Isolation and Identification of Endophytic Bacteria with Plant Growth Promoting Activity and Biocontrol Potential from Wild Pistachio Trees

Etminani, Faegheh; Harighi, Behrouz

doi:10.5423/ppj.oa.07.2017.0158

Cited by 118 publications

(38 citation statements)

References 52 publications

(54 reference statements)

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“…The most common endophytic orders detected in this work were the Enterobacterales and Pseudomonadales followed by Xanthomonadales and Bacillales. These results are consistent with other studies concerning the isolation of endophytic bacterial communities from host plants by culture-based methods [ 53 , 54 ] and with the bacterial diversity pattern previously reported for rice microbiome in other studies [ 55 , 56 ].…”

Section: Discussionsupporting

confidence: 93%

Endophytes from African Rice (Oryza glaberrima L.) Efficiently Colonize Asian Rice (Oryza sativa L.) Stimulating the Activity of Its Antioxidant Enzymes and Increasing the Content of Nitrogen, Carbon, and Chlorophyll

et al. 2021

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Bacterial endophytes support the adaptation of host plants to harsh environments. In this study, culturable bacterial endophytes were isolated from the African rice Oryza glaberrima L., which is well-adapted to grow with poor external inputs in the tropical region of Mali. Among these, six N-fixer strains were used to inoculate O. glaberrima RAM133 and the Asian rice O. sativa L. cv. Baldo, selected for growth in temperate climates. The colonization efficiency and the N-fixing activity were evaluated and compared for the two rice varieties. Oryza sativa-inoculated plants showed a fairly good colonization efficiency and nitrogenase activity. The inoculation of Oryza sativa with the strains Klebsiella pasteurii BDA134-6 and Phytobacter diazotrophicus BDA59-3 led to the highest nitrogenase activity. In addition, the inoculation of ‘Baldo’ plants with the strain P. diazotrophicus BDA59-3 led to a significant increase in nitrogen, carbon and chlorophyll content. Finally, ‘Baldo’ plants inoculated with Kl. pasteurii BDA134-6 showed the induction of antioxidant enzymes activity and the maintenance of nitrogen-fixation under salt stress as compared to the unstressed controls. As these endophytes efficiently colonize high-yielding crop varieties grown in cold temperate climates, they become good candidates to promote their growth under unfavorable conditions.

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Section: Discussionsupporting

confidence: 93%

Endophytes from African Rice (Oryza glaberrima L.) Efficiently Colonize Asian Rice (Oryza sativa L.) Stimulating the Activity of Its Antioxidant Enzymes and Increasing the Content of Nitrogen, Carbon, and Chlorophyll

et al. 2021

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“…Among the different treatment groups, infected kernels of the resistant line MI82 showed a significant increase in the population of Sphingomonas both at day 3 and day 7 or the relative abundance of Stenotrophomonas at day 3 compared to mock or infected kernels of the susceptible line SC212, respectively ( Table S2 ). Both these bacterial endophytes have been reported to demonstrate antimicrobial properties, including antifungal activity in diverse plant species [ 27 , 28 ]. Stenotrophomonas maltophilia isolated from the medicinal plant Fagonia indica showed higher amounts of total flavonoids, antioxidant capacity, and antimicrobial activities against fungal and bacterial pathogens [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

Changes in Bacterial Endophyte Community Following Aspergillus flavus Infection in Resistant and Susceptible Maize Kernels

Majumdar

Kandel

Cary

et al. 2021

IJMS

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Aspergillus flavus (A. flavus)-mediated aflatoxin contamination in maize is a major global economic and health concern. As A. flavus is an opportunistic seed pathogen, the identification of factors contributing to kernel resistance will be of great importance in the development of novel mitigation strategies. Using V3–V4 bacterial rRNA sequencing and seeds of A. flavus-resistant maize breeding lines TZAR102 and MI82 and a susceptible line, SC212, we investigated kernel-specific changes in bacterial endophytes during infection. A total of 81 bacterial genera belonging to 10 phyla were detected. Bacteria belonging to the phylum Tenericutes comprised 86–99% of the detected phyla, followed by Proteobacteria (14%) and others (<5%) that changed with treatments and/or genotypes. Higher basal levels (without infection) of Streptomyces and Microbacterium in TZAR102 and increases in the abundance of Stenotrophomonas and Sphingomonas in MI82 following infection may suggest their role in resistance. Functional profiling of bacteria using 16S rRNA sequencing data revealed the presence of bacteria associated with the production of putative type II polyketides and sesquiterpenoids in the resistant vs. susceptible lines. Future characterization of endophytes predicted to possess antifungal/ anti-aflatoxigenic properties will aid in their development as effective biocontrol agents or microbiome markers for maize aflatoxin resistance.

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“…EnB studies in rice ( Fujita et al, 2017 ), wheat ( Emami et al, 2019 ), tomato ( Khan et al, 2014 ), potato ( Sessitsch et al, 2004 ), sugarcane ( Mendes et al, 2007 ), and many other crops, medicinal plants, halophytes, and woody trees were mainly focussed, but endophytes from tea plants were rarely considered. To maintain stable symbiosis, endophytes ameliorate the host plant to tolerate abiotic and biotic stresses through phosphate solubilization ( Liu et al, 2014 ; Oteino et al, 2015 ), nitrogen fixation ( Hurek and Reinhold-Hurek, 2003 ; Zhu et al, 2012 ), indole-3-acetic acid (IAA) ( Patten and Glick, 1996 ; Hassan, 2017 ), siderophore ( Zhang et al, 2011 ), and 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase ( Zhang et al, 2011 ; Khan et al, 2014 ) synthesis and augment the resistance of plants to insects, pests, and pathogens by producing bioactive metabolites ( Etminani and Harighi, 2018 ) and polymer hydrolyzing enzymes such as cellulase, amylase, chitinase, and protease ( El-Deeb et al, 2012 ) and promote plant growth and development. Bacterial endophytes that have potentially beneficial effects on plant growth and development has been found in many genera including Arthrobacter, Azoarcus, Azospirillum, Azotobacter, Bacillus, Burkholderia, Curtobacterium, Erwinia, Gluconobacter, Klebsiella, Serratia, Pantoea, Herbaspirillum, Rahnella, Pseudomonas , and Xanthomonas ( Zinniel et al, 2002 ; Berg et al, 2005 ; Compant et al, 2011 ).…”

Section: Introductionmentioning

confidence: 99%

Prospecting Endophytic Bacteria Endowed With Plant Growth Promoting Potential Isolated From Camellia sinensis

et al. 2021

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Endophytes are well-acknowledged inoculants to promote plant growth, and extensive research has been done in different plants. However, there is a lacuna about the endophytes associated with tea clones and their benefit to promote plant growth. The present study focuses on isolating and characterizing the beneficial endophytic bacteria (EnB) prevalent in commercially important tea clones cultivated in North Eastern India as plant growth promoters. Diversity of culturable EnB microbiome, in vitro traits for plant growth promotion (PGP), and applicability of potent isolates as bioinoculant for in vivo PGP abilities have been assessed in the present study. A total of 106 EnB identified as members of phyla Proteobacteria, Firmicutes, and Actinobacteria were related to 22 different genera and six major clusters. Regarding PGP traits, the percentage of isolates positive for the production of indole acetic acid, phosphate solubilization, nitrogen fixation siderophore, ammonia, and 1-aminocyclopropane-1-carboxylic acid deaminase production were 86.8, 28.3, 78.3, 30.2, 95.3, and 87.7, respectively. In total, 34.0, 52.8, and 17.0% of EnB showed notable production of hydrolytic enzymes like cellulase, protease, and amylase, respectively. Additionally, based on the bonitur score, the top two isolates K96 identified as Stenotrophomonas sp. and M45 identified as Pseudomonas sp. were evaluated for biofilm formation, motility, and in vivo plant growth promoting activity. Results suggested strong biofilm formation and motility in K96 and M45 which may attribute to the colonization of the strains in the plants. Further in vivo plant growth promotion experiment suggested sturdy efficacy of the K96 and M45 as plant growth promoters in nursery condition in commercial tea clones Tocklai vegetative (TV) TV22 and TV26. Thus, this study emphasizes the opportunity of commercialization of the selected isolates for sustainable development of tea and other crops.

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Isolation and Identification of Endophytic Bacteria with Plant Growth Promoting Activity and Biocontrol Potential from Wild Pistachio Trees

Cited by 118 publications

References 52 publications

Endophytes from African Rice (Oryza glaberrima L.) Efficiently Colonize Asian Rice (Oryza sativa L.) Stimulating the Activity of Its Antioxidant Enzymes and Increasing the Content of Nitrogen, Carbon, and Chlorophyll

Endophytes from African Rice (Oryza glaberrima L.) Efficiently Colonize Asian Rice (Oryza sativa L.) Stimulating the Activity of Its Antioxidant Enzymes and Increasing the Content of Nitrogen, Carbon, and Chlorophyll

Changes in Bacterial Endophyte Community Following Aspergillus flavus Infection in Resistant and Susceptible Maize Kernels

Prospecting Endophytic Bacteria Endowed With Plant Growth Promoting Potential Isolated From Camellia sinensis

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