Isolation and Genotyping of
            <i>Helicobacter pylori</i>
            from Untreated Municipal Wastewater

Lu, Yingzhi; Redlinger, Thomas; Avitia, Raquel; Galindo, Adriana; Goodman, Karen J.

doi:10.1128/aem.68.3.1436-1439.2002

Cited by 166 publications

(138 citation statements)

References 23 publications

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“…O H. pylori pode sobreviver por mais de 7 dias na água 24 , segundo um estudo que relatou o cultivo bem sucedido da bactéria em água potável 25 . Assim, o consumo de água contaminada deve ser considerado uma fonte potencial de transmissão do H. pylori 26 .…”

Section: Discussionunclassified

Prevalence of Helicobacter pylori infection and intestinal parasitosis in children of the Xingu Indian Reservation

Escobar-Pardo¹,

Godoy²,

Machado³

et al. 2011

J Pediatr (Rio J)

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Objective: To evaluate the prevalence of Helicobacter pylori infection and its association with intestinal parasitoses in children from indigenous communities of the Xingu Indian Reservation, in Brazil.Methods: A total of 245 Native Brazilian children between 2 and 9 years of age, from six villages of the Xingu River region, a tributary of the Amazon River, were assessed. H. pylori was detected using the 13 C-urea breath test. Breath samples were collected at baseline and 30 minutes after ingestion of 50 mg of 13 C-urea diluted with 100 mL of water flavored with passion fruit juice and sweetener. Stool samples were collected for the stool ova and parasites exam for 202/245 (82.4%) children. Results:The overall prevalence of H. pylori was 73.5%. A significant association of H. pylori with increased age was observed among the different villages and ethnic groups. Positive results for the presence of parasites -97.5% (198/202) -from the stool samples collected showed no association with H. pylori. Giardia showed an association with H. pylori in the multivariate analysis. Risk factors for H. pylori infection were observed in Kisêjê and Kaibi ethnic groups (OR [odds ratio] = 3.36 and 4.00, respectively), as well as in Tuiararé, Ngojwere, Capivara, Diauarum, and Pavuru villages (OR = 8.10, 4.10, 4.88, 1.85, and 1.40, respectively). Conclusions:H. pylori infection is highly prevalent in these communities, as well as intestinal parasitoses. However, there were significant differences in the prevalence of H. pylori among the diverse villages studied. Giardia was closely associated with H. pylori infection. J Pediatr (Rio J). 2011;87(5):393-8: Epidemiology, prevalence,Helicobacter pylori, giardia. ResumoObjetivo: Avaliar a prevalência da infecção por Helicobacter pylori e sua associação com parasitoses intestinais em crianças da comunidade indígena do Parque Indígena do Xingu. Métodos:Foram incluídas 245 crianças indígenas entre 2 e 9 anos, de seis aldeias da região do rio Xingu, afluente do Amazonas. H. pylori foi detectado pelo teste respiratório com ureia-13 C. Foram coletadas amostras de ar expirado, em jejum e 30 minutos após a ingestão de 50 mg de ureia-13 C diluída em 100 mL de água aromatizada com suco de maracujá. Foram coletadas amostras de fezes de 202/245 (82,4%) crianças para exame protoparasitológico. Conclusões: Foi encontrada alta prevalência de H. pylori e de parasitose intestinal em crianças nas comunidades presentemente investigadas. No entanto, houve diferença significativa na prevalência do H. pylori entre as diversas aldeias estudadas. Verificou-se associação entre a presença de giárdia e a infecção por H. pylori.

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Section: Discussionunclassified

Prevalence of Helicobacter pylori infection and intestinal parasitosis in children of the Xingu Indian Reservation

Escobar-Pardo¹,

Godoy²,

Machado³

et al. 2011

J Pediatr (Rio J)

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“…The final identification of H. pylori was confirmed by using 16S rDNA gene PCR by species-specific primers as shown in Table 2 (Lu et al, 2002). The second PCR was done in order to detect the target genes which were cagH, cagL, cagG, and orf17 ( Table 2).…”

Section: Pcr Amplificationmentioning

confidence: 99%

Helicobacter pylori cag Pathogenicity Island cagL and orf17 Genotypes Predict Risk of Peptic Ulcerations but not Gastric Cancer in Iran

Raei¹,

Latifi‐Navid²,

Zahri³

2015

Asian Pacific Journal of Cancer Prevention

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Background: Gastric cancer (GC) is the third most common cancer regarding mortality in the world. The cag pathogenicity island (PAI) of Helicobacter pylori which contains genes associated with a more aggressive phenotype may involve in the pathogenesis of gastrointestinal disease. We here aimed to examine the associations of cagH, cagL, orf17, and cagG genotypes of H. pylori cag PAI with severe gastrointestinal disease. Materials and Methods: A total of 242 H. pylori strains were genotyped. Histopathological examination and classification of subjects were performed. Results: The frequencies of the cagH, cagL, cagG, and orf17 genotypes were 40/54 (74.1%), 53/54 (98.1%), 38/54 (70.4%), and 43/54 (79.6%), respectively, in patients with peptidic ulceration (PU),while in the control group, the frequencies were 87/147 (59.6%) for cagH, 121/146 (82.9%) for cagL, 109/146 (74.7%) for cagG, and 89/146 (61.0%) for orf17. The results of simple logistic regression analysis showed that the cagL and orf17 genotypes were significantly associated with an increased risk of PU not GC; the ORs (95% CI) were 10.950 (1.446-82.935), and 2.504 (1.193-5.253), respectively. No significant association was found between the cagH and cagG genotypes and the risk of both the PU and the GC in Iran (P>0.05). Finally, multiple logistic regression analysis showed that the cagL genotype was independently and significantly associated with the ageand sex-adjusted risk for PU; the OR (95% CI) was 9.557 (1.219-17.185). Conclusions: We conclude that the orf17 and especially cagL genotypes of H. pylori cag PAI could be factors for risk prediction of PU, but not GC in Iran.

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“…All primer sets were selected from published literature (Lu et al, 2002;Ogiwara et al, 2009). The PCR assay was performed in a reaction volume of 25μL using a commercially available kit (CinnaGen, Iran).…”

Section: Primers and Pcr Conditionsmentioning

confidence: 99%

Helicobacter pylori vacA d1 Genotype Predicts Risk of Gastric Adenocarcinoma and Peptic Ulcers in Northwestern Iran

Basiri¹,

Safaralizadeh²,

Bonyadi³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Background: There is a close relationship between Helicobacter pylori (H pylori)-specific factors and different gastroduodenal diseases. The present study aimed to investigate the prevalence of vacA d1, d2 genotypes in the H pylori isolates from patients with gastric adenocarcinoma, peptic ulcer disease (PUD) and gastritis in East Azerbaijan region, where the incidence of gastric cancer (GC) is high. Strains isolated from this area are likely to be of European ancestry. Materials and Methods: In this study, genotyping of the vacA d region of 115 isolates obtained from patients with different gastrodoudenal diseases was accomplished by PCR methods. In addition to PCR amplification of H pylori 16S rDNA, rapid urease tests or histological examination were used to confirm the presence of H pylori in biopsy specimens. Data were collected and analyzed using SPSS version 19. Results: Of the total of 83 H pylori isolates, 36 (43.4%) contained the d1 allele and 47 (56.6%) were subtype d2. The results of the multiple linear/logistic regression analysis showed high correlation between allele d1 and gastric adenocarcinoma or PUD. Conclusions: This study suggests that the H pylori vacA d1 genotype helps predict risk for gastric adenocarcinoma and PUD in East Azerbaijan, Iran.

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Isolation and Genotyping of Helicobacter pylori from Untreated Municipal Wastewater

Cited by 166 publications

References 23 publications

Prevalence of Helicobacter pylori infection and intestinal parasitosis in children of the Xingu Indian Reservation

Prevalence of Helicobacter pylori infection and intestinal parasitosis in children of the Xingu Indian Reservation

Helicobacter pylori cag Pathogenicity Island cagL and orf17 Genotypes Predict Risk of Peptic Ulcerations but not Gastric Cancer in Iran

Helicobacter pylori vacA d1 Genotype Predicts Risk of Gastric Adenocarcinoma and Peptic Ulcers in Northwestern Iran

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