Isolation and identification of antifungal peptides from Bacillus amyloliquefaciens W10

Zhang, Qingxia; Zhang, Ying; Shan, Hai-Huan; YunHui, Tong; Chen, Xijun; Liu, Fengquan

doi:10.1007/s11356-017-0179-8

Cited by 41 publications

(23 citation statements)

References 38 publications

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“…All the studies reported the antibiosis of B. cinerea by the NRPs metabolites produced by B. subtilis but do not demonstrate directly the implications of these molecules in the antibiosis ( Tareq et al, 2014 ; Farace et al, 2015 ; Wang et al, 2015 ; Arroyave-Toro et al, 2017 ). The same occurs with B. amyloliquefaciens ( Ji et al, 2013 ; Pretorius et al, 2015 ; Tanaka et al, 2015 ; Zhang et al, 2017 ), B. marinus ( Gu et al, 2017 ), B. atrophaeus ( Zhang et al, 2013 ), and B. velezensis ( Ge et al, 2016 ; Romero et al, 2016 ; Gao et al, 2017 ).…”

Section: Discussionmentioning

confidence: 63%

“…With respect to the activity of lipopeptides, Romano et al (2013) reported the non-effect of B. amyloliquefaciens lipopeptides against B. cinerea at concentrations of 0.1 mg mL -1 . Tareq et al (2014) established a range of lipopeptide activity in B. subtilis between 0.5 to 300 μg mL -1 and Zhang et al (2017) determined the maximum activity of lipopeptides of B. amyloliquefaciens at 30 mg mL -1 . Our study tested a range of concentrations from 0.5 to 20 mg mL -1 and confirmed the antifungal activity of XT1 lipopeptides with inhibitory and fungicidal effects of these compounds at concentrations as low as 8 mg mL -1 (MIC and MFC).…”

Section: Discussionmentioning

confidence: 99%

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Antifungal Activity of Lipopeptides From Bacillus XT1 CECT 8661 Against Botrytis cinerea

Toral¹,

et al. 2018

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This work aims to explore the capacity of a Bacillus methylotrophicus (later heterotypic synonym of Bacillus velezensis) strain named XT1 CECT 8661 against the necrotrophic plant pathogen Botrytis cinerea and to identify the compounds responsible for its activity. Q_TOF electrospray mass spectrometry analysis allows us to detect several lipopeptides – surfactin, bacillomycin, and fengycin – in XT1 cultures. In vitro antibiosis studies demonstrated the efficiency of the lipopeptide fraction for the inhibition of fungal growth. In fact, microscopy studies (SEM/TEM) revealed, an alteration of the morphology of the phytopathogen in interaction with lipopeptides, with resistance structures appearing in the early stages of growth of the fungus. Our studies, carried out with tomatoes, grapes, and strawberries have demonstrated the efficiency of Bacillus XT1 CECT 8661 lipopeptides against B. cinerea infection and it capability to trigger the antioxidant activity in fruit. Overall, the results of this study highlight the potential of lipopeptides of this strain as an effective biological control agent against the colonisation of B. cinerea.

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Section: Discussionmentioning

confidence: 63%

Section: Discussionmentioning

confidence: 99%

Antifungal Activity of Lipopeptides From Bacillus XT1 CECT 8661 Against Botrytis cinerea

Toral¹,

et al. 2018

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“…These molecules insert themselves into the microbial cell membranes, or chelating cations. This effect alters the membrane permeability, eventually disrupting it and causing cell swelling and death ( Zhang et al, 2017 ; Zhao et al, 2017 ).…”

Section: Methods For Controlling Biofilm Formation In the Food Industmentioning

confidence: 99%

Biofilms in the Food Industry: Health Aspects and Control Methods

et al. 2018

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Diverse microorganisms are able to grow on food matrixes and along food industry infrastructures. This growth may give rise to biofilms. This review summarizes, on the one hand, the current knowledge regarding the main bacterial species responsible for initial colonization, maturation and dispersal of food industry biofilms, as well as their associated health issues in dairy products, ready-to-eat foods and other food matrixes. These human pathogens include Bacillus cereus (which secretes toxins that can cause diarrhea and vomiting symptoms), Escherichia coli (which may include enterotoxigenic and even enterohemorrhagic strains), Listeria monocytogenes (a ubiquitous species in soil and water that can lead to abortion in pregnant women and other serious complications in children and the elderly), Salmonella enterica (which, when contaminating a food pipeline biofilm, may induce massive outbreaks and even death in children and elderly), and Staphylococcus aureus (known for its numerous enteric toxins). On the other hand, this review describes the currently available biofilm prevention and disruption methods in food factories, including steel surface modifications (such as nanoparticles with different metal oxides, nanocomposites, antimicrobial polymers, hydrogels or liposomes), cell-signaling inhibition strategies (such as lactic and citric acids), chemical treatments (such as ozone, quaternary ammonium compounds, NaOCl and other sanitizers), enzymatic disruption strategies (such as cellulases, proteases, glycosidases and DNAses), non-thermal plasma treatments, the use of bacteriophages (such as P100), bacteriocins (such us nisin), biosurfactants (such as lichenysin or surfactin) and plant essential oils (such as citral- or carvacrol-containing oils).

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“…The structure of the peptide cycle combines with a long fatty acid chain to produce an amphiphilic trait, which determines the most suitable target sites on the cellular membranes(10). In addition, due to its variety and the number of amino acids as well as the diversity of fatty acid chain length, CLPs have multiple homologs(11). Moreover, CLPs derived from Bacillus spp.…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, CLPs derived from Bacillus spp. can be classified into three main subfamilies: iturin, surfactin, and fengycin(11–13). Interestingly, surfactin has powerful antiviral activities but low activities against bacteria and fungi(14), while iturin and fengycin exhibit an obvious antifungal activity against a range of filamentous fungi(9, 15).…”

Section: Introductionmentioning

confidence: 99%

Inhibitory effect ofBacillus subtilisWL-2 and its IturinA lipopeptides againstPhytophthora infestans

Wang

Zhang

et al. 2019

Preprint

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15Potato late blight triggered by Phytophthora infestans ((Mont.) de Bary) represents a 16 great food security threat worldwide and is difficult to control. Currently, Bacillus spp. 17 have been considered biocontrol agents to control many fungal diseases. Here, 18 Bacillus subtilis WL-2 was selected as the antifungal strain with the most potential 19 against P. infestans mycelium growth. Additionally, the functional metabolites 20 extracted from WL-2 were identified as IturinA-family cyclic lipopeptides (CLPs) via 21 2 high-performance liquid chromatography (HPLC) and electrospray ionization mass 22 spectrometry (ESI-MS). Analyses using scanning and transmission electron 23 microscopy (SEM and TEM) revealed that IturinA caused a change in the mycelial 24 surface and damage to the internal cell structure, including cell membrane disruption 25 and irregular organelle formation. Moreover, propidium iodide staining and nucleic 26 acid and protein release were detected to clarify the cell membrane damage caused by 27 IturinA. Additionally, IturinA triggered reactive oxygen species (ROS) generation and 28 malondialdehyde (MDA) production. Mitochondrial membrane potential (MMP), 29 mitochondrial respiratory chain complexes activity (MRCCA), respiratory control rate 30 (RCR), and oxidative phosphorylation efficiency (P/O) assays indicated that P. 31 infestans mitochondria affected by IturinA were so seriously damaged that the MMP 32 and MRCCA declined remarkably and that mitochondrial ATP production ability was 33 weakened. Therefore, IturinA induces cell membrane damage, oxidative stress, and 34 dysfunction of mitochondria, resulting in P. infestans hyphal cell death. As such, the 35 results highlight that B. subtilis WL-2 and IturinA have great potential as candidates for 36 inhibiting P. infestans mycelium growth and controlling potato late blight. 37 IMPORTANCE 38Potato (Solanum tuberosum L.) is the fourth most common global food crop, and 39 its planting area and yield increase yearly. Notably, in 2015, China initiated a potato 40 staple food conversion strategy, and by 2020, approximately 50% of potatoes will 41 be consumed as a staple food. The plant pathogen fungus Phytophthora infestans 42 ((Mont.) de Bary) is the culprit of potato late blight; however, biological agents 43 3 rather than chemicals are highly necessary to control this threatening disease. In 44 this study, we discovered an antifungal substance, IturinA, a lipopeptide produced 45 by Bacillus subtilis WL-2. Moreover, our research revealed the actual mechanism 46 of IturinA against P. infestans mycelium growth and clarified the potential of B. 65Behind rice, wheat, and corn, potato (Solanum tuberosum L.) is the fourth most 66 stable food crop in the world. However, potato production is often endangered by 67 many pathogens. Worryingly, late blight triggered by Phytophthora infestans ((Mont.) 68 de Bary) could directly reduce or even eliminate potato production, and an outbreak 69 of this disease could even result in a grievous ...

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Isolation and identification of antifungal peptides from Bacillus amyloliquefaciens W10

Cited by 41 publications

References 38 publications

Antifungal Activity of Lipopeptides From Bacillus XT1 CECT 8661 Against Botrytis cinerea

Antifungal Activity of Lipopeptides From Bacillus XT1 CECT 8661 Against Botrytis cinerea

Biofilms in the Food Industry: Health Aspects and Control Methods

Inhibitory effect ofBacillus subtilisWL-2 and its IturinA lipopeptides againstPhytophthora infestans

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