Isolation and Identification of cellulolytic bacteria from mangrove sediment in Bangka Island

Kurniawan, Andi; Prihanto, Asep Awaludin; Sari, Suci Puspita; Febriyanti, Dwi Yandhi; Sambah, Abu Bakar; Asriani, Euis

doi:10.1088/1755-1315/137/1/012070

Cited by 18 publications

(18 citation statements)

References 8 publications

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“…Several studies over many years were interested in the isolation and characterization of cellulose degrading bacteria more than fungi, because bacteria have elevated growth rate and more resistance to an extreme environment, as compared with fungi (Lynd et al, 2002;Odeniyi et al, 2009;Rasul et al, 2015). Bacteria belonging to several genera including; Cellulomonas, Cellulovibrio, Pseudomonas, Bacillus, Bacteroides, Acetovibrio, Streptomyces and Micrococcus, produce different types of cellulase (Immanuel et al, 2006;Kurniawan et al, 2018). Patil et al, (2000) demonstrated that chitin is one of the most common biodegradable polymer after cellulose, which exists naturally in the biosphere as a structural polysaccharide of β-1,4-N-acetyl-Dglucosamin.…”

Section: Introductionmentioning

confidence: 99%

Optimization of cellulase and chitinase enzymes production by plant growth promoting rhizobacteria

2020

Novel Research in Microbiology Journal

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The current study aimed to optimize the production of cellulase and chitinase enzymes by plant growth promoting rhizobacteria (PGPR). Bacteria were pre-isolated from soil and identified as; Pseudomonas fluorescens NBRC (KW1), Serratia liquefaciens ATCC 27592 (EW1), Bacillus subtilis SBMP4 (EF1) and Bacillus megaterium NBRC 15308 (TF2). The effect of different growth parameters including; pH, temperature, carbon and nitrogen sources, and incubation periods were optimized for production of cellulase and chitinase enzymes by the selected bacterial strains. Three strains mainly, B. subtilis SBMP4, S. liquefaciens ATCC 27592 and P. fluorescens NBRC) recorded positive results for cellulase production. B. subtilis SBMP4 (EF1) and S. liquefaciens ATCC 27592 (EW1) strains demonstrated significant ability to produce cellulase at 40°C, while P. fluorescens NBRC (KW1) strain showed the maximum enzyme production at 30°C. Carboxymethyl cellulose gave the highest cellulase production compared to the other carbon sources. Chitinase enzyme was optimally produced by B. subtilis SBMP4 and S. liquefaciens ATCC 27592 strains under primary screening. B. subtilis SBMP4 had the strongest ability to produce chitinase at 40 o C, while S. liquefaciens ATCC 27592 at 30 o C. The optimum pH observed was at pH 6.0 to get the maximum chitinase production by S. liquefaciens ATCC 27592. Potassium nitrate (KNO 3 ) as an inorganic nitrogen source presented the highest chitinase production by B. subtilis SBMP4, whereas yeast extract demonstrated significant chitinase production by S. liquefaciens ATCC 27592. Mashhoor, W.A.; Nasr, S.A.; Sharaf, M.S. and Abdel-Azeem, H.H. (2009). Nutritional and environmental factors affecting cellulase production by two strains of cellulolytic Bacilli.

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Section: Introductionmentioning

confidence: 99%

Optimization of cellulase and chitinase enzymes production by plant growth promoting rhizobacteria

2020

Novel Research in Microbiology Journal

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“…Based on phylogenetic trees, pseudomonas psychotolerans has a close relationship with P. oleovorans and P. putida. Psedomonas genus was also identified in cellulolytic bacteria from mangrove sediments in Tukak Sadai, southern Bangka [22], mangrove Kraton Pasuruan [13], mangrove soil in the Gunung Anyar river estuary, Surabaya [23], mangrove sediments in the Hong Kong nature reserve [24], Bajul Mati Beach, Malang, East Java [25] and natural mangrove of Great Nicobar Island, India [26].…”

Section: Resultsmentioning

confidence: 99%

Culturable Cellulolytic Bacteria From Mangrove With Anadara Granosa Cultivation In Sukal, West Bangka

Kurniawan¹,

Sari²,

Asriani³

et al. 2019

Proceedings of the International Conference on Maritime and Archipelago (ICoMA 2018)

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Sukal, West Bangka district became one of the centers of Anadara sp culture. Not yet known the effect of shellfish cultivation on the ability of decomposition in mangroves. Cellulolytic bacteria are part of degradation in mangroves. Cellulolytic bacteria have cellulose degradation ability and it made carbohydrates are easier about digested for livestock. This research aims to obtained cellulolytic bacteria in soil, decayed wood and leaf from mangrove with Anadara sp culture in Sukal, West Bangka. The research was conducted from March until June 2018. Isolation was done using agar media with 1% Carboxymethyl Cellulose (CMC). An isolate of bacteria grew in agar with 1% CMC and given the Lugol and Congo Red at 72 hours to test cellulose degradation ability. Isolates from Sukal Mangrove that result the largest cellulose degradation had their species identification using 16S rRNA gene sequenced and microbact analysis. The results of biochemical tests using Mikrobact for bacterial isolates cellulolytic show selected lead to species Bacillus megaterium, Citrobacter freundii, and Vibrio alginoliticus. DNA sequences of isolate bacteria showed close kinship with Pseudomonas psychotolerans.

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“…Resistensi bakteri dalam kondisi kontaminasi memungkinkan adaptasi dan pemilihan bakteri sehingga bakteri diidentifikasi berbeda dengan mangrove di daerah lain. Kurniawan et al (2018b) Isolat bakteri dengan kemampuan degradasi selulosa terbesar di kultur pada media nutrient broth dan diinkubasi selama 3 hari untuk selanjutnya diidentifikasi dengan metode 16S rRNA. Isolasi DNA dilakukan dengan menggunakan prosedur Kit Isolasi DNA (Wizard of Genomic DNA Purification Kit dari Promega).…”

Section: Pendahuluanunclassified

Identifikasi Molekuler Isolat Bakteri Selulolitik Dari Mangrove Sungailiat Dan Tukak Sadai Di Pulau Bangka

Kurniawan

Sari

Asriani

et al. 2018

j.eng

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Bakteri selulolitik memiliki kemampuan degradasi selulosa dan membuat karbohidrat lebih mudah dicerna bagi ternak. Penelitian ini bertujuan untuk mengidentifikasi bakteri selulolitik di tanah, kayu lapuk dan daun dari mangrove pulau Bangka melalui skrining dan analisis gen 16s rRNA. Penelitian dilakukan dari September 2017 hingga Maret 2018. Isolat TSL7 dan TSS4 dari Mangrove Tukak Sadai dan SLS5 dari Mangrove Sungailiat yang memiliki kemampuan mendegradasi selulosa terbesar berdasarkan hasil skrining menjadi isolat yang diidentifikasi pada gen 16S rRNA untuk diurutkan dan analisis BLAST. Analisis gen bank menunjukkan kedekatan dengan Pseudomonas aeruginosa dengan query cover 37% - 87%. Hasil query cover rendah dihasilkan karena sekuens yang sesuai pendek. Kontaminasi dan perubahan komunitas karena seleksi lingkungan dengan adanya logam berat dapat menjadi penyebab dominasi Pseudomonas aeruginosa. Pseudomonas aeruginosa yang bersifat patogen tidak disarankan untuk diaplikasikan sebagai pendegradasi selulosa pakan ternak.

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Isolation and Identification of cellulolytic bacteria from mangrove sediment in Bangka Island

Cited by 18 publications

References 8 publications

Optimization of cellulase and chitinase enzymes production by plant growth promoting rhizobacteria

Optimization of cellulase and chitinase enzymes production by plant growth promoting rhizobacteria

Culturable Cellulolytic Bacteria From Mangrove With Anadara Granosa Cultivation In Sukal, West Bangka

Identifikasi Molekuler Isolat Bakteri Selulolitik Dari Mangrove Sungailiat Dan Tukak Sadai Di Pulau Bangka

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