Isolation and genotyping of Candida albicans involved in vaginal candidiasis among pregnant women in Sulaymaniyah and Erbil cities

Shekhany, Khattab Ahmed Mustafa

doi:10.15218/zjms.2021.012

Cited by 6 publications

(5 citation statements)

References 22 publications

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“…Studies have shown that C. albicans is responsible for 80-92% of yeast infections; however, our findings are in contrast to the known prevalence of Candida sp. infection, accounting for around 67.7% and 56.14% prevalence, respectively (Ghajari et al 2018;Shekhany 2021). It was previously believed that Candida only passively aids in developing an opportunistic fungal infection in the presence of an organic weakness or an immunocompromised host.…”

Section: Identification Of Candida Spp Isolatesmentioning

confidence: 99%

Molecular determination of the microbial diversity associated with vaginitis and testing their sensitivity to selected antimicrobials

AL-ZAIDI,

AL-TAMIMI,

SALEH

2023

Biodiversitas

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Abstract. Al-Zaidi MHH, Al-Tamimi WH, Saleh AAA. 2023. Molecular determination of the microbial diversity associated with vaginitis and testing their sensitivity to selected antimicrobials. Biodiversitas 24: 4253-4261. Millions of women suffer from bacterial vaginitis is associated with several serious health problems. The present study aimed to investigate the microbial diversity and their sensitivity to different antimicrobial agents. Bacterial isolates were identified by genotyping using the 16S rRNA gene sequencing and BLASTn analysis, while conventional mycological methods identified Candida spp. The disc diffusion method was used to study the antimicrobial susceptibility patterns. The molecular identification showed that Escherichia coli (20.8%) and Staphylococcus haemolyticus (20.8%) were the most frequent species, followed by Klebsiella pneumoniae (16.7%). At the same time, Macrococcus caseolyticus, Streptococcus agalactiae, Staphylococcus aureus, and Bacillus cereus were the least prevalent bacteria (4.16%)—all Candida sp. against the selected antimicrobial agents. Thus, molecular assays are important in monitoring microorganisms associated with vaginitis. Prospective genotyping studies are needed to determine these microbes' resistant genes and understand their mode of action and response to drug therapy.

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Section: Identification Of Candida Spp Isolatesmentioning

confidence: 99%

Molecular determination of the microbial diversity associated with vaginitis and testing their sensitivity to selected antimicrobials

AL-ZAIDI,

AL-TAMIMI,

SALEH

2023

Biodiversitas

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“…Then place in the thermocycler for 20minute at 95⁰ C, followed by centrifugation for 2 min at 12,000 rpm. The DNAcontaining supernatant was collected and stored at -20 °C for subsequent steps of PCR [21]. The universal primers, forward ITS1-F (5′-TCCGTAGGTGAACCTGCG-3′) and reverse ITS4-R (5′ TCCTCCGCTTATTGATATGC-3′), were utilized to amplify the intergenic spacer regions (ITS) of 5.8 S ribosomal DNA (rDNA) [22].…”

Section: Molecular Identification Of Candida Isolatesmentioning

confidence: 99%

“…For further validation, all the amplicons previously amplified using ITS primers and were 532 bp in size were re-analyzed using a different set of primers CHS1. The chitin synthase gene (CHS1) from C. albicans, 122bp in size, can be amplified by conventional PCR [21]. The colony PCR was carried out with the same procedure previously mentioned using forward CHS1-F (5'-CGCCTCTGATGGTGATGAT-3') and reverse CHS1-R (5'-TCCGGTATCACCTGGCTC-3') primers [23].…”

Section: Confirmation Of the Candida Albicans By Detection Of Chs1 Genementioning

confidence: 99%

Molecular identification of Candida spp. isolated from women withvulvovaginal candidiasis in Koya District, Iraq

Mohammed,

Zrary

2023

JZS

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Vulvovaginal candidiasis (VVC) is the second most common vaginal infection among women of reproductive age. It is an opportunistic mucosal infection. Candida species causes VVC, which affects millions of women annually. The objective of this study was to isolate and identify the species of Candida from married women who had VVC attending Shahid Doctor Khalid Hospital & Haji Qader Health Centre in Koya, Iraq using traditional and molecular methods. This research was carried out, from December 2021 to May 2022; 150 vaginal swabs from married patients with symptoms of VVC were collected. Swabs were microscopically examined, grown on Sabouraud Dextrose Agar (SDA), and HiCrome™ Candida Differential agar and Germ Tube Test were used to identify Candida isolates. Next, the results were validated using the molecular method called polymerase chain reaction (PCR) to amplify and detect the ITS region in this study. The chitin synthase gene (CHS1) confirmed all C. albicans isolates. Out of the150 vaginal swabs gathered, 84 (56%) cases were positive for vaginal candidiasis. Ages26-30 were found to have the most significant proportion of positive patients, and Women who were pregnant had a more positive culture than women who were not. Identifying six different species of Candida was accomplished through the application of both traditional and molecular techniques in the study; they are C. albicans (88.1%), C. glabrata (4.8%), C. kefyr (2.4%), C. krusei (2.4%), C. parapsilosis (1.2%), and C. tropicalis (1.2%). Finally, we concluded that VVC was shown to be prevalent in Koya city, with the predominant species as C. albicans among all other species.

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“…Another recent study conducted at Maternity Hospital in Erbil City; Iraq found that the prevalence of vulvovaginal candidiasis using culture was 54.66%. The highest number of positive cases 71 (63.4%) was found among the age group of 30 -34 years (Shekhany, 2021) The absolute diagnosis of C. albicans depends on the clinical manifestations is difficult due to mixed with other infections affecting vaginal, including Bacterial vaginosis, Neisseria gonorrhoea and Trichomonas vaginalis. Thus, mycological examinations of vaginal discharges are critical for the initial diagnosis of infection and detection of definitive causative agents (Anderson et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Candida Albicans and Its Associated Risk Factors Among Married Women of Reproductive Age in Zakho City, Kurdi-Stan Region, Iraq

Haydar

Naqid

2023

SJUOZ

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Background: Candida vaginitis is a major fungal infection of the female genital tract ranging from mucosal to systemic infection. Candida species has remained a major public health issue affecting women globally. Objectives: The aim of this study was to evaluate the prevalence of Candida albicans and its associated risk factors among married women with signs and symptoms of vaginitis referring to Obstetrics and Gynecological Hospital at Zakho City, Iraq. Methods: Vaginal samples were collected from 150 symptomatic women aged 18-48 years from October 2021-to April 2022 using two sterile cotton swabs. Wet mount was initially performed from first swab for the presence of budding yeast cells, and pseudo-hyphae of Candida infection. The second collected swabs were cultured on Sabouraud dextrose agar and incubated at 35°C for 24-48 hrs. C. albicans was then detected using morphologic characteristics of the colony. Candida colonies were finally confirmed by germ tube test. Results: Overall, 58 (38.67%) C. albicans were diagnosed. The highest rate of infection was reported in the age group of 31-40 years (45%) and non-educated people (37%), but statistically not significant (p>0.05). C. albicans was also higher in women who used pills as a contraceptive (45.45%) than other types of contraceptives. Women who had low number of births were significantly associated with C. albicans (OR; 1.01, 95% CI; 0.94-1.26, p=028). C. albicans was also significantly associated among symptomatic patients with vaginal itching (67.19%) (OR; 9.69, 95% CI; 4.52-20.79, p=0.001) and vaginal pH level > 4.5 (OR; 0.39, 95% CI; 0.19-0.82, p=0.001). C. albicans was also significantly higher among participants who had whitish vaginal discharges (96.67%) (OR; 8.0, 95%CI, 3.8-16.85, p=0.001), and yellow-green vaginal discharges (58.62%) (OR; 0.12, 95% CI; 0.01-0.93, p=0.007). Conclusions: C. albicans is a relatively common gynaecological problem among married women in our region. C. albicans is significantly higher among child bearing age women who had vaginal discharges and itching in our study. These results call for preventive measures to protect women against Candidiasis. Therefore, public health education and campaign should be implemented.

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Isolation and genotyping of Candida albicans involved in vaginal candidiasis among pregnant women in Sulaymaniyah and Erbil cities

Cited by 6 publications

References 22 publications

Molecular determination of the microbial diversity associated with vaginitis and testing their sensitivity to selected antimicrobials

Molecular determination of the microbial diversity associated with vaginitis and testing their sensitivity to selected antimicrobials

Molecular identification of Candida spp. isolated from women withvulvovaginal candidiasis in Koya District, Iraq

Evaluation of Candida Albicans and Its Associated Risk Factors Among Married Women of Reproductive Age in Zakho City, Kurdi-Stan Region, Iraq

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