Isolation and identification of bacterial pathogen from mastitis milk in Central Java Indonesia

Harjanti, Dian Wahyu; Ciptaningtyas, Rizke; Wahyono, Fajar; Setiatin, ET

doi:10.1088/1755-1315/102/1/012076

Cited by 13 publications

(12 citation statements)

References 14 publications

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“…While Staphylococci consist of forty-five species and twenty-one subspecies that are known to be the key causes of mastitis in dairy animals, there is still debate over the clinical relevance of S. aureus isolated from animals infected with mastitis. Some regard S. aureus as a main carrier of mastitis, while others view it as a minor carrier of the disease (Harjanti et al, 2018). Thus, the present study examined seemingly healthy animals and others that presented symptoms of mastitis; 54 S. aureus isolates and 66 CNS isolates were correctly recognized by using tube coagulase test and later by the Vitek™ 2 compact system.…”

Section: Discussionmentioning

confidence: 91%

Comparison of MALDI-TOF mass spectrometry with phenotypic methods for identification and characterization of Staphylococcus aureus causing mastitis

et al. 2021

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The emergence of methicillin-resistant Staphylococcus aureus (MRSA) infection is one of the greatest threats to both animal and human health. Our investigation was aimed to identify and differentiate between MRSA and methicillin-sensitive Staphylococcus aureus (MSSA) recovered from mastitic milk using MALDI-TOF mass spectrometry compared with phenotypic methods and studying their susceptibility to various antibiotics. Four hundred milk samples from mastitic animals (cows, sheep, goats, and dromedary camels) were investigated. Phenotypic identification of S. aureus was made through MASTASAPH Latex test, STAPH ID 32, and Vitek 2 system. The proteomic characterization of S. aureus was done by MBT. The Kirby Bauer method was accomplished to detect the resistance of S. aureus strains to antibiotics. The results of the MASTASAPH Latex test, revealed that 54 (46%) were recognized as S. aureus. All S. aureus isolates were identified by MBT with a score of more or equal 2.00. Several peaks were identified in the mass of 4590 Da, 4863 Da, and 4938 Da for MSSA and in the mass of 2636 Da and 3009 Da for MRSA. The MSP dendrogram demonstrated that the S. aureus isolates were classified into one group with a distance level of less or equal 400. The percentage of S. aureus resistance against carbenicillin, erythromycin and kanamycin was 94.4%, 38.88%, and 33.33%, respectively. In conclusion, S. aureus bacteria are among the key triggers for mastitis in Saudi Arabia. MBT is reported to be not only the rapid tool to identify S. aureus but also able to discriminate MRSA from MSSA.

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Section: Discussionmentioning

confidence: 91%

Comparison of MALDI-TOF mass spectrometry with phenotypic methods for identification and characterization of Staphylococcus aureus causing mastitis

et al. 2021

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“…To date, several studies have explored the use of herbal remedies against bacterial isolates from mastitis-infected animals [ 6 , 7 , 16 , 21 ]. Reportedly, S. aureus and E. coli are the leading pathogens accountable for contagious mastitis in ruminants [ 16 , 17 , 31 ]. The effect of mastitis primarily depends on the etiology.…”

Section: Discussionmentioning

confidence: 99%

“…The isolation procedure was performed as described by Harjanti et al . [ 17 ]. The bacteria were subcultured on house prepared 5% sheep blood agar (Oxoid™ Blood Agar Base CM0055).…”

Section: Methodsmentioning

confidence: 99%

“…Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria were isolated from mastitis-infected cows, which were reared in the local dairy farms in Central Java Province. The isolation procedure was performed as described by Harjanti et al [17]. The bacteria were subcultured on house prepared 5% sheep blood agar (Oxoid Blood Agar Base CM0055).…”

Section: Mastitis Bacteria Preparationmentioning

confidence: 99%

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Effects of different sterilization methods of herbal formula on phytochemical compounds and antibacterial activity against mastitis-causing bacteria

2020

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Background and Aim: The current phytobiotic industry is struggling to determine a proper sterilization method for an herbal formula that comprises multiple plant extracts. Hence, this study aims to investigate the effects of two sterilization methods of herbal formula on phytochemical compounds and antibacterial activity against mastitis-causing bacterial isolates. Materials and Methods: The herbal formula comprised the extracts of Piper betle leaves, Curcuma domestica, and Curcuma zanthorriza. We applied two sterilization methods – sterilization using 0.45 μM syringe filter and sterilization using an autoclave at 121°C for 15 min. After sterilization, we measured phytochemical contents (phenol and flavonoid) of the herbal formula. Using the disk diffusion method, the antibacterial susceptibility test of the sterilized herbal formula against mastitis-causing bacterial isolates was conducted. Tetracycline, erythromycin, and penicillin – common antibiotics for mastitis therapy in dairy farms – were used as standard antibiotics. Results: Compared with autoclave sterilization, syringe filter sterilization resulted in less (p<0.05) phenolic and flavonoid contents. Against Escherichia coli and Staphylococcus aureus, the filter sterilized herbal formula (E. coli: 65.9%-73%; S. aureus: 6.2%-18.1%) markedly reduced the antibacterial activity than the autoclave-sterilized herbal formula (E. coli: 2.1%- 3%; S. aureus: 4.5%-10.7%). Conclusion: This study establishes that autoclave sterilization of the herbal formula is the best sterilization method that exerts minimal adverse effects on the phytochemical compounds and could sustain the antibacterial efficacy against mastitis-causing bacteria. Hence, the herbal formula could be used as an alternative treatment for bovine mastitis.

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“…Mastitis is a disease of economic importance in the dairy sector [1,2,3]. It is caused by coliform bacteria, staphylococci and streptococci with a range of clinical appearances such as fever, feed withdrawal and reduced milk production [4,5,6,7]. Dairy cows exposed to mastitis do express a complex interaction between the cow's defence mechanism and the specific bacterial virulence factors like; toxins, polysaccharide, cell surface factors, secreted factors, gene, biofilm, capsule and membrane proteins [8,9,10].…”

Section: Introductionmentioning

confidence: 99%

Acute Phase Proteins (Haptoglobin and Serum Amyloid A) and Pro-Inflammatory Cytokines (IL-12 and IL-10) in Cows Vaccinated with Prototype Killed S. aureus Mastitis Vaccine and Challenged with S. aureus Mastitis Infection.

Hambali

Abdallah

Bhuttu

et al. 2020

Preprint

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Background The economic downturn experienced by farmers and the fear of milk borne infection are of a greater public health concern. Haptoglobin, Serum Amyloid A, IL-12 and IL-10 in lactating Friesian cows vaccinated with prototype killed S. aureus mastitis vaccine and challenged with S. aureus were evaluated. Bacterin concentration at 10 8 cfu /ml of the local isolate of S. aureus was adjuvanted with KAl(SO₄)₂. Six lactating Friesian cows were grouped into A= Negative control, B = Positive control and C = vaccine group. Group C was vaccinated intramuscularly with 2ml of the monovalent vaccine, groups A and B with physiologic normal saline. Groups B and C were later challenged with the live bacterium via intramammary route . Result There was a significant increase in IL-10 concentration in vaccinated group post primary vaccination (PPV), booster phase (PB) and during the bacterial challenge phase. There was also a significantly increased IL-12 concentration in the vaccinated group at 24 hours, weeks 1 and 2 PPV. Haptoglobin at 12 and 24 hours PPV had a significant difference in group C. During the PB at 8 and 12 hours there was a significant difference in group C. During the bacterial challenged phase at 0, 3, 24 hours and day 7 PC there was a significant difference in group B. At 8 hours PC there was a significant difference in group C. For Serum Amyloid A, during PPV at 0, 3, 8, 12, 24 hours and weeks 1 and 2, the concentrations was significantly different in groups C. During PB at 0, 3, 8 and 12 hours PB there was a significant difference in groups C. During the bacterial challenge phase at 3, 8, 12, 24 hours, days 7 and 14 PC there was a significant difference in group B. At 0 hour PC there was a significant increase observed in group C. Conclusion The developed prototype killed S. aureus mastitis vaccine using local isolates was able to stimulate acute phase proteins and pro-inflammatory cytokines. The pattern of responses PC indicated protection, thereby suggesting that vaccination can protect against mastitis infection in dairy cows.

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Isolation and identification of bacterial pathogen from mastitis milk in Central Java Indonesia

Cited by 13 publications

References 14 publications

Comparison of MALDI-TOF mass spectrometry with phenotypic methods for identification and characterization of Staphylococcus aureus causing mastitis

Comparison of MALDI-TOF mass spectrometry with phenotypic methods for identification and characterization of Staphylococcus aureus causing mastitis

Effects of different sterilization methods of herbal formula on phytochemical compounds and antibacterial activity against mastitis-causing bacteria

Acute Phase Proteins (Haptoglobin and Serum Amyloid A) and Pro-Inflammatory Cytokines (IL-12 and IL-10) in Cows Vaccinated with Prototype Killed S. aureus Mastitis Vaccine and Challenged with S. aureus Mastitis Infection.

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