Isolation and identification of<i>Vibrio</i>species in the Rio Bravo/Grande and water bodies from Reynosa, Tamaulipas

Guardiola-Avila, Iliana; Martínez-Vázquez, V.; Requena-Castro, Rocío; Juárez-Rendón, Karina Janett; Aguilera‐Arreola, Ma. Guadalupe; Rivera, Gildardo; Bocanegra-García, Virgilio

doi:10.1111/lam.13009

Cited by 2 publications

(2 citation statements)

References 32 publications

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“…Similarly, Joshi et al [45] reported that none of their Vibrio isolates carrying both tdh and trh genes. A study by Guardiola-Avila et al [7] did not discover the ctxA gene, which is consistent with our findings. In addition, several other researchers have also discovered that this gene may not always present in the environment [58].…”

Section: Discussionsupporting

confidence: 93%

“…Vibrio spp. such as V. alginolyticus, V. anguillarum, V. harveyi, V. vulnificus, and V. parahaemolyticus are the most common species that cause vibriosis in shrimp [7]. Furthermore, V. parahaemolyticus is one of the several causative agents of shrimp disease that has become more prevalent as global shrimp demand and production have increased [8].…”

Section: Introductionmentioning

confidence: 99%

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Characterization of the Virulency of Vibrio spp. in Giant Tiger Shrimp (Penaeus monodon) from Peninsular Malaysia

Haifa-Haryani,

Azzam-Sayuti,

Amatul-Samahah

et al. 2023

Aquaculture Research

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Nowadays, bacterial diseases caused by Vibrio spp. pose a significant obstacle to aquaculture growth, affecting socioeconomic development. The presence of pathogenic Vibrio spp. are gaining attention due to widespread mortality in fish and shellfish worldwide. This study investigates the distribution of virulence genes and their pathogenicity by experimentally infecting a healthy group of cultured shrimp, specifically Penaeus monodon. A total of 225 Vibrio spp. isolated from 210 cultured shrimp from various geographical regions in Peninsular Malaysia were examined, resulting in 13 Vibrio spp. being identified and characterized. According to virulence gene assessment, only eight of the 11 virulence genes were detected in the isolates, including chiA (89%), flaC (74%), tlh (72%), toxR (70%), luxR (40%), pirA (11%), pirB (11%), and hlyA (0.4%). Other virulence genes, such as tdh, trh, and ctxA, were not found in any of the isolates (0%). Then, three Vibrio isolates, including V. parahaemolyticus S12-3, V. campbellii S10-4, and V. rotiferianus S24-4, were selected for pathogenicity test based on highest number of virulence genes in each species. These species were subjected to immersion challenge test against postlarvae (PL) P. monodon approximately PL-20 with three different concentrations of 1 × 106, 1 × 107, and 1 × 108 CFU/mL. The V. parahaemolyticus S12-3 was determined to have lethal concentration 50 (LC50) 1 × 105 CFU/mL after 168 hr of exposure. The immersion challenge test resulted in a pale hepatopancreas and empty guts in P. monodon. Histopathological examination showed sloughing of epithelial cells in the hepatopancreatic tubule, hemocytic infiltration, massive vacuolation, and loss of hepatopancreatic tubule structure. Overall, the study demonstrated the virulence and pathogenicity of V. parahaemolyticus S12-3 toward P. monodon, which could be associated with its virulence properties. Furthermore, the current findings will be useful in developing microbiological risk assessments for shrimp farming and aquaculture management.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%