Isolation and Identification of Major Mastitis Causing Bacteria from Clinical Cases of Bovine Mastitis in Kashmir Valley

Waseem, Rabia; Muhee, Amatul; Malik, H. U.; Akhoon, Z. A.; Munir, Khusheeba; Nabi, SU; Taifa, Syed

doi:10.18805/ijar.b-3848

Cited by 6 publications

(7 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Escherichia coli isolates were tested in Ethiopia and 27 (100%) were MDR, 188 (100%) E. coli isolates in Nigeria were reported to be resistant to three to seven different classes of antibiotics, and 76 (53%) E. coli isolates demonstrated multidrug resistance in Vietnam ( 4 , 7 , 14 ). A discovery of MDR E. coli was made in milk, perhaps because of improper or excessive antibiotic use in treating infectious diseases in dairy cattle, environmental and farm personnel contamination transferred during the milking process, or the free movement of the animal ( 25 ). In this study, only one (0.99%) MDR isolate of E. coli was found to be positive in a DDST.…”

Section: Discussionmentioning

confidence: 99%

Molecular identification of blaTEM and blaCTX-M genes in multidrug-resistant Escherichia coli found in milk samples from dairy cattle farms in Tulungagung, Indonesia

Widodo,

Lamid,

Effendi

et al. 2023

Journal of Veterinary Research

View full text Add to dashboard Cite

Introduction Escherichia coli is an opportunistic bacteria that can grow easily, produce toxins, and resist antibiotics. The phenomenon of E. coli developing multidrug resistance is currently the subject of extensive research. The objective of this study was to molecularly identify blaTEM and blaCTX-M genes in multidrug-resistant E. coli found in milk samples from dairy cattle farms in Tulungagung, Indonesia. Material and Methods One hundred and ten milk samples were collected from 45 dairy cattle farms in Tulungagung, Indonesia. Indole, methyl red, Voges–Proskauer and in citrate tests and triple iron sugar agar tests were used to identify E. coli. Multidrug resistance was determined in isolates through antibiotic sensitivity tests using tetracycline, streptomycin, trimethoprim, chloramphenicol and aztreonam. Extended-spectrum beta lactamase enzyme production was confirmed by double-disc synergy test (DDST). Molecular identification was performed to confirm the blaTEM and blaCTX-M genes. Results One hundred and one (91.82%) E. coli strains were isolated from the samples. The antibiotic sensitivity test showed four (3.96%) multidrug-resistant (MDR) and one (0.99%) ESBL-positive E. coli by DDST confirmation. There were three (77.78%) blaTEM genes and one (0.99%) blaCTX-M gene discovered in the MDR E. coli isolates using PCR for molecular identification. Conclusion The findings of the blaTEM and blaCTX-M genes encoding ESBL E. coli in dairy cattle milk in Tulungagung, Indonesia is concerning and argues for prompt action to stop the emergence of antibiotic resistance which has an impact on public health.

show abstract

Section: Discussionmentioning

confidence: 99%

Molecular identification of blaTEM and blaCTX-M genes in multidrug-resistant Escherichia coli found in milk samples from dairy cattle farms in Tulungagung, Indonesia

Widodo,

Lamid,

Effendi

et al. 2023

Journal of Veterinary Research

View full text Add to dashboard Cite

show abstract

“…Mastitic milk was collected aseptically using sterile vials and subjected to somatic cell counting (SCC). Coliform mastitis was determined through selective media (MacConkey agar and EMB agar) followed by gram staining and biochemical tests: Indole Methyl red Voges-Proskauer Citrate tests (IMViC) [ 38 ].…”

Section: Methodsmentioning

confidence: 99%

Scavenger receptor B1 facilitates the endocytosis of Escherichia coli via TLR4 signaling in mammary gland infection

et al. 2023

View full text Add to dashboard Cite

SCARB1 belongs to class B of Scavenger receptors (SRs) that are known to be involved in binding and endocytosis of various pathogens. SRs have emerging role in regulating innate immunity and host–pathogen interactions by acting in co-ordination with Toll-like receptors.Query Little is known about the function of SCARB1 in milk-derived mammary epithelial cells (MECs). This study reports the role of SCARB1 in infection and its potential association in TLR4 signaling on bacterial challenge in Goat mammary epithelial cells (GMECs). The novelty in the establishment of MEC culture lies in the method that aims to enhance the viability of the cells with intact characteristics upto a higher passage number. We represent MEC culture to be used as a potential infection model for deeper understanding of animal physiology especially around the mammary gland. On E.coli challenge the expression of SCARB1 was significant in induced GMECs at 6 h. Endoribonuclease-esiRNA based silencing of SCARB1 affects the expression of TLR4 and its pathways i.e. MyD88 and TRIF pathways on infection. Knockdown also affected the endocytosis of E.coli in GMECs demonstrating that E.coli uses SCARB1 function to gain entry in cells. Furthermore, we predict 3 unique protein structures of uncharacterized SCARB1 (Capra hircus) protein. Overall, we highlight SCARB1 as a main participant in host defence and its function in antibacterial advances to check mammary gland infections.

show abstract

“…Coliform mastitis was determined through selective media (MacConkey agar and EMB agar) followed by gram staining and biochemical tests: Indole Methyl red Voges-Proskauer Citrate tests (IMViC) (38).…”

Section: Bacterial Identificationmentioning

confidence: 99%

Scavenger receptor B1 facilitates the endocytosis of Escherichia coli via TLR4 signaling in mammary gland infection

Taban

Ahmad

Mumtaz

et al. 2022

Preprint

View full text Add to dashboard Cite

SCARB1 belongs to class B of Scavenger receptors (SRs) that are known to be involved in binding and endocytosis of various pathogens. SRs have emerging role in regulating innate immunity and host-pathogen interactions by acting in co-ordination with Toll-like receptors. Little is known about the function of SCARB1 in milk-derived mammary epithelial cells (MECs). This study reports the role of SCARB1 in infection and its potential association in TLR4 signaling on bacterial challenge in Goat mammary epithelial cells (GMECs). The novelty in the establishment of MEC culture lies in the method that aims to enhance the viability of the cells with intact characteristics upto a higher passage number. We represent MEC culture to be used as a potential infection model for deeper understanding of animal physiology especially around the mammary gland. On E.coli challenge the expression of SCARB1 was significant in induced GMECs at 6 h. Endoribonuclease- esiRNA based silencing of SCARB1 affects the expression of TLR4 and its pathways i.e. MyD88 and TRIF pathways on infection. Knockdown also affected the endocytosis of E.coli in GMECs demonstrating that E.coli uses SCARB1 function to gain entry in cells. Furthermore, we predict 3 unique protein structures of uncharacterized SCARB1 (Capra hircus) protein. Overall, we highlight SCARB1 as a main participant in host defence and its function in antibacterial advances to check mammary gland infections.

show abstract

Isolation and Identification of Major Mastitis Causing Bacteria from Clinical Cases of Bovine Mastitis in Kashmir Valley

Cited by 6 publications

References 8 publications

Molecular identification of blaTEM and blaCTX-M genes in multidrug-resistant Escherichia coli found in milk samples from dairy cattle farms in Tulungagung, Indonesia

Molecular identification of blaTEM and blaCTX-M genes in multidrug-resistant Escherichia coli found in milk samples from dairy cattle farms in Tulungagung, Indonesia

Scavenger receptor B1 facilitates the endocytosis of Escherichia coli via TLR4 signaling in mammary gland infection

Scavenger receptor B1 facilitates the endocytosis of Escherichia coli via TLR4 signaling in mammary gland infection

Contact Info

Product

Resources

About