Isolation and identification of an anti-hepatitis B virus compound from Hydrocotyle sibthorpioides Lam

Huang, Quanfang; Zhang, Shijun; Huang, Renbin; Wei, Ling; Chen, Yongxing; Lv, Shujuan; Liang, Chunhong; Tan, Sheng; Liang, Shuang; Zhuo, Lang; Lin, Xiaobo

doi:10.1016/j.jep.2013.09.009

Cited by 46 publications

(28 citation statements)

References 24 publications

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“…It is reported that it is efficient in the treatment of rheumatalgia, dysentery, jaundice, and exerted a potent inhibitory effect on the growth of tumours [9]. Recently, the in vitro and in vivo antiviral properties of H. sibthorpioides against hepatitis B virus replication have been demonstrated [10]. Moreover, this plant is been used in different traditional cuisines of Assam.…”

Section: Introductionmentioning

confidence: 99%

Acute oral toxicity evaluation of extracts of Hydrocotyle sibthorpioides in wister albino rats as per OECD 425 TG

et al. 2019

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Section: Introductionmentioning

confidence: 99%

Acute oral toxicity evaluation of extracts of Hydrocotyle sibthorpioides in wister albino rats as per OECD 425 TG

et al. 2019

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“…Over the last 20 years, CHM were widely used in the treatment of HBV carriers and some of the herbs including Radix Astragali, Radix Salviae miltiorrhizae and Radix Bupleuri, have been proved to be effective in terms of seroreversion of HBsAg, HBeAg and HBV DNA [28]. Recently, a number of natural compounds from CHM that block HBV replication have been identified, including epigallocatechin-3-gallate [29], curcumin [30], wogonin [31], ellagic acid [32], artemisinin and artesunate [33], chrysophanol 8-O-β-d-glucoside [34], SSc [18], asiaticoside [35], helioxanthin [36], and oxymatrine [37]. In this study we investigated the anti-HBV activity of SSc, one of the saikosaponins extracted from the medicinal plant Radix Bupleuri.…”

Section: Discussionmentioning

confidence: 99%

Saikosaponin C exerts anti-HBV effects by attenuating HNF1α and HNF4α expression to suppress HBV pgRNA synthesis

Pan

et al. 2019

Inflamm. Res.

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Objective Saikosaponin c (SSc), a compound purified from the traditional Chinese herb of Radix Bupleuri was previously identified to exhibit anti-HBV replication activity. However, the mechanism through which SSc acts against HBV remains unknown. In this study, we investigated the mechanism of SSc mediated anti-HBV activity. Methods HepG2.2.15 cells were cultured at 37 ℃ in the presence of 1-40 μg/mL of SSc or DMSO as a control. The expression profile of HBV markers, cytokines, HNF1α and HNF4α were investigated by real-time quantitative PCR, Elisa, Western blot and Dot blotting. Knockdown of HNF1α or HNF4α in HepG2.2.15 cells was mediated by two small siRNAs specifically targeting HNF1α or HNF4α. Results We found that SSc stimulates IL-6 expression, leading to attenuated HNF1α and HNF4α expression, which further mediates suppression of HBV pgRNA synthesis. Knockdown of HNF1α or HNF4α in HepG2.2.15 cells by RNA interference abrogates SSc's anti-HBV role. Moreover, SSc is effective to both wild-type and drug-resistant HBV mutants. Conclusion SSc inhibits pgRNA synthesis by targeting HNF1α and HNF4α. These results indicate that SSc acts as a promising compound for modulating pgRNA transcription in the therapeutic strategies against HBV infection.

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“…Recently, H. sibthorpioides was shown to exhibit antiviral activity against hepatitis B virus [8]. In this study, the potential use of this plant to inhibit in vitro DENV-2 replication was investigated.…”

Section: Discussionmentioning

confidence: 99%

“…Another important use of H. sibthorpioides in traditional Chinese medicine is to treat hepatitis [7]. Recently, the in vitro and in vivo antiviral properties of H. sibthorpioides against hepatitis B virus replication have been demonstrated [8]. However, the antiviral effect of this plant against other viruses has not been investigated despite its folkloric use.…”

Section: Introductionmentioning

confidence: 99%

The Effect ofHydrocotyle sibthorpioidesLam. Extracts onIn VitroDengue Replication

Husin

Yean

Gan³

et al. 2015

Evidence-Based Complementary and Alternative Medicine

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Objective. To investigate the potential effect of Hydrocotyle sibthorpioides Lam. (H. sibthorpioides) extracts against in vitro dengue viral replication. Methods. The cytotoxicity of H. sibthorpioides was evaluated using a cell viability assay. Cells were pre- and posttreated with water and methanol extracts of H. sibthorpioides, and the viral inhibitory effect was investigated by observing the morphological changes, which were further confirmed by plaque assay. Results. The methanolic extract cytotoxicity was higher in Vero and C6/36 cells than the cytotoxicity of the water extract. Preincubation of the cells with H. sibthorpioides extract showed nonexistent to mild prophylactic effects. The posttreatment of Vero cells with H. sibthorpioides methanolic extract presented higher antidengue activities when compared with the water extract. Surprisingly, posttreatment of C6/36 cells resulted in an enhancement of viral replication. Conclusion. H. sibthorpioides had variable effects on dengue viral replication, depending on the treatment, cell lines, and solvent types. This study provides important novel insights on the phytomedicinal properties of H. sibthorpioides extracts on dengue virus.

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Isolation and identification of an anti-hepatitis B virus compound from Hydrocotyle sibthorpioides Lam

Cited by 46 publications

References 24 publications

Acute oral toxicity evaluation of extracts of Hydrocotyle sibthorpioides in wister albino rats as per OECD 425 TG

Acute oral toxicity evaluation of extracts of Hydrocotyle sibthorpioides in wister albino rats as per OECD 425 TG

Saikosaponin C exerts anti-HBV effects by attenuating HNF1α and HNF4α expression to suppress HBV pgRNA synthesis

The Effect ofHydrocotyle sibthorpioidesLam. Extracts onIn VitroDengue Replication

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