Isolation and Hypoglycemic Activity of Aconitans A, B, C and D, Glycans ofAconitum carmichaeliRoots¹

Abstract

tracted with MeOH-water (1: 1, 501 x 4) for 4 days (each extraction) at room temperature. The aqueous MeOH solution was concentrated under reduced pressure to give the extract (2.7 kg) which was chromatographed over cellulose (5.0 kg). The active water eluate (410 g) was dialyzed with cellulose tubings (Visking, 36/32) against water for 10 days to afford the non-dialyzable portion (69.6 g). Successive chromatography of the nondialyzable portion over DEAE-cellulose (4.0 ID x 45 cm) with water, over Sepharose 6B (4.0 ID x 95 cm) with 0.1 N NaCI, over DEAE-Toyopearl 650M (4.0 ID x 45 cm) with water containing NaCl (0-* 1 M120 h) and over Sephacryl S-200 (4.0 ID x 95 cm) with 0.1 M Tris-HC1 buffer (pH 8.0) containing 0.5 M NaCI yielded moran A.

Phytochemicals for Diabetes Management

“…Many polysaccharides have been proven beneficial for the treatment of hypoglycaemia. Few polysaccharides and their mechanisms for antidiabetic action are listed below, Activity-guided fractionation of an aqueous methanol and aqueous extract of Aconitum carmichaeli Debeaux (Ranunculaceae) roots led to isolate four glycans, aconitans A, B, C and D. These glycans exhibited prominent hypoglycemic effects in normal and alloxan-produced hyperglycemic mice and markedly reduced the blood sugar level[134].…”

Foodinformatics

“…The paper by Tanaka et al [168] investigates small world phenomena in several libraries obtained directly from the ZINC DB [184] and from virtual libraries constructed from structurally diverse fragments. By contrast, the paper of Krein and Sukumar [169] undertakes a much more comprehensive analysis based on a number of different sets of CS descriptors applied not only to CSs but also to their subspaces associated with activity cliffs.…”

Hypoglycemic activity of polysaccharide fractions containing ß-glucans from extracts of Rhynchelytrum repens (Willd.) C.E. Hubb., Poaceae

“…Plasma glucose was determined by an enzymatic method (glucose-oxidase) before and 2, 4, 6, 8, and 24 h after administration of extracts. In both treatments, 4 M KOH and DGLUC, the animals received injections of 100 mg extract per kg body weight (24,25) and non-diabetic control rats and streptozotocin-induced diabetic control rats received saline solution. All extracts were administered intraperitoneally.…”