2019
DOI: 10.21608/bvmj.2019.17855.1106
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Isolation and identification of Cronobacter species from some animal sources

Abstract: This work was carried out to study the bacteriological importance of Cronobacter sakazakiias a potential foodborne emerging pathogen involved in severe illness and deaths in humans, especially neonates due to consumption of contaminated infant powdered infant formula (PIF) (PIF milk and food). A total of 100 samples [PIF milk (n=55), PIF food (n=15), milk powder (n=15) and milk powder products (n=15) were collected and subjected to bacteriological examination for the presence of Cronobacter. Six samples out of… Show more

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“…Five selected C. sakazakii isolates (one isolate from the examined samples of powdered milk infant formula, milk powder, minced meat, cow milk, and diarrheic infant stool) were tested for detection of Cronobacter 16S rRNA gene by using QIAamp® DNA Mini Kit instructions (Qiagen, Germany, GmbH), Emerald Amp GT PCR mastermix (Takara, Japan). PCR technique was done by the primer as shown in table (1) and running conditions: one cycle of denaturation at 94℃ for 5 min, followed by 35 cycles of 94℃ for 30 s, 52℃ for 40 s, and 72℃ for 1min. PCR was completed with a final extension at 72C for 10 min.…”
Section: Genotypic Identification Of Some C Sakazakii Isolates By Pcrmentioning
confidence: 99%
“…Five selected C. sakazakii isolates (one isolate from the examined samples of powdered milk infant formula, milk powder, minced meat, cow milk, and diarrheic infant stool) were tested for detection of Cronobacter 16S rRNA gene by using QIAamp® DNA Mini Kit instructions (Qiagen, Germany, GmbH), Emerald Amp GT PCR mastermix (Takara, Japan). PCR technique was done by the primer as shown in table (1) and running conditions: one cycle of denaturation at 94℃ for 5 min, followed by 35 cycles of 94℃ for 30 s, 52℃ for 40 s, and 72℃ for 1min. PCR was completed with a final extension at 72C for 10 min.…”
Section: Genotypic Identification Of Some C Sakazakii Isolates By Pcrmentioning
confidence: 99%