Isolation and Identification of Bacillus amyloliquefaciens IBFCBF‐1 with Potential for Biological Control of Phytophthora Blight and Growth Promotion of Pepper

Abstract

In this study, 76 bacterial strains were isolated from the rhizosphere soil of pepper. Of these, 23 bacterial isolates capable of inhibiting Phytophthora capsici growth were selected. Among the antagonistic bacteria, one strain, IBFCBF‐1 showed the strongest antagonistic activity, and was identified as Bacillus amyloliquefaciens based on the results of 16S rRNA gene sequence analysis, physiological and biochemical testing, and morphological characteristics. When tested with a dual‐culture method and with laboratory greenhouse studies, the strain IBFCBF‐1 was found to be a potential biocontrol agent for controlling the plant pathogen, P. capsici. Moreover, it showed high efficiency and broad‐spectrum antifungal properties in vitro. Under greenhouse conditions, IBFCBF‐1 could significantly promote the growth of pepper seedlings, and was able to solubilize phosphate, and produce indole acetic acid (IAA) and ammonia. This study clearly demonstrated that IBFCBF‐1 is a potential candidate exhibiting phytophthora blight‐suppressive and plant growth‐promoting effects on pepper.

Not Just a Pathogen? Description of a Plant-Beneficial Pseudomonas syringae Strain

“…This increase in height remained consistent until the late stages of growth, the non-treated plants catching up with the growth of the 260-02: gfp treated plants, though there was still a statistically significant difference in height. The increase in height of the treated peppers was around 20%, in line with earlier results obtained with growth promoters (Kang et al, 2007; Zhang et al, 2016).…”

Isolation, Identification, and Antibacterial Mechanisms of Bacillus amyloliquefaciens QSB-6 and Its Effect on Plant Roots

“…), wheat (root rot, leaf blight, etc. ), and other crop diseases ( Baker et al, 1983 ; Wilson and Pusey, 1985 ; Lin et al, 2001 ; Chan et al, 2003 ; Souto et al, 2004 ; Ouhaibi-Ben Abdeljalil et al, 2016 ; Zalila-Kolsi et al, 2016 ; Zhang M. et al, 2016 ; Fan et al, 2017b ; Gómez Expósito et al, 2017 ; Qin et al, 2017 ; Barratt et al, 2018 ; Wang X. Q. et al, 2018 ). Ramírez et al (2021) isolated a B. cereus MH778713 from root nodules of Prosopis laevigata , and the volatile hentriacontane and 2,4-di-tert-butylphenol produced by it can effectively inhibited the radial growth of F. oxysporum and protected tomato plants against Fusarium wilt .…”

“… Mutaz Al-Ajlani and Hasnain (2010) determined that 54 of 118 Bacillus strains isolated from soil samples had antagonistic activities toward at least two strains from a panel of pathogenic and non-pathogenic microorganisms. Zhang M. et al (2016) isolated Bacillus amyloliquefaciens IBFCBF-1 that can effectively control Phytophthora blight and promote the growth of pepper. Singh et al (2008) found that B. subtilis BN1 isolated from the chir pine ( Pinus roxburghii ) rhizosphere exhibited strong antagonistic activity toward F. oxysporum and R. solani .…”

Current Insights into the Role of Rhizosphere Bacteria in Disease Suppressive Soils

“…Following this line of research, several microbial genera have been proposed for their role in specific disease suppressiveness. These include (fluorescent) Pseudomonas ( Kloepper et al, 1980 ; Scher and Baker, 1980 , 1982 ; Wong and Baker, 1984 ; Lemanceau and Alabouvette, 1991 ; Raaijmakers and Weller, 1998 ; De Souza et al, 2003 ; Perneel et al, 2007 ; Mazurier et al, 2009 ; Mendes et al, 2011 ; Michelsen and Stougaard, 2011 ), Streptomyces ( Liu et al, 1996 ; Cha et al, 2016 ), Bacillus ( Sneh et al, 1984 ; Cazorla et al, 2007 ; Abdeljalil et al, 2016 ; Zhang et al, 2016 ), Paenibacillus ( Haggag and Timmusk, 2008 ), Enterobacter ( Schisler and Slininger, 1994 ; Abdeljalil et al, 2016 ), Alcaligenes ( Yuen and Schroth, 1986 ), Pantoea ( Schisler and Slininger, 1994 ), non-pathogenic F. oxysporum ( Sneh et al, 1987 ; Couteaudier and Alabouvette, 1990 ; Larkin et al, 1996 ; Larkin and Fravel, 2002 ; Nel et al, 2006 ; Mazurier et al, 2009 ; Raaijmakers et al, 2009 ), Trichoderma ( Harman et al, 1980 ; Liu and Baker, 1980 ; Chet and Baker, 1981 ; Hadar et al, 1984 ; Smith et al, 1990 ; Mghalu et al, 2007 ), Penicillium janczewskii ( Madi and Katan, 1998 ), V. biguttatum ( Jager and Velvis, 1983 ; Velvis and Jager, 1983 ), Pochonia chlamydosporia ( Yang et al, 2012 ), Clonostachys / Gliocadium ( Smith et al, 1990 ; Rodríguez et al, 2015 ) and P. oligandrum ( Martin and Hancock, 1986 ).…”