Isolation and growth of a bacterium able to degrade nitrilotriacetic acid under denitrifying conditions

Wanner, Ursula; Kemmler, Judith; Weilenmann, Hans Ulrich; Egli, Thomas; El-Banna, Tarek; Auling, Georg

doi:10.1007/bf00117049

Cited by 25 publications

(25 citation statements)

References 23 publications

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“…This study has shown for the first time that diethanolamine can be degraded without the involvement of molecular oxygen and confirms the finding of Wanner et al (1990) and others that amines can be degraded anaerobically under nitrate reducing conditions. The observation that growth of DEA4 is more rapid under anaerobic conditions than aerobic is unexpected, but might be due to inhibition by oxygen or oxygen metabolites in the aerobic cultures.…”

Section: Discussionsupporting

confidence: 72%

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The anaerobic biodegradation of diethanolamine by a nitrate reducing bacterium

1996

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The ability of bacterial cultures to degrade diethanolamine under anoxic conditions with nitrate as an electron acceptor was investigated. A mixed culture capable of anaerobic degradation of diethanolamine was obtained from river sediments by enrichment culture. From this a single bacterial strain was isolated which could use diethanolamine, monoethanolamine, triethanolamine and N-methyl diethanolamine as its sole carbon and energy sources either aerobically or anaerobically. Growth on diethanolamine was faster in the absence of oxygen. The accumulation of possible metabolites in the culture medium was determined as was the ability to grow on certain putative intermediates in the degradation of diethanolamine. A possible pathway for the degradation of ethanolamines by this organism is suggested.

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Section: Discussionsupporting

confidence: 72%

“…Enfors & Molin 1973 a,b;Egli & Weilenmann 1986). An organism has now been isolated in pure culture which can degrade NTA under denitrifying conditions (Wanner et al 1990).…”

Section: Introductionmentioning

confidence: 99%

The anaerobic biodegradation of diethanolamine by a nitrate reducing bacterium

1996

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“…We therefore used titanium(II1) as reductant (Zehnder & Wuhrniann, 1976), and we used a modification of the original procedure, with citrate as the anion, involving the less widely utilized nitrilotriacetate (NTA). We have unintentionally confirmed the ease with which anoxic degraders of NTA can be isolated (Wanner et al, 1990), and we foresee a further alteration of the medium to using EDTA, in place of NTA, to try to obtain isolates that are able to utilize the naturally occurring methanesulfonate (Baker et al, 1991), whose degradation in the anaerobic digestor is obviously less common than that of taurine, if it occurs at all.…”

Section: Discussionmentioning

confidence: 96%

Anaerobic taurine oxidation: a novel reaction by a nitrate-reducing Alcaligenes sp.

1997

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Enrichment cultures were prepared under strictly anoxic conditions in medium representing fresh water and containing an organosulfonate as electron donor and carbon source, and nitrate as electron acceptor. The inoculum was from the anaerobic digestor of two communal sewage works. The natural organosulfonates 2-aminoethanesulf onate (taurine), ~~-2-amino-3-sulfopropionate (cysteate) and 2-hydroxyethanesulf onate (isethionate) all gave positive enrichments, whereas unsubstituted alkanesulfonates, such as methanesulfonate and arenesulfonates, gave no enrichment. Two representative enrichments were used to obtain pure cultures, and strains NKNTAU (utilizing taurine) and NKNIS (utilizing isethionate) were isolated. Strain NKNTAU was examined in detail. Out of 18 tested organosulfonates, it utilized only one, taurine, and was identified as a novel AIcaligenes sp., a facultatively anaerobic bacterium. Carbon from taurine was converted to cell material and carbon dioxide. The amino group was released as ammonium ion and the sulfonate moiety was recovered as sulfate. Nitrate was reduced to nitrogen gas.

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“…NTA degradation was shown to be a result of microbial processes rather than of photochemical degradation (1,3,6,17,29,33). Several microorganisms, either obligately aerobic or facultatively denitrifying, that can use NTA as a sole source of carbon, nitrogen, and energy have been isolated (3,10,19,23,30,46,53). The majority of the obligately aerobic isolates has been assigned to two new genera, Chelatobacter and Chelatococcus (2).…”

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confidence: 99%

Cloning and characterization of the genes encoding nitrilotriacetate monooxygenase of Chelatobacter heintzii ATCC 29600

1996

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A 6.2-kb DNA fragment containing the genes for the nitrilotriacetate (NTA) monooxygenase of Chelatobacter heintzii ATCC 29600 was cloned and characterized by DNA sequencing and expression studies. The nucleotide sequence contained three major open reading frames (ORFs). Two of the ORFs, which were oriented divergently with an intergenic region of 307 bp, could be assigned to the NTA monooxygenase components A and B. The predicted N-terminal amino acid sequences of these ORFs were identical with those determined for the purified components. We therefore named these genes ntaA (for component A of NTA monooxygenase) and ntaB (for component B). The ntaA and ntaB genes could be expressed in Escherichia coli DH5␣, and the gene products were visualized after Western blotting (immunoblotting) and incubation with polyclonal antibodies against component A or B. By mixing overproduced NtaB from E. coli and purified component A from C. heintzii ATCC 29600, reconstitution of a functional NTA monooxygenase complex was possible. The deduced gene product of ntaA showed only significant homology to SoxA (involved in dibenzothiophene degradation) and to SnaA (involved in pristamycin synthesis); that of ntaB shared weak homologies in one domain with other NADH:flavine mononucleotide oxidoreductases. These homologies provide no conclusive answer as to the possible evolutionary origin of the NTA monooxygenase. The deduced gene product of the third ORF (ORF1) had homology in the N-terminal region with the GntR class of bacterial regulator proteins and therefore may encode a regulator protein, possibly involved in regulation of ntaA and ntaB expression.

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Isolation and growth of a bacterium able to degrade nitrilotriacetic acid under denitrifying conditions

Cited by 25 publications

References 23 publications

The anaerobic biodegradation of diethanolamine by a nitrate reducing bacterium

The anaerobic biodegradation of diethanolamine by a nitrate reducing bacterium

Anaerobic taurine oxidation: a novel reaction by a nitrate-reducing Alcaligenes sp.

Cloning and characterization of the genes encoding nitrilotriacetate monooxygenase of Chelatobacter heintzii ATCC 29600

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