Isolation and Identification of an Alkaloid Compound from Bebuas Leaves (Premna serratifolia) as an Anti-Inflammatory in White Rats (Rattus norvegicus)
Abstract:Bebuas (Premna serratifolia) traditionally is used by Malay people as a traditional medicine to restore health. This indicates it contains chemicals that potentially have an anti-inflammatory activity. The results of phytochemical screening showed that the ethanol extract contains phenolics, alkaloids, flavonoids, steroids and saponins. Meanwhile, hexane extract only contains steroids. Anti-inflammatory activity evaluation on crude extracts showed that the ethanol extract was more active than the hexane extrac… Show more
“…Meanwhile, the water fraction shows detection for alkaloids, flavonoids, and saponins. The solubility of alkaloids in water is due to their ionic form, making them more soluble in water than in organic solvents (14). Flavonoids, typically polar in nature, are also detected in the water fraction (15).…”
Section: Phytochemicals In Extract and Fractionsmentioning
Erectile dysfunction (ED) and premature ejaculation are prevalent male sexual dysfunctions affecting various age groups. This study explores the potential aphrodisiac effects of Clove leaves (Syzygium aromaticum L.), a plant rich in secondary metabolites, including alkaloids, flavonoids, saponins, and steroids. Fractionation of the ethanol extract yielded n-hexane, ethyl acetate, and water fractions. Phytochemical screening revealed the presence of alkaloids, flavonoids, and saponins in the water fraction, while steroids were detected in the ethanol extract, ethyl acetate, and n-hexane fractions. In vivo tests on male rats demonstrated that the ethanol extract, n-hexane, and ethyl acetate fractions significantly reduced mounting latency (ML), increased mounting frequency (MF), and accelerated intromission latency (IL), indicating heightened sexual arousal and endurance. Moreover, these fractions delayed ejaculatory latency (EL) and increased ejaculation frequency (EF), akin to the positive control, X-Gra. Notably, the n-hexane fraction showed the closest efficacy to X-Gra. The presence of steroids in the active fractions suggests their role in eliciting aphrodisiac effects. Further isolation and purification of the active compound(s) may optimize therapeutic outcomes. This study underscores the potential of Clove leaf fractions as natural aphrodisiacs, warranting further investigation for clinical applications.
“…Meanwhile, the water fraction shows detection for alkaloids, flavonoids, and saponins. The solubility of alkaloids in water is due to their ionic form, making them more soluble in water than in organic solvents (14). Flavonoids, typically polar in nature, are also detected in the water fraction (15).…”
Section: Phytochemicals In Extract and Fractionsmentioning
Erectile dysfunction (ED) and premature ejaculation are prevalent male sexual dysfunctions affecting various age groups. This study explores the potential aphrodisiac effects of Clove leaves (Syzygium aromaticum L.), a plant rich in secondary metabolites, including alkaloids, flavonoids, saponins, and steroids. Fractionation of the ethanol extract yielded n-hexane, ethyl acetate, and water fractions. Phytochemical screening revealed the presence of alkaloids, flavonoids, and saponins in the water fraction, while steroids were detected in the ethanol extract, ethyl acetate, and n-hexane fractions. In vivo tests on male rats demonstrated that the ethanol extract, n-hexane, and ethyl acetate fractions significantly reduced mounting latency (ML), increased mounting frequency (MF), and accelerated intromission latency (IL), indicating heightened sexual arousal and endurance. Moreover, these fractions delayed ejaculatory latency (EL) and increased ejaculation frequency (EF), akin to the positive control, X-Gra. Notably, the n-hexane fraction showed the closest efficacy to X-Gra. The presence of steroids in the active fractions suggests their role in eliciting aphrodisiac effects. Further isolation and purification of the active compound(s) may optimize therapeutic outcomes. This study underscores the potential of Clove leaf fractions as natural aphrodisiacs, warranting further investigation for clinical applications.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.