Isolation and identification of Acanthamoeba strains from soil and tap water in Yanji, China

Abstract

BackgroundMembers of the genus Acanthamoeba are widely distributed throughout the world, and some of them are considered pathogenic, as they are capable of causing corneal and central nervous system diseases. In this study, we isolated Acanthamoeba strains from soil and tap water in Yanji, China.MethodsWe identified four strains of Acanthamoeba (CJY/S1, CJY/S2, CJY/S3, and CJY/W1) using mitochondrial DNA restriction fragment length polymorphism (mtDNA RFLP) analysis. Nuclear 18S rDNA sequences were used for phylogenetic analysis and species identification.ResultsGenotypic characterization of the isolates showed that they belonged to genotypes T4 (CJY/S1 and CJY/S2), T5 (CJY/S3), and T16 (CJY/W1). Sequence differences between CJY/S1 and Acanthamoeba castellanii Neff, CJY/S2 and Acanthamoeba KA/E7, and CJY/S3 and Acanthamoeba lenticulata 68–2 were 0.31, 0.2, and 0.26%, respectively. 18S ribosomal deoxyribonucleic acid (rDNA) of CJY/W1 had 99% sequence identity to that of Acanthamoeba sp. U/H-C1. Strains CJY/S1 and CJY/S2, isolated from soil, had similar mtDNA RFLP patterns, whereas strain CJY/W1, isolated from tap water, displayed a different pattern.ConclusionsTo the best of our knowledge, this is the first report on the identification of genotypes T4, T5, and T16 from environmental sources in Yanji, China.

Genotyping and interaction-reality of Acanthamoeba, enteric adenovirus and rotavirus in drinking water, Egypt.

“…Furthermore, other genotypes such as T5, T15, T3 and T9 were identified during this work (Figures 1-3). Our findings are completely in agreement with the previous reports demonstrated that various Acanthamoeba genotypes isolated from different countries such as T3 and T5 were isolated from swimming pools in Egypt (Morsy et al, 2016), T1-4 and T7 were isolated from freshwater in Egypt (Lorenzo-Morales et al, 2006), T3 and T4 were isolated from water in France (Ovrutsky et al, 2013), T3-5 and T11 were isolated from tap water in Iran (Niyyati et al, 2015) and T4, T5 and T16 were isolated from soil and tap water in China (Xuan et al, 2017).…”

Adaption of microbial communities to the hostile environment in the Doce River after the collapse of two iron ore tailing dams

“…All these data indicate that this genus may at least tolerate the toxic environmental condition imposed on the Doce River. Like other species of Acanthamoeba, A. lenticulata have been isolated from or detected in different environmental samples in previous studies ( Sente et al., 2016 ; Fallah et al., 2017 ; Spotin et al., 2017 ; Xuan et al., 2017 ; Basher et al., 2018 ). However, this work seems to be the first report of isolation if this amoeba species from a profoundly impacted environment.…”

“…In their natural environment, these organisms feed on bacteria and particulate matters. Most of these studies report this genus as a risk to human health when its species are occurring in water bodies and drinking water ( Sente et al., 2016 ; Armand et al., 2016 ; Fallah et al., 2017 ; Spotin et al., 2017 ; Xuan et al., 2017 ; Basher et al., 2018 ). Acanthamoeba is described as the most common FLA genus in different aquatic environments ( Magnet et al., 2013 ) and its prevalence is dependent on the water temperature and turbidity ( Ren et al., 2018 ).…”

Ramifications of the diverse mRNA patterns in Acanthamoeba royreba

“…Therefore, could these observations indicate that this amoebae exists as an endosymbiont in placental or tumoral tissue? Could it possibly play a crucial role in the womb becoming an immunologic sanctuary 13 that permits the presence of a foreign fetus without rejection? Can this unusual property be extrapolated to explain the tolerance of the immune system to fetal or tumoral tissue?…”