Isolation and identification of angiogenesis-promoting components in Huanglian Jiedu decoction using live cell bio-specific extraction

Mass spectrometry-based metabolomics for discovering active ingredients and exploring action mechanism of herbal medicine

“…The results showed that rhein, astragaloside IV, emodin, chrysophanol and other compounds may be the pharmacodynamic basis of KLX. Liao et al (2023) isolated and identified the specific binding components of Huanglian Jiedu Decoction to human umbilical vein endothelial cells by a combination of UPLC-Orbitrap-MS and found 13 active components such as geniposide, thapsigin, baicalin, and berberine as pharmacodynamic components that promote angiogenesis. Gao et al (2023) isolated and identified 45 compounds from the leaves of Amomumvillosum Lour., with the help of HPLC-QTOF-MS technology and further experimented that 14 compounds (one unknown compound and thirteen known compounds) could prevent and treat diseases caused by inflammation and oxidative stress.…”

Screening of the active Ingredients in Huanglian Jiedu decoction through amide bond-Immobilized magnetic nanoparticle-assisted cell membrane chromatography

“…Moreover, the high accumulation of reactive oxygen species is closely related to glutamate-induced neuronal damage (Pan et al, 2020;Tonsomboon et al, 2021). The Huanglian Jiedu decoction (HLJDD), which originates from "Arcane Essentials from the Imperial Library", is composed of Coptis chinensis Franch (Ranunculaceae; Coptidis Rhizoma), Scutellaria baicalensis Georgi (Lamiaceae; Scutellariae Radix), Phellodendron amurense Rupr (Rutaceae; Phellodendri Amurensis cortex), and Gardenia jasminoides Ellis (Rubiaceae; Gardeniae fructus) in a ratio of 3:2:2:3 (He et al, 2022a;He et al, 2022b;Liao et al, 2022). As a Chinese classical formula with heat-clearing and detoxicating effects, HLJDD has been proved to have the potential to treat AD by many modern pharmacological studies (Sun et al, 2018;Wu et al, 2020).…”