Isolation and identification of Clostridium perfringens from milk samples and dairy products of Quetta City, Pakistan

Abstract

The concern study was conducted to isolate the causal agent of food poisoning in milk samples and dairy products from various zones of Quetta city. Total 1120 samples were collected, out of that 19.28 % were positive for Clostridium perfringens while 80.71% were found negative. The zone wise distribution of positive cases shows 3.57% from east zone, 2.8% from west zone, 5.71% from north zone, and 7.14% from south zone of Quetta city. The milk and dairy products of south zone were highly infected with Clostridium perfringens as compared to all three zones of Quetta city. The confirmation of Clostridium perfringens was done through gram staining, various biochemical tests and PCR. The PCR result displayed clear band of 541 base pairs CPE gene under UV light. Different drugs result showed that C. perfringens was sensitive to Amoxicillin (23mm), Penicillin G (16mm), Vancomycin (24mm), Gentamycin (20mm), Streptomycin (15mm), Chloramphenicol (26mm), Ciprofloxacin (24mm), Kanamycin (18mm), Amikacin (14mm), while high resistant was shown against