Isolation and Identification of Antimicrobial Proteins From the Leaves of Valeriana Hardwickii and Senna Obtusifolia

N, Madhuri Harsha; Dulloo, Piyushika; Rupachandra, S; Jagadeeshwari, S; M, Joann Davina; Porkodi, S

doi:10.22159/ajpcr.2018.v11i3.23009

Cited by 4 publications

(3 citation statements)

References 27 publications

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“…Extendedspectrum B-lactamase produced by Escherichia coli, Enterobacter aerogenes, Klebsiella pneumoniae, and Hafnia alvei were isolated from urinary tract infection and subjected to double disc diffusion assay using three solvent extracts [15]. The inhibition was significant with hexane extract and can be given along with other antibiotics as new alternative treatment for urinary tract infection [16]. The methanolic, chloroform, and hexane extracts of V. jatamansi were evaluated for their antimicrobial effect against strains Staphylococcus aureus, Pseudomonas aeruginosa, Micrococcus luteus, E. coli, Salmonella ebony, Lactobacillus plantarum, and Staphylococcus epidermidis.…”

Section: Antibacterial Activitymentioning

confidence: 99%

Valeriana Jatamansi: An Ethnobotanical Review

Patan

2018

Asian J Pharm Clin Res

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Objective:The main objective of review is an effort to give detailed information on Valeriana jatamansi and its multiple medicinal properties. Methods:As per the literature, the plant V. jatamansi is a perennial herb and found at subtemperate Himalayan regions of the Kumaon and Garhwal. The plant most popularly known as "Valerian." The plant belongs to the family Valerianaceae. The roots and rhizomes of the plants are traditionally used to treat ulcers, jaundice, dry cough, seminal weakness, skin diseases, leprosy, and for sleep enhancement due to the presence of a variety of different chemical constituents such as sesquiterpenes, coumarins, iridoids, lignanoids, alkaloids, and flavonoids. From literature, the reported methods performed for different pharmacological activities have been thoroughly discussed and complied. Results:Most of the studies compiled from literature shown that V. jatamansi possess antimicrobial, antioxidant, anti-inflammatory, cytotoxic, anxiolytic, and neuroprotective activities. Conclusion:The review reveals that continuous research on this plant is needed to know the exact molecular mechanism and further elaborative studies can lead to develop safe therapeutic use in modern medicine.

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Section: Antibacterial Activitymentioning

confidence: 99%

Valeriana Jatamansi: An Ethnobotanical Review

Patan

2018

Asian J Pharm Clin Res

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“…Senna alata Linn., Senna alexandrina Mill., and Senna occidentalis (L.) Link (Leguminosae) are three plant species of Senna that possess multiple medicinal properties including antimicrobial, antidepressant, antianxiety, and antibacterial (Sermakkani & Thangapandian, 2012; Khare et al, 2017; Harsha et al, 2018). Senna leaves and pods contain sennosides A, B, C, D, and G, rhein, kaemferin, and isorhein in free and compound glycoside form (Srivastava et al, 1983).…”

Section: Introductionmentioning

confidence: 99%

Functional Imaging of Neurotransmitters in Hymenolepis diminuta Treated with Senna Plant Through Light and Confocal Microscopy

2018

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Previous studies have shown the anthelmintic efficacy of Senna alata, Senna alexandrina and Senna occidentalis on the zoonotic parasite Hymenolepis diminuta through microscopic studies on morphological structure. The present study is based on the light and confocal microscopic studies to understand if Senna extracts affect neurotransmitter activity of the parasites. A standard concentration (40 mg/mL) of the three leaf extracts and one set of 0.005 mg/mL concentration of the reference drug praziquantel were tested against the parasites, keeping another set of parasites in phosphate buffer saline as a control. Histochemical studies were carried out using acetylthiocholine iodide as the substrate and acetylcholinesterase as the marker enzyme for studying the expression of the neurotransmitter of the parasite and the staining intensity was observed under a light microscope. Immunohistochemical studies were carried out using anti serotonin primary antibody and fluorescence tagged secondary antibody and observed using confocal microscopy. Intensity of the stain decreases in treated parasites compared with the control which implies loss of activity of the neurotransmitters. These observations indicated that Senna have a strong anthelmintic effect on the parasite model and thus pose as a potential anthelmintic therapy.

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“…Senna plant constitutes many species of shrubs and trees, most of which possess a rich source of secondary metabolites used for medicinal purposes and also for developing economically sound folklore remedies [6][7][8]. Three species of Senna plants viz.…”

mentioning

confidence: 99%

Senna Plant Induces Disruption on the Mitochondria of Hymenolepis Diminuta

Ukil

Roy

Nandi

et al. 2018

Int J Pharm Pharm Sci

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Objective: The present study aims at observing the effects of three species of Senna plants, viz. Senna alata, S. alexandrina and S. occidentalis on the ultrastructure of the mitochondria of the tapeworm, Hymenolepis diminuta.Methods: Worms were treated with leaf extracts of the three plant species with a standard dose concentration of 40 mg/ml and keeping one group of parasites in phosphate buffer saline (PBS) as a control. The parasites from control and treated medium were simultaneously removed after the loss of motility and fixed in 3% gluteraldehyde. They were processed for ultramicrograph observations of the worm's mitochondria with special reference to shape and cytoplasm through transmission electron microscopy (TEM). Results:The study showed loss of architecture in the outer mitochondrial membrane. The inner membrane became distorted with inconspicuous cristae and matrix became lucent in all plant treated worms compared to control. Amongst the three plants, S. alexandrina showed overall distortion in the shape leading to bloating of mitochondria. Conclusion:The observations depict pronounced alterations in the structure of mitochondria, thus signifying depletion of energy synthesis in the parasite. Senna plant could, therefore, be a potent anthelmintic alternative.

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Isolation and Identification of Antimicrobial Proteins From the Leaves of Valeriana Hardwickii and Senna Obtusifolia

Cited by 4 publications

References 27 publications

Valeriana Jatamansi: An Ethnobotanical Review

Valeriana Jatamansi: An Ethnobotanical Review

Functional Imaging of Neurotransmitters in Hymenolepis diminuta Treated with Senna Plant Through Light and Confocal Microscopy

Senna Plant Induces Disruption on the Mitochondria of Hymenolepis Diminuta

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