Isolation and Identification of Listeria monocytogenes from Raw Vegetables and Meat Sold in Quetta, Pakistan

Samad, Abdul; Asmat, Raheela; Ali, Hamida; Mustafa, Mohammad Zahid; Abbas, Ferhat; Raza, Jannat; Asmat, Muhammad Tauseef

doi:10.17582/journal.pjz/20190402110434

Cited by 5 publications

(2 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although RTE food sold by street vendors is cheap in price, its microbial quality may be compromised if proper hygienic measures are not adopted. In developing countries such as Pakistan, such measures are often neglected, and food items have been reported to be contaminated with several pathogens (Samad et al, 2018a(Samad et al, , b, 2019(Samad et al, , 2020. The results of the present study are alarming, as none of the RTE food samples tested (n = 320) were free of bacteria.…”

Section: Discussioncontrasting

confidence: 53%

Contamination of ready-to-eat street food in Pakistan with Salmonella spp.: Implications for consumers and food safety

Raza

Asmat

Mustafa

et al. 2021

International Journal of Infectious Diseases

Self Cite

View full text Add to dashboard Cite

Ready-to-eat (RTE) food sold in Quetta, Pakistan was assessed for microbial contamination. Methods: Equal numbers of samples were collected from four categories of RTE foodburgers, shawarma, pizza and sandwichesfrom January 2018 to December 2018. Microbial contamination of individual food samples was assessed by quantifying the total aerobic count obtained from plating samples on bacterial growth medium. Salmonella spp. serovars were identified using polymerase chain reaction. Results: Approximately 38% (121/320) of RTE food samples were not fit for human consumption. The most contaminated type of RTE food was shawarma (49%). Microbial contamination of food samples was higher in summer compared with the other seasons. Approximately 40% (49/121) of food samples that were not fit for human consumption were contamined with Salmonella spp. Salmonella enteritidis (69%) and Salmonella typhimurium (31%) were the only serovars among the samples testing positive for Salmonella spp. Of the 49 samples with high microbial counts, S. enteritidis was present in 34 samples and S. typhimurium was present in 15 samples. The antibiotic sensitivity results demonstrated that both S. enteritidis and S. typhimurium were resistant to amoxicillin. In addition, S. enteritidis was resistant to chloramphenicol and erythromycin, and S. typhimurium presented high resistance to erythromycin. Both S. typhimurium and S. enteritidis were highly sensitive to kanamycin. Conclusion: RTE food sold by street vendors in Quetta was found to be contaminated with Salmonella spp. and poses a great health risk to consumers. As such, consumption should be avoided, and the health authorities should take stringent action to ensure the quality of street food in order to reduce the healthcare burden.

show abstract

Section: Discussioncontrasting

confidence: 53%

Contamination of ready-to-eat street food in Pakistan with Salmonella spp.: Implications for consumers and food safety

Raza

Asmat

Mustafa

et al. 2021

International Journal of Infectious Diseases

Self Cite

View full text Add to dashboard Cite

show abstract

“…Also, it is believed to have a dual intercellular lifestyle, which helps it to invade, colonise and establish in a host [5]. Common food that can easily be contaminated includes dairy [6], vegetables [7, 8], meat and poultry [9,10]. A recent survey [11] found that serogroup 1/2a-3a dominate food, 4a-4c in livestock, and 1/2a-3a in humans.…”

Section: Introductionmentioning

confidence: 99%

Probing the Evolution of Genes Associated With DNA Methylation inListeria monocytogenes

Nwaiwu,

Onyeaka,

Rees

2023

Preprint

View full text Add to dashboard Cite

In the last decade, there have been increased reports of atypicalListeriaand the discovery of new species. There are public health concerns that new strains may come with increased pathogenicity. Hence, this study aimed to establish the prevalence, evolutionary lineage and ancestry of aListeria monocytogenescollection that includes isolates that harbour a unique set of methylase genes. The addition of methyl groups to DNA can interfere with transcription. Allelic-specific lineage analysis and ribotyping with southern hybridization were carried out after which further phylogenetic analysis was performedin silico. Results show that all the methylase strains belonged to Lineage I and were serotypes 4b or 4d. All designated ancestral strains also belonged to Lineage 1. AListeria monocytogenesplasmid from a serotype 1/2a (Lineage II) contained sequences homologous to that of Lineage I isolates. The methylase nucleotide sequence in the strains studied appears to be highly conserved inListeria monocytogenesand not yet orthologous among other bacterial genera. It is of epidemiological interest and public benefit if wider or continuous surveillance is carried out to ascertain if these rare strains are linked with increased pathogenesis, food type or geographical region.

show abstract

Listeria monocytogenes an Emerging Pathogen: a Comprehensive Overview on Listeriosis, Virulence Determinants, Detection, and Anti-Listerial Interventions

et al. 2023

View full text Add to dashboard Cite

Isolation and Identification of Listeria monocytogenes from Raw Vegetables and Meat Sold in Quetta, Pakistan

Abstract: Authors' Contribution AS, FA and TMA designed the experiments. AS, MN and HA performed the experiments. TMA, JR and RA analyzed the data. All authors contributed in Manuscript writing.

Cited by 5 publications

References 25 publications

Contamination of ready-to-eat street food in Pakistan with Salmonella spp.: Implications for consumers and food safety

Contamination of ready-to-eat street food in Pakistan with Salmonella spp.: Implications for consumers and food safety

Probing the Evolution of Genes Associated With DNA Methylation inListeria monocytogenes

Listeria monocytogenes an Emerging Pathogen: a Comprehensive Overview on Listeriosis, Virulence Determinants, Detection, and Anti-Listerial Interventions

Contact Info

Product

Resources

About