Isolation and Growth Characteristics of SARS-CoV-2 in Vero Cell

Yao, Pingping; Zhang, Yachun; Sun, Yisheng; Gu, Yulin; Fang, Xu; Su, Bo; Chen, Chen; Lu, Hang-Jing; Wang, Dehui; Yang, Zhangnv; Niu, Biao; Chen, Jiancai; Xie, Lin; Chen, Lei; Wang, Hui; Zhao, Yuying; Guo, Yue; Ruan, Juncheng; Zhang, Zhu; Fu, Zhenfang; Tian, Dayong; An, Q.; Jiang, Jianmin; Zhu, Han-Ping

doi:10.1007/s12250-020-00241-2

Cited by 14 publications

(13 citation statements)

References 8 publications

(9 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It is critical to study the SARS-CoV-2 variants as well as their infectivity, pathogenicity, and immunogenicity. We previously reported a SARS-CoV-2 variant with a 12-bp deletion in the E gene [9]. Interestingly, by using plaque purification, we isolated two SARS-CoV-2 strains from the same patient's specimen: the F8 strain containing the mutant E gene and the 8X strain containing the wild type E gene.…”

Section: Discussionmentioning

confidence: 99%

“…The plaque reduction neutralization test (PRNT) was performed using the 12# SARS-CoV-2 virus strain [9]. Serum samples from immunized mice were inactivated at 56°C for 0.5 h and diluted serially.…”

Section: Neutralization Assaymentioning

confidence: 99%

“…Both strains were sequenced and a 12-bp deletion in the E gene at the position of 26320-26331 was found in the F8 strain, while no deletion in the E gene was detected in the 8X strain. We performed a sequence alignment of these sequences with the E gene sequences from Wuhan-Hu-1, SARS-CoV Tor2, bat coronavirus RaTG13 strains, and a SARS-CoV-2 strain (8#) isolated from the same patient without plaque purification previously [9] (Figure 1(A)). Apart from the deletion section (26320-26331), other nucleotide sequences were the same among the four SARS-CoV-2 virus strains.…”

Section: A 12-bp Deletion Of the E Gene Found In The Sars-cov-2 Virusmentioning

confidence: 99%

“…A SARS virus lacking the E gene would have a lower viral titer, and this variant strain might be used for the development of attenuated SARS vaccines [8]. We previously reported a SARS-CoV-2 virus strain with a 12-bp deletion in the E gene [9]. To date, no other studies about E gene deletion or mutations of SARS-CoV-2 have been reported.…”

mentioning

confidence: 99%

See 3 more Smart Citations

A SARS-CoV-2 variant with the 12-bp deletion at E gene

Sun

Fang

An³

et al. 2020

Emerging Microbes & Infections

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Neutralization Assaymentioning

confidence: 99%

Section: A 12-bp Deletion Of the E Gene Found In The Sars-cov-2 Virusmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

A SARS-CoV-2 variant with the 12-bp deletion at E gene

Sun

Fang

An³

et al. 2020

Emerging Microbes & Infections

Self Cite

View full text Add to dashboard Cite

show abstract

“…Several cell lines can support SARS-CoV-2 replication. [36][37][38][39] In fact, any cell line that expresses the cognate angiotensin-converting enzyme 2 and capable of sustaining replication of the virus can be used to assess infectivity. 40 Among the most commonly used cell lines are Vero and its derivatives.…”

Section: Infectivity Of Blood Components and Other Nonrespiratory Smentioning

confidence: 99%

Risk of transmission of severe acute respiratory syndrome coronavirus 2 by transfusion: A literature review

et al. 2020

View full text Add to dashboard Cite

Severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) is a novel human coronavirus responsible for coronavirus disease 2019 (COVID‐19). The emergence of this virus in Wuhan (China) at the end of 2019, and its worldwide spread to reach the pandemic stage, has raised concerns about the possible risk that it might be transmissible by transfusion. This theoretical risk is further supported by reports of the detection of viral RNA in the blood of some infected individuals. To further address this risk, a thorough PubMed literature search was performed to systematically identify studies reporting data on the detection of SARS‐CoV‐2 RNA in blood or its components. Complementary searches were done to identify articles reporting data on the in vitro infectivity of blood components. At least 23 articles presenting data on the detection of SARS‐CoV‐2 RNA in blood, plasma, or serum were identified. Of these, three studies reported on blood donors with COVID‐19 infection identified post‐donation, and no cases of transfusion transmission were identified. A few studies mentioned results of in vitro infectivity assays of blood components in permissive cell lines, none of which were able to detect infectious virus in blood or its components. Complementary searches have identified reports demonstrating that the correlation between the presence of viral RNA in a biological sample and infectivity requires a minimal RNA load, which is rarely, if at all observed, in blood components. Overall, the available evidence suggests that the risk of transmission of SARS‐CoV‐2 by transfusion remains theoretical.

show abstract