Isolation and identification of cutaneous leishmaniasis species by PCR–RFLP in Ilam province, the west of Iran

Kermanjani, Ali; Akhlaghi, Lame; Oormazdi, Hormozd; Hadighi, Ramtin

doi:10.1007/s12639-016-0772-7

Cited by 18 publications

(13 citation statements)

References 15 publications

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“…Ilam province, sandwiched between Kermanshah and Khuzestan provinces and Iraq, is also endemic for L. major. Two studies in Ilam and Mehran counties showed that all CL cases (n = 61/61 and n = 92/92, respectively) were infected by L. major (56,57).…”

Section: Western Iranmentioning

confidence: 99%

The Geographical Distribution of Cutaneous Leishmaniasis Causative Agents in Iran and Its Neighboring Countries, A Review

Ghatee

Taylor

Karamian

2020

Front. Public Health

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Leishmania tropica and Leishmania major are both the main cause of anthroponotic (ACL) and zoonotic cutaneous leishmaniasis (ZCL), respectively, in the Old World. Leishmania infantum and Leishmania donovani, which are important causes of visceral leishmaniasis, have also occasionally been reported in CL patients. The present study investigates the current distribution of causative species of CL in Iran and neighboring countries in the Middle East. There has been expansion of L. tropica into new urban and rural foci in Iran, with well-documented cases of visceralization, a substantial increase of CL in Syria, and the emergence of new foci and outbreaks in Turkey and Iraq, especially due to L. major. Civil war in Syria and Iraq, population movement, poverty, and climatic change play important roles in the changing CL distribution in this region. Control programs should adopt a multidisciplinary approach based on active surveillance and case finding, especially in vulnerable refugee populations, determination of hazard maps for CL hot points using GIS and other advanced technology, the free distribution of drugs, rodent control, and greater community engagement in poor and marginalized populations. Comprehensive molecular studies that could show the species and strains of Leishmania in different areas of each country can give a better view from the distribution of CL in this region.

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Section: Western Iranmentioning

confidence: 99%

The Geographical Distribution of Cutaneous Leishmaniasis Causative Agents in Iran and Its Neighboring Countries, A Review

Ghatee

Taylor

Karamian

2020

Front. Public Health

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“…A long border with Iraq, where no controls exist over leishmaniasis due to war and political problems, has resulted in an increased number of CL cases. A close distance to endemic CL foci such as Bushehr, Ilam, and Isfahan provinces affects new foci in Khuzestan province (16)(17)(18)(19). Several studies have been carried out in Khuzestan to molecularly characterize Leishmania spp.…”

Section: Introductionmentioning

confidence: 99%

Molecular Identification of Leishmania Species in an Outbreak of Re-Emerged Cutaneous Leishmaniasis in Southwestern Iran During 2015 - 2016

Kargar

Hajjaran

Moazen

et al. 2021

Arch Clin Infect Dis

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Background: Dezful and its suburbs, as the second city in Khuzestan Province, southwest of Iran, has been an endemic area of cutaneous leishmaniasis (CL) with a low incidence rate since the last decades. However, the disease incidence has rapidly increased, and now is considered as a re-emerging parasitic disease in the area. Objectives: The aim of this study was to identify the most prevalent CL species in Dezful Region. Methods: A total of 196 microscopically confirmed slides from CL patients referred to Dezful Health Center were randomly collected in the period of 2015 - 2016. After DNA extraction from microscopically positive slides, polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was carried out on 61 eligible specimens using ribosomal internal transcribed spacer 1 (ITS1) gene. The HaeIII restriction enzyme was used for the identification of species. Results: Samples were randomly collected from 196 acute CL cases, including 110 (56.2%) males and 86 (43.8%) females. Most infections were seen in the age range of 2 - 7 years (65/196, 33.1%). Totally, 60.1% of the cases had only one lesion, and half of the lesions appeared on hands. Furthermore, 162 (82.7%) cases were referred to Dezful Health Center in the cold seasons (autumn and winter). Results of PCR-RFLP on 61 eligible isolates showed that 60 (98.4%) isolates were Leishmania major, and only 1 (1.6%) isolate was Leishmania tropica. Conclusions: Our findings indicated that L. major is the main agent of re-emerged CL in Dezful and its suburbs, and the disease is a zoonosis.

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“…Por outro lado, o uso de urina a 5% colhida de pacientes nefropatas com diabetes mellitus induziu a uma maior curva de crescimento. Possivelmente, a proteinúria que ocorre nesses pacientes pode ter servido como fonte de aminoácidos, principais moléculas utilizadas para obtenção de energia pelo protozoário (SHAMSUZZAMAN et al, 1999 major confirmados por PCR-RFLP (KERMANJANI et al, 2017).…”

Section: Discussionunclassified

Uso Da Eletroquimioterapia No Tratamento De Carcinoma De Células Escamosas Em Um Felino

Pinheiro¹,

Pereira²,

Israel³

et al. 2020

Investigação Científica E Técnica Em Medicina Veterinária 2

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Direitos para esta edição cedidos à Atena Editora pelos autores.Todo o conteúdo deste livro está licenciado sob uma Licença de Atribuição Creative Commons. Atribuição 4.0 Internacional (CC BY 4.0).O conteúdo dos artigos e seus dados em sua forma, correção e confiabilidade são de responsabilidade exclusiva dos autores, inclusive não representam necessariamente a posição oficial da Atena Editora. Permitido o download da obra e o compartilhamento desde que sejam atribuídos créditos aos autores, mas sem a possibilidade de alterá-la de nenhuma forma ou utilizá-la para fins comerciais.A Atena Editora não se responsabiliza por eventuais mudanças ocorridas nos endereços convencionais ou eletrônicos citados nesta obra.

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Isolation and identification of cutaneous leishmaniasis species by PCR–RFLP in Ilam province, the west of Iran

Cited by 18 publications

References 15 publications

The Geographical Distribution of Cutaneous Leishmaniasis Causative Agents in Iran and Its Neighboring Countries, A Review

The Geographical Distribution of Cutaneous Leishmaniasis Causative Agents in Iran and Its Neighboring Countries, A Review

Molecular Identification of Leishmania Species in an Outbreak of Re-Emerged Cutaneous Leishmaniasis in Southwestern Iran During 2015 - 2016

Uso Da Eletroquimioterapia No Tratamento De Carcinoma De Células Escamosas Em Um Felino

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