Isolation and genomic characterization of five novel strains of Erysipelotrichaceae from commercial pigs

Wu, Jinyuan; Liu, Min; Zhou, Meng; Wu, Lin; Yang, Hui; Huang, Lusheng; Chen, Congying

doi:10.1186/s12866-021-02193-3

Cited by 36 publications

(21 citation statements)

References 73 publications

(37 reference statements)

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“…N -Lauroylglycine and 2-aminooctanoic acid, two fatty acid metabolites, had negative and positive correlations with ADG, respectively. As a lipoamino acid in the cell membrane of various bacteria, N -lauroylglycine in mouse feces has been positively correlated with Erysipelotrichaceae [ 35 ], which is a family of Gram-positive bacteria with the genomic characteristic of a polysaccharide utilization function [ 36 ]. As for 2-aminooctanoic acid, it has been detected in the serum of pigs as a marker of ractopamine—a growth promoter [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

Identification of Independent and Shared Metabolic Responses to High-Fiber and Antibiotic Treatments in Fecal Metabolome of Grow–Finish Pigs

Hung

Song

et al. 2022

Metabolites

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Feeding high-fiber (HF) coproducts to grow–finish pigs as a cost-saving practice could compromise growth performance, while the inclusion of antibiotic growth promoters (AGPs) may improve it. The hindgut is a shared site of actions between fiber and AGPs. However, whether the metabolic interactions between them could occur in the digestive tract of pigs and then become detectable in feces have not been well-examined. In this study, wheat middling (WM), a HF coproduct, and bacitracin, a peptide antibiotic (AB), were fed to 128 grow–finish pigs for 98 days following a 2 × 2 factorial design, including antibiotic-free (AF) + low fiber (LF); AF + HF; AB + LF, and AB + HF, for growth and metabolic responses. The growth performance of the pigs was compromised by HF feedings but not by AB. A metabolomic analysis of fecal samples collected on day 28 of feeding showed that WM elicited comprehensive metabolic changes, especially in amino acids, fatty acids, and their microbial metabolites, while bacitracin caused selective metabolic changes, including in secondary bile acids. Limited metabolic interactions occurred between fiber and AB treatments. Moreover, the correlations between individual fecal metabolites and growth support the usage of fecal metabolome as a source of biomarkers for monitoring and predicting the metabolic performance of grow–finish pigs.

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Section: Discussionmentioning

confidence: 99%

Identification of Independent and Shared Metabolic Responses to High-Fiber and Antibiotic Treatments in Fecal Metabolome of Grow–Finish Pigs

Hung

Song

et al. 2022

Metabolites

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“…In the current study, dietary supplements with B. animalis promoted Erysipelotrichaceae_UCG-003 populations in both lumens of the ileum and cecum, which may positively affect the growth performance of pigs. Polysaccharide utilization loci analysis indicated that Erysipelotrichaceae strains may have the ability to utilize plant polysaccharides ( 43 ). Concordantly, pigs fed a diet with high corn-based arabinoxylans increased Erysipelotrichaceae_UCG-002 abundance.…”

Section: Discussionmentioning

confidence: 99%

Bifidobacterium animalis Promotes the Growth of Weaning Piglets by Improving Intestinal Development, Enhancing Antioxidant Capacity, and Modulating Gut Microbiota

Pang

Liu

Kang

et al. 2022

Appl Environ Microbiol

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“…Furthermore, other members of the Erysipelotrichaceae family, to which Ileibacterium belongs, have been reported as being intrinsically resistant to vancomycin. [45][46][47] Considering that mechanisms for resistance against glycopeptide antibiotics like vancomycin can differ greatly between taxa and remain to be fully characterized in all bacteria, 48 it is thus still possible that Ileibacterium is truly resistant to vancomycin.…”

Section: Discussionmentioning

confidence: 99%

Actively replicating gut bacteria identified by 5-ethynyl-2’-deoxyuridine (EdU) click chemistry and cell sorting

Beauchemin

Hunter

Maurice³

2022

Preprint

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The composition of the intestinal bacterial community is well described, but recent research suggests that the metabolism of these bacteria plays a larger role in health than which species are present. One fundamental aspect of gut bacterial metabolism that remains understudied is bacterial replication. Indeed, there exist few techniques which can identify actively replicating gut bacteria. In this study, we aimed to address this gap by adapting 5-ethynyl-2′-deoxyuridine (EdU) click chemistry (EdU-click), a metabolic labeling method, coupled with fluorescence-activated cell sorting and sequencing (FACS-Seq) to characterize replicating gut bacteria. We first used EdU-click with human gut bacterial isolates and show that many of them are amenable to this technique. We then optimized EdU-click and FACS-Seq for murine fecal bacteria and reveal that Prevotella UCG-001 and Ileibacterium are enriched in the replicating fraction. Finally, we labelled the actively replicating murine gut bacteria during exposure to cell wall-specific antibiotics in vitro. We show that regardless of the antibiotic used, the actively replicating bacteria largely consist of Ileibacterium, suggesting the resistance of this taxon to perturbations. Overall, we demonstrate how combining EdU-click and FACSeq can identify the actively replicating gut bacteria and their link with the composition of the whole community in both homeostatic and perturbed conditions. This technique will be instrumental in elucidating in situ bacterial replication dynamics in a variety of other ecological states, including colonization and species invasion, as well as for investigating the relationship between the replication and abundance of bacteria in complex communities.

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Isolation and genomic characterization of five novel strains of Erysipelotrichaceae from commercial pigs

Cited by 36 publications

References 73 publications

Identification of Independent and Shared Metabolic Responses to High-Fiber and Antibiotic Treatments in Fecal Metabolome of Grow–Finish Pigs

Identification of Independent and Shared Metabolic Responses to High-Fiber and Antibiotic Treatments in Fecal Metabolome of Grow–Finish Pigs

Bifidobacterium animalis Promotes the Growth of Weaning Piglets by Improving Intestinal Development, Enhancing Antioxidant Capacity, and Modulating Gut Microbiota

Actively replicating gut bacteria identified by 5-ethynyl-2’-deoxyuridine (EdU) click chemistry and cell sorting

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