Isolation and Identification of Enteric Bacteria as Index Organisms for Milk Hygiene

Abstract

The present study was designed to isolate enteric bacteria from Egyptian raw milk. Twenty two samples were assembled randomly from local stores in Mansoura city and surrounding hamlets. Those samples are including Rayeb milk, buffalo farm milk, cow farm milk, bulk tank farm milk and bulk tank milk. A total of 136 of enteric bacterial isolates could be cultured from these samples by selective media and subjected to morphological and biochemical examinations. Out of these isolates, 2 cultures were isolated from cow farm milk and bulk tank farm milk and could be identified as Salmonella enterica and Staphylococcus aureus using 16S rRNA. Obtained results show that Egyptian raw milk can contain harmful bacteria such as Salmonella sp, E. coli and Staphylococcus sp, responsibility for causing many foodborne diseases.