Isolation and identification of cultivable lactic acid bacteria in traditional yak milk products of Gansu Province in China

Bao, Qiuhua; Liu, Wenjun; Yu, Jie; Wang, Weihong; Qing, Manjun; Xia, Chen; Wang, Fang; Zhang, JiaChao; Zhang, Wenyi; Qiao, Jun; Sun, Tiansong; Zhang, Heping

doi:10.2323/jgam.58.95

Cited by 44 publications

(34 citation statements)

References 27 publications

(37 reference statements)

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“…Samples (1 ml) were mixed in 9 ml of 0.85% (w/v) sterile physiological saline. Serial dilution (10 -1 -10 -8 ) was prepared from the supernatant and the dilutions (10 -5 -10 -8 ) were plated in duplicate on an M17 medium (Oxoid Ltd., Basingstoke, UK) containing 0.01% (v/v) cycloheximide and polymyxin (Bao et al, 2012). Plates were incubated under anaerobic conditions at 30°C, 37°C and 42°C for 48 h. Finally, 30°C was chosen as the best growth temperature for cocci since lower survival rates were observed with the two other considered temperatures.…”

Section: Methodsmentioning

confidence: 99%

“…However, S. thermophilus dominated, contributing to 33% of all cocci isolates. Bao et al (2012) also reported that S. thermophilus was the dominating species in Qula cheese (39%), whereas Tan et al (2010) and Duan et al (2008) reported that the dominating LAB was the Leuconostoc species (86% and 78%, respectively).…”

Section: Enumeration Isolation and Identification Of Lab From Qula Cmentioning

confidence: 99%

“…In particular, LAB plays an essential role in the formation of the aroma, texture, and acidity of the products. Previous studies showed that LAB is the dominant species in the microorganisms of Qula cheese, and play the most important role in the accumulation of nutrients (Bao et al, 2012;Duan et al, 2008). Some of the lactic acid bacteria are also known to have beneficial effects on human health (Mohd Adnan and Tan, 2007;Zhang, W. Y. et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Study on <i>Streptococcus thermophilus</i> isolated from Qula and associated characteristic of acetaldehyde and diacetyl in their fermented milk

Zha

Zhang

et al. 2015

J. Gen. Appl. Microbiol.

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Section: Methodsmentioning

confidence: 99%

Section: Enumeration Isolation and Identification Of Lab From Qula Cmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Study on <i>Streptococcus thermophilus</i> isolated from Qula and associated characteristic of acetaldehyde and diacetyl in their fermented milk

Zha

Zhang

et al. 2015

J. Gen. Appl. Microbiol.

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“…Leuconostoc lactis isolate MAU80137 was the only isolate from pickle (Sichuan province). All isolates were identified as L. lactis based on standard physiological and biochemical tests, and sequence analysis of the 16S rRNA gene [32,49]. Stock cultures were stored in 10% glycerol at -80°C.…”

Section: Methodsmentioning

confidence: 99%

A novel multi-locus sequence typing (MLST) protocol for Leuconostoc lactis isolates from traditional dairy products in China and Mongolia

et al. 2014

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BackgroundEconomically, Leuconostoc lactis is one of the most important species in the genus Leuconostoc. It plays an important role in the food industry including the production of dextrans and bacteriocins. Currently, traditional molecular typing approaches for characterisation of this species at the isolate level are either unavailable or are not sufficiently reliable for practical use. Multilocus sequence typing (MLST) is a robust and reliable method for characterising bacterial and fungal species at the molecular level. In this study, a novel MLST protocol was developed for 50 L. lactis isolates from Mongolia and China.ResultsSequences from eight targeted genes (groEL, carB, recA, pheS, murC, pyrG, rpoB and uvrC) were obtained. Sequence analysis indicated 20 different sequence types (STs), with 13 of them being represented by a single isolate. Phylogenetic analysis based on the sequences of eight MLST loci indicated that the isolates belonged to two major groups, A (34 isolates) and B (16 isolates). Linkage disequilibrium analyses indicated that recombination occurred at a low frequency in L. lactis, indicating a clonal population structure. Split-decomposition analysis indicated that intraspecies recombination played a role in generating genotypic diversity amongst isolates.ConclusionsOur results indicated that MLST is a valuable tool for typing L. lactis isolates that can be used for further monitoring of evolutionary changes and population genetics.

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“…These products are usually fermented by natural microorganism. Thus, the dairy products microflora is largely determined by the local environmental conditions (Bao et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Isolation and identification of yeast in yak milk dreg of Tibet in China

Jun-jun

Guo

et al. 2014

Dairy Sci. & Technol.

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International audienceYak milk dreg is a popular dairy product with a unique flavor which is frequently consumed in Tibet. The aim of this study was to investigate the occurrence and distribution of yeasts in yak milk dreg of Tibet. Ninety-one yeast colonies were isolated from ten yak milk dreg samples, and the isolates belonged to seven genera and ten different species based on the phenotypic characterization and analysis of ITS/5.8S ribosomal DNA (rDNA) gene. The distribution of each species among ten samples was as follows: Kluyveromyces lactis (6/10), Pichia fermentans (6/10), Saccharomyces cerevisiae (6/10), Rhodotorula mucilaginosa (6/10), Kluyveromyces marxianus (5/10), Pichia kudriavzevii (4/10), Torulaspora delbrueckii (2/10), Torulaspora quercuum (2/10), Debaryomyces hansenii (1/10), and Cryptococcus albidus (1/10). The present study reports for the first time the yeast profile in yak milk dreg products of Tibet in China, which provides useful information and yeast strain resource for further studies involving probiotic strain selection and starter culture design for the industrial production of yak milk dreg

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Isolation and identification of cultivable lactic acid bacteria in traditional yak milk products of Gansu Province in China

Cited by 44 publications

References 27 publications

Study on <i>Streptococcus thermophilus</i> isolated from Qula and associated characteristic of acetaldehyde and diacetyl in their fermented milk

Study on <i>Streptococcus thermophilus</i> isolated from Qula and associated characteristic of acetaldehyde and diacetyl in their fermented milk

A novel multi-locus sequence typing (MLST) protocol for Leuconostoc lactis isolates from traditional dairy products in China and Mongolia

Isolation and identification of yeast in yak milk dreg of Tibet in China

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