Isolation and identification of cancer stem cells from a side population of a human hepatoblastoma cell line, HuH-6 clone-5

Hayashi, S.; Fujita, Keiko; Matsumoto, Sachiko; Akita, Masumi; Satomi, Akira

doi:10.1007/s00383-010-2719-x

Cited by 18 publications

(17 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…27,28 In HB, a side population (SP) was successfully isolated from a HB cell line which was able to form tumors in mice whereas tumors did not develop in the non-SP inoculated mice. 13,29 However, a single study has been done on human clinical samples which included HCC and 4 HB cases, of which one case only was positive for CSC markers. 14 In the current study the CSC markers CD44, CD90 and CD133 proteins; were respectively expressed in 48.8%, 32.6% and 58.1% of the cases, compared to 46.5%, 32.6% and 41.7% for RNA.…”

Section: Discussionmentioning

confidence: 99%

“…A case was considered positive, for CD133, CD44 or CD90, if brown membranous and/or cytoplasmic immunostaining was detected in >10% of the cells. [13][14] …”

Section: Immunohistochemistry (Ihc)mentioning

confidence: 99%

“…Some markers as CD133 and CD44 were used to detect HB-CSCs in cultured human HB cells, while CD90 was reported to be a CSC marker in hepatocellular carcinoma. 13 …”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Hepatoblastoma Survival and the Prognostic Role of Cancer Stem Cell Markers

Fawzy¹,

Bahnassy

El-Wakil

et al. 2014

Int J Cancer Ther Oncol

View full text Add to dashboard Cite

Original ArticleAbstract Purpose: Hepatoblastoma (HB) is an embryonal tumor of the liver that occurs in infants and young children. Complete surgical resection and cisplatin-containing chemotherapy are crucial for cure in HB. Cancer stem cells (CSCs) constitute a newly identified subpopulation, which may differentiate into heterogeneous progenies of malignant cells. The aim of this study was to assess the survival outcome and the prognostic value of CSCs markers (CD133, CD90 and CD44) in a cohort of HB patients from Egypt. Methods: Disease status of 43 HB patients was evaluated at the main checkpoints of therapy and during follow-up. Treatment included surgical tumor resection and systemic chemotherapy (cisplatin, 5-fluorouracil, doxorubicin, and vincristine). Protein and RNA expressions of CD44, CD 90 and CD 133 were assessed by immunohistochemistry and quantitative PCR. Results:The OS for all patients was 58.2 at 4 years. Patients with localized disease stages (I&II) had a better OS than those with advanced stages (III&IV) (81.9% versus 30%, p<0.001). Total surgical resection was superior to incomplete/no resection (83.8% versus 25.2 %; p<0.001). The OS was significantly correlated with tumor response (p<0.001) and each of CD44, CD 90, CD 133 expression (p<0.001) whereas reduced DFS was associated with CD44 and CD133 expression (p<0.001). Conclusion: Localized disease is associated with higher OS than more advanced stages III and IV. Complete surgical resection facilitated with systemic preoperative chemotherapy in initially irresectable cases can improve survival in HB while CSC markers (CD133, 44, and 90) can predict survival and response to treatment in HB patients.

show abstract

Section: Discussionmentioning

confidence: 99%

“…A case was considered positive, for CD133, CD44 or CD90, if brown membranous and/or cytoplasmic immunostaining was detected in >10% of the cells. [13][14] …”

Section: Immunohistochemistry (Ihc)mentioning

confidence: 99%

See 1 more Smart Citation

Hepatoblastoma Survival and the Prognostic Role of Cancer Stem Cell Markers

Fawzy¹,

Bahnassy

El-Wakil

et al. 2014

Int J Cancer Ther Oncol

View full text Add to dashboard Cite

show abstract

“…When the irradiation dose increased to 30 Gy, the number of adherent cells further decreased until all cells were dead, whereas after irradiation of suspended cells at 45 Gy, some cells were still alive (Figure 6). (Richardson et al, 2004;Collins et al, 2005), retinoblastoma (Seigel et al, 2005), pancreatic cancer , and hepatoblastoma (Hayashi et al, 2011). Animal models of human tumors have been shown to contain gastric cancer stem cells (Houghton et al, 2004) and lung cancer stem cells (Noh et al, 2009).…”

Section: Changes In Cell Growth and Expression Of Cd44 After Exposurementioning

confidence: 99%

Growth, Clonability, and Radiation Resistance of Esophageal Carcinoma-derived Stem-like Cells

Liu²,

Yang³

et al. 2013

Asian Pacific Journal of Cancer Prevention

View full text Add to dashboard Cite

Objective: To separate/enrich tumor stem-like cells from the human esophageal carcinoma cell line OE-19 by using serum-free suspension culture and to identify their biological characteristics and radiation resistance. Methods: OE-19 cells were cultivated using adherent and suspension culture methods. The tumor stem-like phenotype of CD44 expression was detected using flow cytometry. We examined growth characteristics, cloning capacity in soft agar, and radiation resistance of 2 groups of cells. Results: Suspended cells in serum-free medium formed spheres that were enriched for CD44 expression. CD44 was expressed in 62.5% of suspended cells, but only in 11.7% of adherent cells. The suspended cells had greater capacity for proliferation and colony formation in soft agar than the adherent cells. When the suspended and adherent cells were irradiated at 5 Gy, 10 Gy, or 15 Gy, the proportion of CD44+ suspended cells strongly and weakly positive for CD44 was 77.8%, 66.5%, 57.5%; and 21.7%, 31.6%, 41.4%, respectively. In contrast, the proportion of CD44+ adherent cells strongly positive for CD44 was 18.9%, 14.%, and 9.95%, respectively. When the irradiation dose was increased to 30 Gy, the survival of the suspended and adherent cells was significantly reduced, and viable CD44+ cells were not detected. Conclusion: Suspended cell spheres generated from OE-19 esophageal carcinoma cells in serum-free stem medium are enriched in tumor stem-like cells. CD44 may be a marker for these cells.

show abstract

“…All but one cell line (Ewing’s sarcoma) contained a SP ranging from 0.12% to 14.6%. Similarly, in separate studies, several osteosarcoma cell lines were found to have SP cells that were capable of forming spherical colonies and inducing tumorigenesis, and a SP from a hepatoblastoma cell line was able to form tumors in mice whereas tumors did not form in the non-SP [75,76]. In each of these studies, the resistance patterns of the SP were not tested.…”

Section: Tumor Markersmentioning

confidence: 99%

Cancer Stem Cells and Pediatric Solid Tumors

Friedman

Gillespie

2011

Cancers

View full text Add to dashboard Cite

Recently, a subpopulation of cells, termed tumor-initiating cells or tumor stem cells (TSC), has been identified in many different types of solid tumors. These TSC, which are typically more resistant to chemotherapy and radiation compared to other tumor cells, have properties similar to normal stem cells including multipotency and the ability to self-renew, proliferate, and maintain the neoplastic clone. Much of the research on TSC has focused on adult cancers. With considerable differences in tumor biology between adult and pediatric cancers, there may be significant differences in the presence, function and behavior of TSC in pediatric malignancies. We discuss what is currently known about pediatric solid TSC with specific focus on TSC markers, tumor microenvironment, signaling pathways, therapeutic resistance and potential future therapies to target pediatric TSC.

show abstract

Isolation and identification of cancer stem cells from a side population of a human hepatoblastoma cell line, HuH-6 clone-5

Abstract: Cancer stem-like cells were separated by the SP fraction method from hepatoblastoma cells. The in vivo experiment proved that SP fraction cells inoculated into mice were self-replicated, and the existence of cancer stem-like cells was identified.

Cited by 18 publications

References 26 publications

Hepatoblastoma Survival and the Prognostic Role of Cancer Stem Cell Markers

Hepatoblastoma Survival and the Prognostic Role of Cancer Stem Cell Markers

Growth, Clonability, and Radiation Resistance of Esophageal Carcinoma-derived Stem-like Cells

Cancer Stem Cells and Pediatric Solid Tumors

Contact Info

Product

Resources

About