Isolation and Identification of Adherent Gram-Negative Microorganisms from Four Meat-Processing Facilities

Hood, Scott; Zottola, E. A.

doi:10.4315/0362-028x-60.9.1135

Cited by 45 publications

(29 citation statements)

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“…Ideally, this will allow studies on biofilms that are more relevant than those produced using laboratory models. The approach has been used to compare hygienic properties of different materials (Guobjornsdottir et al 2005), to identify microbiota in food production factories (Hood and Zottola 1997;Mettler and Carpentier 1998;Gunduz and Tuncel 2006), and to detect biofilm formation (Holah et al 1989;Gibson et al 1995), and recently, we used this approach to study the effect of kitchen cleaning methods (Røssvoll et al 2015). However, little has been done to evaluate how well the microbiota developed by this approach reflects the microbiota developed in situ, where the surfaces can be of a different quality and (or) condition than the coupons used.…”

Section: Introductionmentioning

confidence: 99%

Microbiota formed on attached stainless steel coupons correlates with the natural biofilm of the sink surface in domestic kitchens

et al. 2016

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Stainless steel coupons are frequently used in biofilm studies in the laboratory, as this material is commonly used in the food industry. The coupons are attached to different surfaces to create a "natural" biofilm to be studied further in laboratory trials. However, little has been done to investigate how well the microbiota on such coupons represents the surrounding environment. The microbiota on sink wall surfaces and on new stainless steel coupons attached to the sink wall for 3 months in 8 domestic kitchen sinks was investigated by nextgeneration sequencing (MiSeq) of the 16S rRNA gene derived from DNA and RNA (cDNA), and by plating and identification of colonies. The mean number of colony-forming units was about 10-fold higher for coupons than sink surfaces, and more variation in bacterial counts between kitchens was seen on sink surfaces than coupons. The microbiota in the majority of biofilms was dominated by Moraxellaceae (genus Moraxella/Enhydrobacter) and Micrococcaceae (genus Kocuria). The results demonstrated that the variation in the microbiota was mainly due to differences between kitchens (38.2%), followed by the different nucleic acid template (DNA vs RNA) (10.8%), and that only 5.1% of the variation was a result of differences between coupons and sink surfaces. The microbiota variation between sink surfaces and coupons was smaller for samples based on their RNA than on their DNA. Overall, our results suggest that new stainless steel coupons are suited to model the dominating part of the natural microbiota of the surrounding environment and, furthermore, are suitable for different downstream studies.Key words: microbiota, stainless steel coupons, sink surface, domestic kitchens. Résumé :Les études sur les biofilms utilisent couramment des coupons en acier inoxydable en laboratoire puisque ce matériau est répandu dans l'industrie alimentaire. Ces coupons sont attachés à diverses surfaces afin de créer un biofilm « naturel » aux fins d'études plus approfondies en laboratoire. Or, on s'est peu demandé à quel point la microflore retrouvée sur de tels coupons est représentative de l'environnement avoisinant. On a examiné la microflore de nouveaux coupons d'acier inoxydable attachés à la paroi de l'évier pendant trois mois, ainsi que celle des parois de l'évier comme telles, au moyen du séquençage de prochaine génération (MiSeq) du gène de l'ARNr 16S issu d'ADN et d'ARN (ADNc), et par ensemencement et identification des colonies. Le nombre moyen d'unités formant colonies était environ 10 fois plus élevé sur les coupons que sur les surfaces de l'évier, et on a observé davantage de variations des numérations bactériennes selon les cuisines dans le contexte des surfaces d'éviers que des coupons. La microflore de la majorité des biofilms était dominée par les Moraxellaceae (genre Moraxella/ Enhydrobacter) et les Micrococcaceae (genre Kocuria). Les résultats ont démontré que la variation de la microflore était principalement attribuable aux différences entre les cuisines (38,2 %), suivie par les ...

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Section: Introductionmentioning

confidence: 99%

Microbiota formed on attached stainless steel coupons correlates with the natural biofilm of the sink surface in domestic kitchens

et al. 2016

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“…(Table 2) were found on the surface of SS coupons at trolley #1, soup tank (bottom) and soup filling table as an evident to support the biofilm formation. Pseudomonas has been found to adhere easily to the surfaces and is excellent biofilm formers as reported in several studies [28,29].…”

Section: Resultsmentioning

confidence: 91%

Adhesive Microflora on Stainless Steel Coupons in Seafood Processing Plant

Hoa

Mahakarnchanakul²,

Sajjaanantakul³

et al. 2015

JFNS

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Abstract:Adhering microflora profile is essential for the development and design of cleaning and sanitizing procedures, which is a part of the HACCP system. The profile provides a complete view of the target microorganisms that can potentially contaminate food products. The aim of this study is, therefore, focused on examination of microbial population adhered to the equipment surfaces at seafood processing plant. Adhering microflora was evaluated by installing the stainless steel coupons on the surface of equipment. Seventy stainless steel coupons were attached on 7 different locations at seafood processing plant and then fourteen coupons were collected at 1, 2, 3, 5 and 9 weeks interval. The population of bacteria adhered on samples were enumerated using swab test and spread plate method on different selective microbiological media. The total viable count and Pseudomonas spp. population found on the stainless steel coupons were ranged from 10 1 to 10 6 cfu/cm 2 . The results also indicated that mature biofilm might form at some locations after three weeks. After 5 weeks, the highest viable bacterial population (10 6 cfu/cm 2 ) was observed on the stainless steel coupons attached in the trolley used for carrying the soup. These finding supported that the appropriate cleaning and sanitizing procedure should be strictly applied to assure safety in food processing plant.

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“…McGuire & Swartzel [1989] demonstrated that primary adhesion of bacteria to stainless steel and Tefl on was enhanced in the presence of surface-associated milk proteins. According to Hood & Zottola [1997], the material conditioned by diluted milk was better for attachment of pathogens than the whole milk. It was as- sumed that some molecules, like bovine serum album (BSA), inhibited cells attachment process to solid surfaces [Hood & Zottola, 1997].…”

Section: Solid Surface Propertiesmentioning

confidence: 99%

“…According to Hood & Zottola [1997], the material conditioned by diluted milk was better for attachment of pathogens than the whole milk. It was as- sumed that some molecules, like bovine serum album (BSA), inhibited cells attachment process to solid surfaces [Hood & Zottola, 1997].…”

Section: Solid Surface Propertiesmentioning

confidence: 99%

Bacterial Biofilms on Food Contact Surfaces - a Review

Myszka¹,

Czaczyk²

2011

Pol. J. Food Nutr. Sci.

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This review will discuss some of the basic concepts concerning biofi lm formation, development and control in the food industry. Biofi lm formation process on food contact surfaces can have a detrimental effect on the microbial status of food. The presence of biofi lm on abiotic materials can contaminate the product through direct contact. As a consequence, food spoilage is likely to occur that may lead to reduced shelf life and increased risk of food poisoning from pathogens. Bacteria colonizing food processing surfaces are extremely diffi cult to eradicate. Biofi lms can tolerate antimicrobial agents at concentrations of 10-1000 times that needed to inactivate genetically equivalent planktonic bacteria. A better understanding of bacterial adhesion process is needed for the production of microbiologically-safe and good-quality products in the food industry.

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Isolation and Identification of Adherent Gram-Negative Microorganisms from Four Meat-Processing Facilities

Cited by 45 publications

References 0 publications

Microbiota formed on attached stainless steel coupons correlates with the natural biofilm of the sink surface in domestic kitchens

Microbiota formed on attached stainless steel coupons correlates with the natural biofilm of the sink surface in domestic kitchens

Adhesive Microflora on Stainless Steel Coupons in Seafood Processing Plant

Bacterial Biofilms on Food Contact Surfaces - a Review

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