-mesenchymal transitions (EMT). Human BM-MSCs can enhance the proliferation, invasion, and chemoresistance of gastric cancer cells. The regulatory mechanism involved is likely associated with increased expression of stem cell markers and EMT-related factors in gastric cancer cells. CD271, which is a marker of BM-MSCs, is expressed in scirrhous carcinoma, and the expression of Wnt-5a and TGF-β in cancer cells is up-regulated. BM-MSCs provide an advantageous tumor microenvironment for gastric cancer. Moreover, BM-MSC-related molecules may be considered biomarkers of gastric cancer. INTRODUCTIONDuring tumor progression, epithelial-mesenchymal transitions (EMT) contribute considerably to the malignant characteristics of tumors, including local invasion and distant metastasis [1,2] . EMT is the key phenomenon that tightly regulates the stem cell-like characteristics of both normal and malignant cells [3,4] . Since cancer stem cells (CSCs) in solid cancers were first identified in the early half of the 2000s, ABSTRACTTumorigenesis is driven by alterations in tumor cells and also changes in the stromal microenvironment. Mesenchymal stem cells (MSCs) are promising candidates for the treatment of various tumors. MSCs are unlikely to be rejected by the immune system, could potentially home to the tumor site, and may interact with tumor cells to influence their growth and metastasis. Using mouse models of inflammationinduced gastric cancer, at least 20% of cancer-associated fibroblasts were found to originate from bone marrow (BM) and were derived REVIEW

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Isolation and identification of mesenchymal stem cells from human lipoma tissue

Cited by 79 publications

References 31 publications

Effect of In Vitro Passaging on the Stem Cell-Related Properties of Tendon-Derived Stem Cells—Implications in Tissue Engineering

Effect of In Vitro Passaging on the Stem Cell-Related Properties of Tendon-Derived Stem Cells—Implications in Tissue Engineering

In Vitro Evidence of the Presence of Mesenchymal Stromal Cells in Cervical Cancer and Their Role in Protecting Cancer Cells from Cytotoxic T Cell Activity

Bone Marrow-Derived Mesenchymal Stem Cells Regulate Epithelial-Mesenchymal Transitions and Gastric Cancer Stem Cells

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