Isolation and identification of cultivated bacteria associated with soybeans and their biocontrol activity against Phytophthora sojae

Arfaoui, Arbia; Adam, Lorne R.; Bezzahou, Abdellah; Daayf, Fouad

doi:10.1007/s10526-018-9873-9

Cited by 22 publications

(15 citation statements)

References 40 publications

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“…With the development of intestinal microbiology, it has been found that enteric microorganisms can be modified by genetic engineering to enrich and express insecticidal genes in the insect gut, which provides a feasible method for the use of microbes to prevent and control M. alternatus [72,73]. The prevention and control of plant diseases and pests by pathogenic bacteria has become a popular research topic and new pathogenic bacteria have been isolated and found continuously, enriching the possibilities of biological control of plant diseases and pests [74][75][76][77]. The vector of pine wilt disease and its intestinal microorganisms have a very important influence on host selection and colonization.…”

Section: Discussionmentioning

confidence: 99%

Characterization of bacterial communities associated with the pine wilt disease insect vectors Monochamus alternatus Hope and host trees Pinus massoniana

Guo¹,

Lin²,

Chen³

et al. 2019

Preprint

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Background Pine wilt disease (PWD) is a destructive disease caused by the pinewood nematode Bursaphelenchus xylophilus . Monochamus alternatus Hope is the main vector of this disease. The symbiotic microorganisms can play an important role in the transmission cycle mechanism. However, the role of bacterial microorganisms in the transmission of pine wood nematode by M. alternatus is not clear currently. The main purpose of this study is to reveal the composition and diversity of microbial flora in the gut of M. alternatus , as well as healthy and infected Pinus massoniana and its peripheral environment to discover the important microbial flora contributing to the transmission cycle.Methods In this study, total DNA was extracted from 60 samples, including 20 samples of M. alternatus gut from different larval instars, healthy P. massoniana , nematode-infected P. massoniana and their peripheral environment (needles, bark, phloem, xylem, root, surface soil and rhizosphere soil), by triplicate. Samples were used for 16S rDNA Amplicon sequencing to determine the composition and diversity of microbial flora in each sample.Results Infection of pinewood nematode resulted in an increase of the microbial community in the nematode-infected P. massoniana and its peripheral environment when compared with healthy P. massoniana , the microbial community in different tissues changed. Among them, Gryllotalpicola and Cellulomonas showed to be endemic microorganisms in nematode-infected P. massoniana , which can be used as indicators to detect the disease. Serratia was shown as an opportunistic pathogen, and was found to be enriched in M. alternatus gut and was also detected in the host plant tissues.Conclusions This study clarified the change of microbial community in the transmission of pine wilt disease by M. alternatus . An important theoretical basis for the prevention of pine wilt disease was structured by our research.

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Section: Discussionmentioning

confidence: 99%

Characterization of bacterial communities associated with the pine wilt disease insect vectors Monochamus alternatus Hope and host trees Pinus massoniana

Guo¹,

Lin²,

Chen³

et al. 2019

Preprint

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“…The dishes of P. sojae , inoculated by the above method, without the inoculation of Bacillus, were used as the control. According to the published methods [2,17], inhibition rates (I) of mycelium growth was calculated as I = [(R1 − R2)/R1] × 100, where R1 is the control mycelium growth between two discs edge. R2 is the mycelium growth toward the Bacillus .…”

Section: Methodsmentioning

confidence: 99%

“…Soybean endophytic bacteria, such as Enterobacter , Acinetobacter , Pseudomonas , Ochrobactrum , and Bacillus , associated with the nodules of soybean, can promote the growth of soybeans and inhibit the pathogenicity of P. sojae [16]. Arfaoui et al [17] found that Paenibacillus sp. and Streptomyces sp.…”

Section: Introductionmentioning

confidence: 99%

Biocontrol and Action Mechanism of Bacillus amyloliquefaciens and Bacillus subtilis in Soybean Phytophthora Blight

Liu

et al. 2019

IJMS

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With the improper application of fungicides, Phytophthora sojae begins to develop resistance to fungicides, and biological control is one of the potential ways to control it. We screened two strains of Bacillus; Bacillus amyloliquefaciens JDF3 and Bacillus subtilis RSS-1, which had an efficient inhibitory effect on P. sojae. They could inhibit mycelial growth, the germination of the cysts, and the swimming of the motile zoospores. To elucidate the response of P. sojae under the stress of B. amyloliquefaciens and B. subtilis, and the molecular mechanism of biological control, comparative transcriptome analysis was applied. Transcriptome analysis revealed that the expression gene of P. sojae showed significant changes, and a total of 1616 differentially expressed genes (DEGs) were detected. They participated in two major types of regulation, namely “specificity” regulation and “common” regulation. They might inhibit the growth of P. sojae mainly by inhibiting the activity of ribosome. A pot experiment indicated that B. amyloliquefaciens and B. subtilis enhanced the resistance of soybean to P. sojae, and their control effects of them were 70.7% and 65.5%, respectively. In addition, B. amyloliquefaciens fermentation broth could induce an active oxygen burst, NO production, callose deposition, and lignification. B. subtilis could also stimulate the systemic to develop the resistance of soybean by lignification, and phytoalexin.

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“…Root rot is caused by various pathogens and it is accelerated by high temperature, humidity, and high planting density [7]. Mycelia or spores present in soil or diseased plant residues enter host plant wounds and initiate infection.…”

Section: Introductionmentioning

confidence: 99%

Pathogenicity of fungus strains isolated from medicinal Fritillaria przewalskii Maxim. bulb rot

Chen

Guo

et al. 2020

Preprint

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Background: Bulb rot causes loss of the perennial medicinal plant Fritillaria przewalskii Maxim., is exacerbated with growth years and seriously constrains plant productivity, but the pathogens responsible for the disease were still unknown, very few reports were presented before.Methods: To determine potential pathogenic sources, fungus strains were isolated from diseased plant samples with bulb rot symptom of the 3- to 5-year-old F. przewalskii plants by tissue separation, and assayed for pathogenicity according to Koch’s Law.Results: Seven pathogenic strains (F1-F7) were detected in5-year-old rot bulbs, six (F1-F6) in 4-year-old rot bulbs, and four (F1, F2, F5, F6) in 3-year-old rot bulbs. All of the strains were able to infect bulbs by stabbing and some exhibited varying levels of aggressiveness. Relative to the non-stabbing controls, the bulbs stab-inoculated with F5, F2, F7, F4, F1, F6, and F3 showed 76.65%, 75.15%, 71.44%, 40.37%, 39.09%, 36.87%, and 34.93% rot after 8 days, respectively. Phylogenetic analysis revealed that theses trains (deposited in GenBank under accessions MH917682 to MH917688) were clustered into Bionectria ochroleuca (F1, F3, F4), Fusarium oxysporum (F2, F7), Fusarium tricinctum (F5), and Clonostachys rosea (F6). The two species of Fusarium had the strongest pathogenicity, followed by Bionectria ochroleuca and Clonostachys rosea. Although leading to low bulb rot incidence by stab-inoculation, F1 showed the highest isolation rate (48.9%) among all strains. Conclusions: Thus, the edible and medicinal bulbs of F. przewalskii are susceptible to synergetic contamination by these seven pathogenic strains at some point after their third year of growth, which has contributed to the species endangered status, with the two strains of Fusarium being the predominant pathogens. To our knowledge, this is the first report on the seven strains of four fungal species causing in F. przewalskii bulb rot in China.

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Isolation and identification of cultivated bacteria associated with soybeans and their biocontrol activity against Phytophthora sojae

Cited by 22 publications

References 40 publications

Characterization of bacterial communities associated with the pine wilt disease insect vectors Monochamus alternatus Hope and host trees Pinus massoniana

Characterization of bacterial communities associated with the pine wilt disease insect vectors Monochamus alternatus Hope and host trees Pinus massoniana

Biocontrol and Action Mechanism of Bacillus amyloliquefaciens and Bacillus subtilis in Soybean Phytophthora Blight

Pathogenicity of fungus strains isolated from medicinal Fritillaria przewalskii Maxim. bulb rot

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