Isolation and identification of Bacillus megaterium YB3 from an effluent contaminated site efficiently degrades pyrene

Abstract

Industrial effluents contaminated sites may serve as repositories of ecologically adapted efficient pyrene degrading bacteria. In the present study, six bacterial isolates from industrial effluents were purified using serial enrichment technique and their pyrene degrading potential on pyrene supplemented mineral salt medium was assessed. 16S rRNA sequence analysis showed that they belong to four bacterial genera, namely Acinetobacter, Bacillus, Microbacterium, and Ochrobactrum. Among these isolates, Bacillus megaterium YB3 showed considerably good growth and was further evaluated for its pyrene-degrading efficiency. B. megaterium YB3 could degrade 72.44% of 500 mg L(-1) pyrene within 7 days. GC-MS analysis of ethyl acetate extracted fractions detected two relatively less toxic metabolic intermediates of the pyrene degradation pathway. B. megaterium YB3 also tested positive for catechol 1, 2-dioxygenase and aromatic-ring-hydroxylating dioxygenase indole-indigo conversion assays. Considering the ability and efficiency of B. megaterium YB3 to degrade high pyrene content, the strain can be used as a tool to develop bioremediation technologies for the effective biodegradation of pyrene and possibly other PAHs in the environment.

Microbial biodegradation of plastics: Challenges, opportunities, and a critical perspective

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Perspectives of Microbial Inoculation for Sustainable Development and Environmental Management

“…Additionally, JPN2 showed increased growth at 100 mg L −1 of pyrene, indicating its tolerance for pyrene (Jin et al, 2016). Meena et al (2016) isolated pyrene degrading bacteria from industrial effluent contaminated sites which suggest these sites may be attractive niches for PAH degrading bacteria. From the industrial effluent, six bacterial isolates were isolated and purified following serial enrichment techniques, and were assessed for their pyrene degradation potential using modified mineral salt medium supplemented with pyrene.…”