Isolation and identification of a radical scavenging antioxidant – punicalagin from pith and carpellary membrane of pomegranate fruit

Kulkarni, Anand; Aradhya, Somaradhya Mallikarjuna; Divakar, Soundar

doi:10.1016/j.foodchem.2004.01.006

Cited by 180 publications

(108 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Pomegranate juice was also the most active antioxidant sample that was tested. It is noteworthy that among the polyphenols present in PJ, punicalagin is the most potent antioxidant ingredient contributing largely to the observed antioxidant properties of PJ, as previously reported [7,24].…”

Section: Discussionsupporting

confidence: 62%

“…Pomegranate juice and extracts have been shown to have potent in vitro antioxidant [7,24] and in vivo anti-atherosclerotic properties [8,9,15], attributed to its high content of polyphenols including ETs and EA. Recently, there have also been numerous reports on the in vitro and in vivo anticancer properties of pomegranates [10,[25][26][27][28][29][30].…”

Section: Discussionmentioning

confidence: 99%

“…Recently, there have also been numerous reports on the in vitro and in vivo anticancer properties of pomegranates [10,[25][26][27][28][29][30]. The major pomegranate ET, punicalagin, is reported as the active ingredient responsible for N 50% of the juice's antioxidative potential [7,24] and can reach levels of N2 g/L of juice [7]. However, the synergistic and/or additive effects of the individual purified polyphenols present in PJ and also in a wellstandardized extract form are yet to be evaluated for antiproliferative and apoptotic activities.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

In vitro antiproliferative, apoptotic and antioxidant activities of punicalagin, ellagic acid and a total pomegranate tannin extract are enhanced in combination with other polyphenols as found in pomegranate juice

Seeram

Adams

Henning

et al. 2005

The Journal of Nutritional Biochemistry

954

664

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 62%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

In vitro antiproliferative, apoptotic and antioxidant activities of punicalagin, ellagic acid and a total pomegranate tannin extract are enhanced in combination with other polyphenols as found in pomegranate juice

Seeram

Adams

Henning

et al. 2005

The Journal of Nutritional Biochemistry

954

664

View full text Add to dashboard Cite

“…In the present study, total phenolic content was higher than in similar Egyptian study which the extract had 6.2 mg GAE/g dry solids 44 , while in another studies in India the total phenolic content was 124.3 mg GAE/g dry solids 45,46 . Also, Yamani cultivar was found to have high total phenolics (91.2 mg GAE/g dry solids) 47 .This variation of total phenolics could associated with the difference in cultivars, methods of extraction and environmental conditions such as relative humidity and temperature of extracts.…”

Section: Discussioncontrasting

confidence: 76%

A Study on Biofilms Inhibition and Preservative Activity of Pomegranate Peel Methanol Extract (PPME)

Assar

Shahate

2017

Journal of Advanced Pharmacy Research

View full text Add to dashboard Cite

Objectives: Microorganisms with biofilms are associated with chronic human infections (highly resistant to antimicrobial agents). Pomegranate is used in treatment of several diseases, as its peels contain phenolic compounds and hydrolysable tannins. We aimed to study the effect of PPME on inhibition of biofilms and as a natural preservative in food industrial application. Methods: Pomegranate peel was extracted with methanol. E. coli clinical isolates were identified by standard microbiological procedures. Antibiotic susceptibility testing was performed using disc diffusion method. Antibacterial activity of PPME was tested using agar well diffusion assay. Minimum Inhibitory Concentration (MIC) was evaluated according to The European Committee on Antimicrobial Susceptibility Testing (EUCAST) agar dilution method. Biofilms detection was done by Tube method. Total phenolic content (TPC) in PPME was determined using spectrophotometric method. PPME ability to scavenge (1, 1diphenyl-2-picryl hydrazyl) DPPH free radicals was assessed by the standard method. Total antioxidant capacity of PPME was evaluated by phosphormolybdenum method. Total Phenolic Content (TPC) for cooked patties was freshly analyzed at different concentrations (1-0.25mg/ml). Results: Tested isolates were multidrug resistant. High antibacterial activity of PPME was found. MIC of PPME was found to be 10mg/ml. Tested isolates were biofilms producers, PPME largely affected on biofilm forming ability. TPC for treated group was (2785.19-349.22 µg/kg): for untreated group was 1251-226 µg/kg). DPPH radical scavenging activity was 38.12-5.45%. Thiobarbituric Acid Reactive Substances (TBARS) values were measured for samples from (0 -9 days) of storage period which was 0.522-1.3,0.859-1.816mg malonladehyde/kg meat for treated, untreated group respectively. AOA% was 31.49-66.92 from (0 -9) days of storage. Conclusions: PPME was able to inhibit biofilm formation in tested isolates and can be promising in inhibiting contamination caused by biofilm forming bacteria in food industry.

show abstract

“…The antioxidant activities of P. granatum are associated with different bioactive components, mainly polyphenols, ellagitannins, condensed tannins, and anthocyanins (Kulkarni et al, 2004;Li et al, 2006;Negi et al, 2003;Noda et al, 2002). In this regard, West et al (2007) showed that polyphenols present in this plant protect neonatal mouse brain against hypoxic-ischemic injury.…”

Section: Discussionmentioning

confidence: 99%

Assessment of mutagenic and antimutagenic effects of Punica granatum in mice

Valadares

Pereira

Benfica

et al. 2010

Braz. J. Pharm. Sci.

View full text Add to dashboard Cite

In the present study, the ability of Punica granatum ethanolic leaf extract (PGL) and Punica granatum ethanolic fruit extract (PGF) to induce mutagenicity or to modulate the genotoxic effects induced by the alkylating agent cyclophosphamide (CP) was evaluated. Swiss male mice were treated by gavage for 10 days with PGL or PGF (12.5, 25, 50, and 75 mg/kg/day) prior to exposure to CP (i.p. 200 mg/kg), 24 h after the end of the treatment. Initial observations revealed that normal mice treated with both extracts (12.5, 25, 50, and 75 mg/kg/day) showed a similar micronucleated polychromatic erythrocyte (MNPCE) frequency to that of the control group. Investigation of the protective effect of PGL and PGF based on data analysis revealed that, irrespective of dose or extract, oral administration of PGL or PGF for 10 days prior to exposure had reduced, in a dose-dependent manner, the frequency of MNPCE induced by CP in all groups studied. Higher reductions were observed at PGF doses of 50 and 75 mg/kg. Taken together, these results demonstrate that mice treated with P. granatum showed an absence of mutagenic effects and dose-dependent protective effects against CP-induced oxidative DNA damage. Uniterms:Punica granatum/mutagenic effects. Punica granatum/antimutagenic effects. Micronucleic. Genotoxicity. Cyclophosphamide.No presente estudo investigamos o potencial do extrato etanólico das folhas da Punica granatum (PGFO) e do extrato etanólico dos frutos da Punica granatum (PGFR) de induzir mutagenicidade ou de proteger contra efeitos genotóxicos induzidos pela ciclofosfamida (CF). Camundongos machos Swiss foram tratados por 10 dias, via oral, com PGFO ou PGFR (12,5, 25, 50 e 75 mg/kg/dia), previamente a exposição à CF (i.p. 200 mg/kg) 24 horas após término do tratamento. Observamos que os animais tratados por 10 dias com ambos os extratos (12,5, 25, 50 e 75 mg/kg/dia) demonstraram a frequência de micronúcleo policromático eritrocitário (MNPCE) similar ao grupo controle. Quando aos efeitos protetores dos extratos foram investigados, a análise dos dados revelou que, independentemente da dose ou do extrato usado, a administração oral por 10 dias, previamente à exposição, reduziu, de forma dose-dependente, a frequência de MNPCE induzidos pela CF, em todos os grupos estudados. As maiores reduções foram observadas com PGFR nas doses de 50 e 75 mg/kg. Em conjunto, sob as condições testadas, camundongos tratados com P. granatum demonstraram ausência de efeitos mutagênicos e, de forma dose-dependente, efeitos protetores contra os danos oxidativos do DNA induzidos pela CF. Unitermos:Punica granatum/efeitos mutagênicos. Punica granatum/efeitos antimutagênicos. Micronúcleo. Genotoxicidade. Ciclofosfamida. INTRODUCTIONPunica granatum Linn., a Punicaceae, commonly known as pomegranate, has a vast ethnomedical history and represents a phytochemical reservoir of heuristic medicinal value (Lansky and Newman, 2007). The plant as a whole and especially its flowers, have been extensively used in the ancient Ayurvedic health care system (Siv...

show abstract

Isolation and identification of a radical scavenging antioxidant – punicalagin from pith and carpellary membrane of pomegranate fruit

Cited by 180 publications

References 14 publications

In vitro antiproliferative, apoptotic and antioxidant activities of punicalagin, ellagic acid and a total pomegranate tannin extract are enhanced in combination with other polyphenols as found in pomegranate juice

In vitro antiproliferative, apoptotic and antioxidant activities of punicalagin, ellagic acid and a total pomegranate tannin extract are enhanced in combination with other polyphenols as found in pomegranate juice

A Study on Biofilms Inhibition and Preservative Activity of Pomegranate Peel Methanol Extract (PPME)

Assessment of mutagenic and antimutagenic effects of Punica granatum in mice

Contact Info

Product

Resources

About